The inhibitory effect of GsMTx4 on pain sensation during tooth movement in rats

Objective To investigate the inhibitory effect of GsMTx4 on pain sensation in rats during tooth movement, open field experiments and immunohistochemical staining by conducting. Methods Fifty-eight male SD rats aged 5-6 weeks were selected and a right maxillary tooth movement model was constructed. T...

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Main Author: WANG Ruofei, SHAO Lixin, LIU Xiaotong, ZHANG Miaomiao
Format: Article
Language:zho
Published: Editorial Office of Stomatology 2024-11-01
Series:Kouqiang yixue
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Online Access:https://www.stomatology.cn/fileup/1003-9872/PDF/1731916758703-898605997.pdf
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author WANG Ruofei, SHAO Lixin, LIU Xiaotong, ZHANG Miaomiao
author_facet WANG Ruofei, SHAO Lixin, LIU Xiaotong, ZHANG Miaomiao
author_sort WANG Ruofei, SHAO Lixin, LIU Xiaotong, ZHANG Miaomiao
collection DOAJ
description Objective To investigate the inhibitory effect of GsMTx4 on pain sensation in rats during tooth movement, open field experiments and immunohistochemical staining by conducting. Methods Fifty-eight male SD rats aged 5-6 weeks were selected and a right maxillary tooth movement model was constructed. The rats were randomly divided into two groups: group A(control group) and group B(GsMTx4 group). They were also divided into 5 subgroups according to 4 h, 1 d, 3 d, 5 d, and 7 d. The left maxillary of the rats in group A was used as group C(blank control group). The open-field test was used to evaluate the pain level of rats. Immunohistochemical staining was used to observe the expression of nerve growth factor(NGF) and calcitonin gene-related peptide(CGRP) in periodontal tissues. Results At 4 h, 1 d, and 3 d, the total distance traveled by rats in group B within 5 minutes in the open-field was higher than that of group A (P<0.05). At 4 h and 1 d, the percentage of time in the center of rats in group B was higher than that in group A(P<0.05). The expressions of NGF and CGRP in groups A and B were higher than those in group C on 1 d, 3 d, 5 d, and 7 d(P<0.05). The expression of NGF and CGRP in group B were lower than those in group A at 4 h, 1 d, and 3 d(P<0.05). Conclusion GsMTx4 can inhibit the expression of NGF and CGRP during tooth movement and relieve the pain caused by tooth movement.
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spelling doaj-art-d849d538971d4c98880eb8bc5c9c81702024-11-25T00:56:52ZzhoEditorial Office of StomatologyKouqiang yixue1003-98722024-11-01441181581910.13591/j.cnki.kqyx.2024.11.003The inhibitory effect of GsMTx4 on pain sensation during tooth movement in ratsWANG Ruofei, SHAO Lixin, LIU Xiaotong, ZHANG Miaomiao0Department of Orthodontics, First Affilated Hospital of Harbin Medical University, Harbin 150001, ChinaObjective To investigate the inhibitory effect of GsMTx4 on pain sensation in rats during tooth movement, open field experiments and immunohistochemical staining by conducting. Methods Fifty-eight male SD rats aged 5-6 weeks were selected and a right maxillary tooth movement model was constructed. The rats were randomly divided into two groups: group A(control group) and group B(GsMTx4 group). They were also divided into 5 subgroups according to 4 h, 1 d, 3 d, 5 d, and 7 d. The left maxillary of the rats in group A was used as group C(blank control group). The open-field test was used to evaluate the pain level of rats. Immunohistochemical staining was used to observe the expression of nerve growth factor(NGF) and calcitonin gene-related peptide(CGRP) in periodontal tissues. Results At 4 h, 1 d, and 3 d, the total distance traveled by rats in group B within 5 minutes in the open-field was higher than that of group A (P<0.05). At 4 h and 1 d, the percentage of time in the center of rats in group B was higher than that in group A(P<0.05). The expressions of NGF and CGRP in groups A and B were higher than those in group C on 1 d, 3 d, 5 d, and 7 d(P<0.05). The expression of NGF and CGRP in group B were lower than those in group A at 4 h, 1 d, and 3 d(P<0.05). Conclusion GsMTx4 can inhibit the expression of NGF and CGRP during tooth movement and relieve the pain caused by tooth movement.https://www.stomatology.cn/fileup/1003-9872/PDF/1731916758703-898605997.pdf|gsmtx4|orthodontic|pain|ngf|cgrp
spellingShingle WANG Ruofei, SHAO Lixin, LIU Xiaotong, ZHANG Miaomiao
The inhibitory effect of GsMTx4 on pain sensation during tooth movement in rats
Kouqiang yixue
|gsmtx4|orthodontic|pain|ngf|cgrp
title The inhibitory effect of GsMTx4 on pain sensation during tooth movement in rats
title_full The inhibitory effect of GsMTx4 on pain sensation during tooth movement in rats
title_fullStr The inhibitory effect of GsMTx4 on pain sensation during tooth movement in rats
title_full_unstemmed The inhibitory effect of GsMTx4 on pain sensation during tooth movement in rats
title_short The inhibitory effect of GsMTx4 on pain sensation during tooth movement in rats
title_sort inhibitory effect of gsmtx4 on pain sensation during tooth movement in rats
topic |gsmtx4|orthodontic|pain|ngf|cgrp
url https://www.stomatology.cn/fileup/1003-9872/PDF/1731916758703-898605997.pdf
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