Establishment of a visualized mouse orthotopic xenograft model of nasopharyngeal carcinoma
Mouse orthotopic xenograft tumor models are commonly employed in studies investigating the mechanisms underlying the development and progression of tumors and their preclinical treatment. However, the unavailability of mature and visualized orthotopic xenograft models of nasopharyngeal carcinoma lim...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
|
| Series: | Cancer Biology & Therapy |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/15384047.2024.2382531 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846126018475065344 |
|---|---|
| author | Wei Chen Wei-Min Chen Si-Xia Chen Li Jiang Ge-Ge Shu Yuan-Xiu Yin Zhi-Peng Quan Zi-Yan Zhou Ming-Jun Shen Ya-Ting Qin Chao-Lin Yang Xue-Jin Su Min Kang |
| author_facet | Wei Chen Wei-Min Chen Si-Xia Chen Li Jiang Ge-Ge Shu Yuan-Xiu Yin Zhi-Peng Quan Zi-Yan Zhou Ming-Jun Shen Ya-Ting Qin Chao-Lin Yang Xue-Jin Su Min Kang |
| author_sort | Wei Chen |
| collection | DOAJ |
| description | Mouse orthotopic xenograft tumor models are commonly employed in studies investigating the mechanisms underlying the development and progression of tumors and their preclinical treatment. However, the unavailability of mature and visualized orthotopic xenograft models of nasopharyngeal carcinoma limits the development of treatment strategies for this cancer. The aim of this study was to provide a simple and reliable method for building an orthotopic xenograft model of nasopharyngeal carcinoma. Human nasopharyngeal carcinoma (C666–1-luc) cells, stably expressing the firefly luciferase gene, were injected subcutaneously into the right axilla of BALB/C nude mice. Four weeks later, the resulting subcutaneous tumors were cut into small blocks and grafted into the nasopharynx of immunodeficient BALB/C nude mice to induce tumor formation. Tumor growth was monitored by bioluminescence imaging and small animal magnetic resonance imaging (MRI). The expression of histological and immunological antigens associated with orthotopic xenograft nasopharyngeal carcinoma was analyzed by tissue section analysis and immunohistochemistry (IHC). A visualized orthotopic xenograft nasopharyngeal carcinoma model was successfully developed in this study. Luminescence signal detection, micro-MRI, and hematoxylin and eosin staining revealed the successful growth of tumors in the nasopharynx of the nude mice. Moreover, IHC analysis detected cytokeratin (CK), CK5/6, P40, and P63 expression in the orthotopic tumors, consistent with the reported expression of these antigens in human nasopharyngeal tumors. This study established a reproducible, visual, and less lethal orthotopic xenograft model of nasopharyngeal carcinoma, providing a platform for preclinical research. |
| format | Article |
| id | doaj-art-d8ab25ddda6b4c52a2e97b0646860eff |
| institution | Kabale University |
| issn | 1538-4047 1555-8576 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cancer Biology & Therapy |
| spelling | doaj-art-d8ab25ddda6b4c52a2e97b0646860eff2024-12-13T06:53:24ZengTaylor & Francis GroupCancer Biology & Therapy1538-40471555-85762024-12-0125110.1080/15384047.2024.2382531Establishment of a visualized mouse orthotopic xenograft model of nasopharyngeal carcinomaWei Chen0Wei-Min Chen1Si-Xia Chen2Li Jiang3Ge-Ge Shu4Yuan-Xiu Yin5Zhi-Peng Quan6Zi-Yan Zhou7Ming-Jun Shen8Ya-Ting Qin9Chao-Lin Yang10Xue-Jin Su11Min Kang12Department of Radiation Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, ChinaDepartment of Radiation Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, ChinaDepartment of Radiation Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, ChinaDepartment of Radiation Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, ChinaMinistry of Education, Key Laboratory of Early Prevention and Treatment for Regional High Frequency Tumor (Guangxi Medical University), Nanning, Guangxi, ChinaDepartment of Radiation Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, ChinaMinistry of Education, Key Laboratory of Early Prevention and Treatment for Regional High Frequency Tumor (Guangxi Medical University), Nanning, Guangxi, ChinaDepartment of Radiation Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, ChinaDepartment of Radiation Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, ChinaDepartment of Radiation Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, ChinaDepartment of Radiation Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, ChinaDepartment of