Synergistic Effects of Coenzyme Q10 & L-Carnitine on Oligodendrocyte Necrosis and Myelination, in a Rat Model of Multiple Sclerosis
Objective: Determining the synergistic effect of Coenzyme Q10 & L-Carnitine on Oligodendrocyte necrosis and Myelination, in a rat model of Multiple sclerosis. Study Design: Laboratory based experimental study. Place and Duration of Study: The study was conducted at the Department of Anatomy, Isl...
Saved in:
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
National University of Medical Sciences
2024-11-01
|
Series: | Life and Science |
Subjects: | |
Online Access: | https://ojs.lifenscience.org/index.php/life-and-science/article/view/547 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
_version_ | 1846150853987139584 |
---|---|
author | Tayyaba Qureshi Shabana Ali Ayesha Shahid Huma Beenish Tooba Khurshid Tayyaba Fahad |
author_facet | Tayyaba Qureshi Shabana Ali Ayesha Shahid Huma Beenish Tooba Khurshid Tayyaba Fahad |
author_sort | Tayyaba Qureshi |
collection | DOAJ |
description | Objective: Determining the synergistic effect of Coenzyme Q10 & L-Carnitine on Oligodendrocyte necrosis and
Myelination, in a rat model of Multiple sclerosis.
Study Design: Laboratory based experimental study.
Place and Duration of Study: The study was conducted at the Department of Anatomy, Islamic International
Medical College Rawalpindi, Pakistan from March 2022 to May 2022 in collaboration with NIH Islamabad, for a
duration of 12 weeks.
Methods: A total of fifty male Sprague Dawley rats were divided into five randomized groups, each with a
distinct treatment plan. While Group 1 received a standard diet, the remaining four groups were induced with
Multiple Sclerosis and administered 0.2% Cuprizone (CPZ) over a period of 12 weeks. After four weeks, Group 3
was given 150 mg/kg/day of Coenzyme Q10/Ubiquinone (CoQ10), Group 4 received 100 mg/kg/day of LCarnitine (L-Car), and Group 5 was treated with a combination of both, all while still receiving CPZ. Upon
completion of the 12-week protocol, the rats were sacrificed, and their brains were extracted. H & E staining
was performed on coronal sections to assess any changes in oligodendrocyte necrosis, while Luxol Fast Blue
(LFB) staining was utilized to visualize alterations in myelination.
Results: The combination of CoQ10 and L-Car was significantly better than the single agents in controlling the
oligodendrocyte necrosis and controlling vacuolation of myelin, as evidenced by ANOVA and F-test.
Conclusion: This study has unequivocally demonstrated that taking CoQ10 and L-Car together has a greater
effect on promoting myelination and preventing oligodendrocyte necrosis compared to using them
individually. Therefore, it is highly recommended to prescribe both medications simultaneously for those with
multiple sclerosis, as it can potentially provide greater advantages for patients.
How to cite this: Qureshi T, Ali S, Shahid A, Beenish H, Khurshid T, Fahad T. Synergistic Effects of Coenzyme Q10 & L-Carnitine on Oligodendrocyte Necrosis and Myelination, In A Rat Model of Multiple Sclerosis. Life and Science. 2024; 5(4): 471-476. doi: http://doi.org/10.37185/LnS.1.1.547 |
format | Article |
id | doaj-art-d80eaf642b4843b3b773a584a3a4bd7c |
institution | Kabale University |
issn | 2521-0475 2708-2970 |
language | English |
publishDate | 2024-11-01 |
publisher | National University of Medical Sciences |
record_format | Article |
series | Life and Science |
spelling | doaj-art-d80eaf642b4843b3b773a584a3a4bd7c2024-11-28T07:37:58ZengNational University of Medical SciencesLife and Science2521-04752708-29702024-11-0154060610.37185/LnS.1.1.547547Synergistic Effects of Coenzyme Q10 & L-Carnitine on Oligodendrocyte Necrosis and Myelination, in a Rat Model of Multiple SclerosisTayyaba Qureshi0Shabana Ali1Ayesha Shahid2Huma Beenish3Tooba Khurshid4Tayyaba Fahad5Rawalpindi Medical University Rawalpindi, PakistanIslamic International Medical College, Al-Mizan Campus Rawalpindi, PakistanHITEC Institute of Medical Sciences Taxila, PakistanNUST School of Health Sciences Islamabad, PakistanArmy Medical College Rawalpindi, PakistanBahria University College of Medicine, Naval Anchorage Islamabad, PakistanObjective: Determining the synergistic effect of Coenzyme Q10 & L-Carnitine on Oligodendrocyte