Association of mitochondrial deoxyribonucleic acid mutation with polymorphism in CYP2E1 gene in oral carcinogenesis
ABSTRACT: Background: Oral carcinogenesis is a complex process affected by genetic as well as environmental factors. CYP2E1 gene is involved in metabolism of number of compounds and carcinogens. Its normal functioning is required for homeostasis of free radical. Mitochondrial deoxyribonucleic acid...
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| Format: | Article |
| Language: | English |
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Elsevier
2012-01-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426812600037 |
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| author | Rahul Pandey Divya Mehrotra Carlo Catapano Vimal Choubey Rajiv Sarin Abbas Ali Mahdi Stuti Singh |
| author_facet | Rahul Pandey Divya Mehrotra Carlo Catapano Vimal Choubey Rajiv Sarin Abbas Ali Mahdi Stuti Singh |
| author_sort | Rahul Pandey |
| collection | DOAJ |
| description | ABSTRACT: Background: Oral carcinogenesis is a complex process affected by genetic as well as environmental factors. CYP2E1 gene is involved in metabolism of number of compounds and carcinogens. Its normal functioning is required for homeostasis of free radical. Mitochondrial deoxyribonucleic acid (mtDNA) is 10–100 times more susceptible to damage than nuclear DNA. Mitochondrial DNA large scale deletions are well documented in oral cancer. However, the relationship between CYP2E1 gene polymorphisms and mtDNA damage is still not documented in literature. Materials and Methods: Case–control study involving 50 subjects was carried out. Deoxyribonucleic acid extraction was done from study subject tissue samples. Restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR) amplification was done to confirm CYP2E1 gene polymorphisms. The PCR amplification was done for mtDNA 4977 bp deletion. Statistical analysis was carried out using SPSS version 11.5 with χ2 tests. Results: c1c1 and DD polymorphisms are prevalent in North Indian population having oral cancer. These polymorphisms are significantly associated with mtDNA 4977 bp deletion. Conclusion: Mitochondrial DNA damage induced by wild CYP2E1 forms and imperfect DNA repair in mtDNA may act synergistically to greatly enhance oral cancer risk. |
| format | Article |
| id | doaj-art-d8b2426ab3854a65a85d5813d3d4db40 |
| institution | Kabale University |
| issn | 2212-4268 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-d8b2426ab3854a65a85d5813d3d4db402024-11-23T06:26:09ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682012-01-012149Association of mitochondrial deoxyribonucleic acid mutation with polymorphism in CYP2E1 gene in oral carcinogenesisRahul Pandey0Divya Mehrotra1Carlo Catapano2Vimal Choubey3Rajiv Sarin4Abbas Ali Mahdi5Stuti Singh6Senior Research Fellow, Department of Oral and Maxillofacial Surgery, CSM Medical University, Lucknow, IndiaProfessor, Department of Oral and Maxillofacial Surgery, CSM Medical University, Lucknow, India; Correspondence: Dr. Divya MehrotraProfessor and Director, Institute of Oncology Research, SwitzerlandResearch Scholar, Department of Urology, CSM Medical University, Lucknow, IndiaProfessor and Director, ACTREC, Mumbai, Maharashtra, IndiaProfessor and Head, Department of Biochemistry, CSM Medical University, Lucknow, IndiaResident, Department of Oral and Maxillofacial Surgery, CSM Medical University, Lucknow, IndiaABSTRACT: Background: Oral carcinogenesis is a complex process affected by genetic as well as environmental factors. CYP2E1 gene is involved in metabolism of number of compounds and carcinogens. Its normal functioning is required for homeostasis of free radical. Mitochondrial deoxyribonucleic acid (mtDNA) is 10–100 times more susceptible to damage than nuclear DNA. Mitochondrial DNA large scale deletions are well documented in oral cancer. However, the relationship between CYP2E1 gene polymorphisms and mtDNA damage is still not documented in literature. Materials and Methods: Case–control study involving 50 subjects was carried out. Deoxyribonucleic acid extraction was done from study subject tissue samples. Restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR) amplification was done to confirm CYP2E1 gene polymorphisms. The PCR amplification was done for mtDNA 4977 bp deletion. Statistical analysis was carried out using SPSS version 11.5 with χ2 tests. Results: c1c1 and DD polymorphisms are prevalent in North Indian population having oral cancer. These polymorphisms are significantly associated with mtDNA 4977 bp deletion. Conclusion: Mitochondrial DNA damage induced by wild CYP2E1 forms and imperfect DNA repair in mtDNA may act synergistically to greatly enhance oral cancer risk.http://www.sciencedirect.com/science/article/pii/S2212426812600037CYP2E1DNAmitochondriaoral cancer |
| spellingShingle | Rahul Pandey Divya Mehrotra Carlo Catapano Vimal Choubey Rajiv Sarin Abbas Ali Mahdi Stuti Singh Association of mitochondrial deoxyribonucleic acid mutation with polymorphism in CYP2E1 gene in oral carcinogenesis Journal of Oral Biology and Craniofacial Research CYP2E1 DNA mitochondria oral cancer |
| title | Association of mitochondrial deoxyribonucleic acid mutation with polymorphism in CYP2E1 gene in oral carcinogenesis |
| title_full | Association of mitochondrial deoxyribonucleic acid mutation with polymorphism in CYP2E1 gene in oral carcinogenesis |
| title_fullStr | Association of mitochondrial deoxyribonucleic acid mutation with polymorphism in CYP2E1 gene in oral carcinogenesis |
| title_full_unstemmed | Association of mitochondrial deoxyribonucleic acid mutation with polymorphism in CYP2E1 gene in oral carcinogenesis |
| title_short | Association of mitochondrial deoxyribonucleic acid mutation with polymorphism in CYP2E1 gene in oral carcinogenesis |
| title_sort | association of mitochondrial deoxyribonucleic acid mutation with polymorphism in cyp2e1 gene in oral carcinogenesis |
| topic | CYP2E1 DNA mitochondria oral cancer |
| url | http://www.sciencedirect.com/science/article/pii/S2212426812600037 |
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