Characterizing the hard and soft nanoparticle-protein corona with multilayer adsorption
Nanoparticles (NPs) in contact with biological fluid adsorb biomolecules into a corona. This corona comprises proteins that strongly bind to the NP (hard corona) and loosely bound proteins (soft corona) that dynamically exchange with the surrounding solution. While the kinetics of hard corona format...
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| Language: | English |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Nanotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnano.2024.1531039/full |
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| author | Oriol Vilanova Oriol Vilanova Alberto Martinez-Serra Marco P. Monopoli Giancarlo Franzese Giancarlo Franzese |
| author_facet | Oriol Vilanova Oriol Vilanova Alberto Martinez-Serra Marco P. Monopoli Giancarlo Franzese Giancarlo Franzese |
| author_sort | Oriol Vilanova |
| collection | DOAJ |
| description | Nanoparticles (NPs) in contact with biological fluid adsorb biomolecules into a corona. This corona comprises proteins that strongly bind to the NP (hard corona) and loosely bound proteins (soft corona) that dynamically exchange with the surrounding solution. While the kinetics of hard corona formation is relatively well understood, thanks to experiments and robust simulation models, the experimental characterization and simulation of the soft corona present a more significant challenge. Here, we review the current state of the art in soft corona characterization and introduce a novel open-source computational model to simulate its dynamic behavior, for which we provide the documentation. We focus on the case of transferrin (Tf) interacting with polystyrene NPs as an illustrative example, demonstrating how this model captures the complexities of the soft corona and offers deeper insights into its structure and behavior. We show that the soft corona is dominated by a glassy evolution that we relate to crowding effects. This work advances our understanding of the soft corona, bridging experimental limitations with improved simulation techniques. |
| format | Article |
| id | doaj-art-d8581fbf9cf04d608d47df5516148f97 |
| institution | Kabale University |
| issn | 2673-3013 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nanotechnology |
| spelling | doaj-art-d8581fbf9cf04d608d47df5516148f972025-01-17T05:10:06ZengFrontiers Media S.A.Frontiers in Nanotechnology2673-30132025-01-01610.3389/fnano.2024.15310391531039Characterizing the hard and soft nanoparticle-protein corona with multilayer adsorptionOriol Vilanova0Oriol Vilanova1Alberto Martinez-Serra2Marco P. Monopoli3Giancarlo Franzese4Giancarlo Franzese5Secció de Física Estadística i Interdisciplinària, Departament de Física de la Matèria Condensada, Universitat de Barcelona, Barcelona, SpainInstitut de Nanociència i Nanotecnologia, Universitat de Barcelona, Barcelona, SpainChemistry Department, Royal College of Surgeons in Ireland (RCSI), Dublin, IrelandChemistry Department, Royal College of Surgeons in Ireland (RCSI), Dublin, IrelandSecció de Física Estadística i Interdisciplinària, Departament de Física de la Matèria Condensada, Universitat de Barcelona, Barcelona, SpainInstitut de Nanociència i Nanotecnologia, Universitat de Barcelona, Barcelona, SpainNanoparticles (NPs) in contact with biological fluid adsorb biomolecules into a corona. This corona comprises proteins that strongly bind to the NP (hard corona) and loosely bound proteins (soft corona) that dynamically exchange with the surrounding solution. While the kinetics of hard corona formation is relatively well understood, thanks to experiments and robust simulation models, the experimental characterization and simulation of the soft corona present a more significant challenge. Here, we review the current state of the art in soft corona characterization and introduce a novel open-source computational model to simulate its dynamic behavior, for which we provide the documentation. We focus on the case of transferrin (Tf) interacting with polystyrene NPs as an illustrative example, demonstrating how this model captures the complexities of the soft corona and offers deeper insights into its structure and behavior. We show that the soft corona is dominated by a glassy evolution that we relate to crowding effects. This work advances our understanding of the soft corona, bridging experimental limitations with improved simulation techniques.https://www.frontiersin.org/articles/10.3389/fnano.2024.1531039/fullbiomolecular coronaprotein adsorption kineticsprotein-nanoparticle interactionscoarse-grain modelingmolecular simulationstransferrin |
| spellingShingle | Oriol Vilanova Oriol Vilanova Alberto Martinez-Serra Marco P. Monopoli Giancarlo Franzese Giancarlo Franzese Characterizing the hard and soft nanoparticle-protein corona with multilayer adsorption Frontiers in Nanotechnology biomolecular corona protein adsorption kinetics protein-nanoparticle interactions coarse-grain modeling molecular simulations transferrin |
| title | Characterizing the hard and soft nanoparticle-protein corona with multilayer adsorption |
| title_full | Characterizing the hard and soft nanoparticle-protein corona with multilayer adsorption |
| title_fullStr | Characterizing the hard and soft nanoparticle-protein corona with multilayer adsorption |
| title_full_unstemmed | Characterizing the hard and soft nanoparticle-protein corona with multilayer adsorption |
| title_short | Characterizing the hard and soft nanoparticle-protein corona with multilayer adsorption |
| title_sort | characterizing the hard and soft nanoparticle protein corona with multilayer adsorption |
| topic | biomolecular corona protein adsorption kinetics protein-nanoparticle interactions coarse-grain modeling molecular simulations transferrin |
| url | https://www.frontiersin.org/articles/10.3389/fnano.2024.1531039/full |
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