necrosis and Myelination, in a rat model of Multiple sclerosis. Study Design: Laboratory based experimental study. Place and Duration of Study: The study was conducted at the Department of Anatomy, Islamic International Medical College Rawalpindi, Pakistan from March 2022 to May 2022 in collaboration with NIH Islamabad, for a duration of 12 weeks. Methods: A total of fifty male Sprague Dawley rats were divided into five randomized groups, each with a distinct treatment plan. While Group 1 received a standard diet, the remaining four groups were induced with Multiple Sclerosis and administered 0.2% Cuprizone (CPZ) over a period of 12 weeks. After four weeks, Group 3 was given 150 mg/kg/day of Coenzyme Q10/Ubiquinone (CoQ10), Group 4 received 100 mg/kg/day of LCarnitine (L-Car), and Group 5 was treated with a combination of both, all while still receiving CPZ. Upon completion of the 12-week protocol, the rats were sacrificed, and their brains were extracted. H & E staining was performed on coronal sections to assess any changes in oligodendrocyte necrosis, while Luxol Fast Blue (LFB) staining was utilized to visualize alterations in myelination. Results: The combination of CoQ10 and L-Car was significantly better than the single agents in controlling the oligodendrocyte necrosis and controlling vacuolation of myelin, as evidenced by ANOVA and F-test. Conclusion: This study has unequivocally demonstrated that taking CoQ10 and L-Car together has a greater effect on promoting myelination and preventing oligodendrocyte necrosis compared to using them individually. Therefore, it is highly recommended to prescribe both medications simultaneously for those with multiple sclerosis, as it can potentially provide greater advantages for patients. How to cite this: Qureshi T, Ali S, Shahid A, Beenish H, Khurshid T, Fahad T. Synergistic Effects of Coenzyme Q10 & L-Carnitine on Oligodendrocyte Necrosis and Myelination, In A Rat Model of Multiple Sclerosis. Life and Science. 2024; 5(4): 471-476. doi: http://doi.org/10.37185/LnS.1.1.547https://ojs.lifenscience.org/index.php/life-and-science/article/view/547l-carnitine, multiple sclerosis, oligodendrocyte, ubiquinone. |
spellingShingle | Tayyaba Qureshi Shabana Ali Ayesha Shahid Huma Beenish Tooba Khurshid Tayyaba Fahad Synergistic Effects of Coenzyme Q10 & L-Carnitine on Oligodendrocyte Necrosis and Myelination, in a Rat Model of Multiple Sclerosis Life and Science l-carnitine, multiple sclerosis, oligodendrocyte, ubiquinone. |
title | Synergistic Effects of Coenzyme Q10 & L-Carnitine on Oligodendrocyte Necrosis and Myelination, in a Rat Model of Multiple Sclerosis |
title_full | Synergistic Effects of Coenzyme Q10 & L-Carnitine on Oligodendrocyte Necrosis and Myelination, in a Rat Model of Multiple Sclerosis |
title_fullStr | Synergistic Effects of Coenzyme Q10 & L-Carnitine on Oligodendrocyte Necrosis and Myelination, in a Rat Model of Multiple Sclerosis |
title_full_unstemmed | Synergistic Effects of Coenzyme Q10 & L-Carnitine on Oligodendrocyte Necrosis and Myelination, in a Rat Model of Multiple Sclerosis |
title_short | Synergistic Effects of Coenzyme Q10 & L-Carnitine on Oligodendrocyte Necrosis and Myelination, in a Rat Model of Multiple Sclerosis |
title_sort | synergistic effects of coenzyme q10 l carnitine on oligodendrocyte necrosis and myelination in a rat model of multiple sclerosis |
topic | l-carnitine, multiple sclerosis, oligodendrocyte, ubiquinone. |
url | https://ojs.lifenscience.org/index.php/life-and-science/article/view/547 |
work_keys_str_mv | AT tayyabaqureshi synergisticeffectsofcoenzymeq10lcarnitineonoligodendrocytenecrosisandmyelinationinaratmodelofmultiplesclerosis AT shabanaali synergisticeffectsofcoenzymeq10lcarnitineonoligodendrocytenecrosisandmyelinationinaratmodelofmultiplesclerosis AT ayeshashahid synergisticeffectsofcoenzymeq10lcarnitineonoligodendrocytenecrosisandmyelinationinaratmodelofmultiplesclerosis AT humabeenish synergisticeffectsofcoenzymeq10lcarnitineonoligodendrocytenecrosisandmyelinationinaratmodelofmultiplesclerosis AT toobakhurshid synergisticeffectsofcoenzymeq10lcarnitineonoligodendrocytenecrosisandmyelinationinaratmodelofmultiplesclerosis AT tayyabafahad synergisticeffectsofcoenzymeq10lcarnitineonoligodendrocytenecrosisandmyelinationinaratmodelofmultiplesclerosis |