Field study on the suitability of oral fluid samples for monitoring of Lawsonia intracellularis and Brachyspira hyodysenteriae by multiplex qPCR under field conditions
Abstract Background Monitoring or surveillance of infectious diseases is crucial in terms of herd health management of livestock. Investigations of oral fluids have become an animal friendly routine strategy to monitor respiratory pathogens in pigs. Less is known about the suitability of oral fluids...
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| Format: | Article |
| Language: | English |
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BMC
2025-01-01
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| Series: | Porcine Health Management |
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| Online Access: | https://doi.org/10.1186/s40813-024-00415-4 |
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| author | Matthias Eddicks Gerald Reiner Sigena Junker Hermann Willems Sabrina Becker Julia Stadler Josefine Hagn Mathias Ritzmann |
| author_facet | Matthias Eddicks Gerald Reiner Sigena Junker Hermann Willems Sabrina Becker Julia Stadler Josefine Hagn Mathias Ritzmann |
| author_sort | Matthias Eddicks |
| collection | DOAJ |
| description | Abstract Background Monitoring or surveillance of infectious diseases is crucial in terms of herd health management of livestock. Investigations of oral fluids have become an animal friendly routine strategy to monitor respiratory pathogens in pigs. Less is known about the suitability of oral fluids for the detection of enteric pathogens in swine. In the present study we evaluated the use of oral fluids to monitor B. hyodysenteriae and L. intracellularis compared to pooled fecal samples by multiplex qPCR in a pen-wise follow-up of fattening pigs. Therefore, we collected oral fluids at an age of 12, 16 and 20 weeks of life and compared them to pooled fecal samples collected from the same pens on two fattening farms. Results Cohen´s Kappa analysis revealed a substantial agreement between oral fluids and pooled fecal samples on pen level (Cohen´s Kappa: 0.745; p < 0.001). DNA-loads of L. intracellularis were tendentially higher (p = 0.053) in pooled fecal samples than in the corresponding OFs. Conclusions The present study shows that oral fluids are an appropriate tool to monitor B. hyodysenteriae and L. intracellularis on conventional fattening farms under field conditions. However, multiple pen testing should be conducted to increase the diagnostic performance and sensitivity. |
| format | Article |
| id | doaj-art-d877a8a79ef84ac9bc6fa722b6ff4bc5 |
| institution | Kabale University |
| issn | 2055-5660 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Porcine Health Management |
| spelling | doaj-art-d877a8a79ef84ac9bc6fa722b6ff4bc52025-01-12T12:44:55ZengBMCPorcine Health Management2055-56602025-01-011111910.1186/s40813-024-00415-4Field study on the suitability of oral fluid samples for monitoring of Lawsonia intracellularis and Brachyspira hyodysenteriae by multiplex qPCR under field conditionsMatthias Eddicks0Gerald Reiner1Sigena Junker2Hermann Willems3Sabrina Becker4Julia Stadler5Josefine Hagn6Mathias Ritzmann7Clinic for Swine at the Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-Universität MünchenClinic for Swine of the Justus-Liebig-Universität GießenClinic for Swine at the Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-Universität MünchenClinic for Swine of the Justus-Liebig-Universität GießenClinic for Swine of the Justus-Liebig-Universität GießenClinic for Swine at the Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-Universität MünchenVeterinary Practice HagnClinic for Swine at the Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-Universität MünchenAbstract Background Monitoring or surveillance of infectious diseases is crucial in terms of herd health management of livestock. Investigations of oral fluids have become an animal friendly routine strategy to monitor respiratory pathogens in pigs. Less is known about the suitability of oral fluids for the detection of enteric pathogens in swine. In the present study we evaluated the use of oral fluids to monitor B. hyodysenteriae and L. intracellularis compared to pooled fecal samples by multiplex qPCR in a pen-wise follow-up of fattening pigs. Therefore, we collected oral fluids at an age of 12, 16 and 20 weeks of life and compared them to pooled fecal samples collected from the same pens on two fattening farms. Results Cohen´s Kappa analysis revealed a substantial agreement between oral fluids and pooled fecal samples on pen level (Cohen´s Kappa: 0.745; p < 0.001). DNA-loads of L. intracellularis were tendentially higher (p = 0.053) in pooled fecal samples than in the corresponding OFs. Conclusions The present study shows that oral fluids are an appropriate tool to monitor B. hyodysenteriae and L. intracellularis on conventional fattening farms under field conditions. However, multiple pen testing should be conducted to increase the diagnostic performance and sensitivity.https://doi.org/10.1186/s40813-024-00415-4ScreeningEnteric diseasesSwineFatteningLivestock |
| spellingShingle | Matthias Eddicks Gerald Reiner Sigena Junker Hermann Willems Sabrina Becker Julia Stadler Josefine Hagn Mathias Ritzmann Field study on the suitability of oral fluid samples for monitoring of Lawsonia intracellularis and Brachyspira hyodysenteriae by multiplex qPCR under field conditions Porcine Health Management Screening Enteric diseases Swine Fattening Livestock |
| title | Field study on the suitability of oral fluid samples for monitoring of Lawsonia intracellularis and Brachyspira hyodysenteriae by multiplex qPCR under field conditions |
| title_full | Field study on the suitability of oral fluid samples for monitoring of Lawsonia intracellularis and Brachyspira hyodysenteriae by multiplex qPCR under field conditions |
| title_fullStr | Field study on the suitability of oral fluid samples for monitoring of Lawsonia intracellularis and Brachyspira hyodysenteriae by multiplex qPCR under field conditions |
| title_full_unstemmed | Field study on the suitability of oral fluid samples for monitoring of Lawsonia intracellularis and Brachyspira hyodysenteriae by multiplex qPCR under field conditions |
| title_short | Field study on the suitability of oral fluid samples for monitoring of Lawsonia intracellularis and Brachyspira hyodysenteriae by multiplex qPCR under field conditions |
| title_sort | field study on the suitability of oral fluid samples for monitoring of lawsonia intracellularis and brachyspira hyodysenteriae by multiplex qpcr under field conditions |
| topic | Screening Enteric diseases Swine Fattening Livestock |
| url | https://doi.org/10.1186/s40813-024-00415-4 |
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