Radiation Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, ChinaDepartment of Radiation Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, ChinaMouse orthotopic xenograft tumor models are commonly employed in studies investigating the mechanisms underlying the development and progression of tumors and their preclinical treatment. However, the unavailability of mature and visualized orthotopic xenograft models of nasopharyngeal carcinoma limits the development of treatment strategies for this cancer. The aim of this study was to provide a simple and reliable method for building an orthotopic xenograft model of nasopharyngeal carcinoma. Human nasopharyngeal carcinoma (C666–1-luc) cells, stably expressing the firefly luciferase gene, were injected subcutaneously into the right axilla of BALB/C nude mice. Four weeks later, the resulting subcutaneous tumors were cut into small blocks and grafted into the nasopharynx of immunodeficient BALB/C nude mice to induce tumor formation. Tumor growth was monitored by bioluminescence imaging and small animal magnetic resonance imaging (MRI). The expression of histological and immunological antigens associated with orthotopic xenograft nasopharyngeal carcinoma was analyzed by tissue section analysis and immunohistochemistry (IHC). A visualized orthotopic xenograft nasopharyngeal carcinoma model was successfully developed in this study. Luminescence signal detection, micro-MRI, and hematoxylin and eosin staining revealed the successful growth of tumors in the nasopharynx of the nude mice. Moreover, IHC analysis detected cytokeratin (CK), CK5/6, P40, and P63 expression in the orthotopic tumors, consistent with the reported expression of these antigens in human nasopharyngeal tumors. This study established a reproducible, visual, and less lethal orthotopic xenograft model of nasopharyngeal carcinoma, providing a platform for preclinical research.https://www.tandfonline.com/doi/10.1080/15384047.2024.2382531Nasopharyngeal carcinomamouse tumor modelorthotopic xenograft modeltissue blockin vivo imaging |
| spellingShingle | Wei Chen Wei-Min Chen Si-Xia Chen Li Jiang Ge-Ge Shu Yuan-Xiu Yin Zhi-Peng Quan Zi-Yan Zhou Ming-Jun Shen Ya-Ting Qin Chao-Lin Yang Xue-Jin Su Min Kang Establishment of a visualized mouse orthotopic xenograft model of nasopharyngeal carcinoma Cancer Biology & Therapy Nasopharyngeal carcinoma mouse tumor model orthotopic xenograft model tissue block in vivo imaging |
| title | Establishment of a visualized mouse orthotopic xenograft model of nasopharyngeal carcinoma |
| title_full | Establishment of a visualized mouse orthotopic xenograft model of nasopharyngeal carcinoma |
| title_fullStr | Establishment of a visualized mouse orthotopic xenograft model of nasopharyngeal carcinoma |
| title_full_unstemmed | Establishment of a visualized mouse orthotopic xenograft model of nasopharyngeal carcinoma |
| title_short | Establishment of a visualized mouse orthotopic xenograft model of nasopharyngeal carcinoma |
| title_sort | establishment of a visualized mouse orthotopic xenograft model of nasopharyngeal carcinoma |
| topic | Nasopharyngeal carcinoma mouse tumor model orthotopic xenograft model tissue block in vivo imaging |
| url | https://www.tandfonline.com/doi/10.1080/15384047.2024.2382531 |
| work_keys_str_mv | AT weichen establishmentofavisualizedmouseorthotopicxenograftmodelofnasopharyngealcarcinoma AT weiminchen establishmentofavisualizedmouseorthotopicxenograftmodelofnasopharyngealcarcinoma AT sixiachen establishmentofavisualizedmouseorthotopicxenograftmodelofnasopharyngealcarcinoma AT lijiang establishmentofavisualizedmouseorthotopicxenograftmodelofnasopharyngealcarcinoma AT gegeshu establishmentofavisualizedmouseorthotopicxenograftmodelofnasopharyngealcarcinoma AT yuanxiuyin establishmentofavisualizedmouseorthotopicxenograftmodelofnasopharyngealcarcinoma AT zhipengquan establishmentofavisualizedmouseorthotopicxenograftmodelofnasopharyngealcarcinoma AT ziyanzhou establishmentofavisualizedmouseorthotopicxenograftmodelofnasopharyngealcarcinoma AT mingjunshen establishmentofavisualizedmouseorthotopicxenograftmodelofnasopharyngealcarcinoma AT yatingqin establishmentofavisualizedmouseorthotopicxenograftmodelofnasopharyngealcarcinoma AT chaolinyang establishmentofavisualizedmouseorthotopicxenograftmodelofnasopharyngealcarcinoma AT xuejinsu establishmentofavisualizedmouseorthotopicxenograftmodelofnasopharyngealcarcinoma AT minkang establishmentofavisualizedmouseorthotopicxenograftmodelofnasopharyngealcarcinoma |