Adsorption of Cellulase Isolated from on Chitosan/Alginate Particles Functionalized with Epichlorohydrin
This work investigates the adsorption of cellulase (isolated from Aspergillus niger ) in a fixed-bed column using chitosan/alginate particles functionalized with epichlorohydrin (Cs/Ag–E) as the adsorbent. The particles were prepared and characterized with respect to apparent density, particle poros...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2013-02-01
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| Series: | Adsorption Science & Technology |
| Online Access: | https://doi.org/10.1260/0263-6174.31.1.17 |
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| author | Elaine C. Rodrigues Bruna T. C. Bezerra Barbara V. Farias Wellington S. Adriano Rodrigo S. Vieira Diana C. S. Azevedo Ivanildo J. Silva |
| author_facet | Elaine C. Rodrigues Bruna T. C. Bezerra Barbara V. Farias Wellington S. Adriano Rodrigo S. Vieira Diana C. S. Azevedo Ivanildo J. Silva |
| author_sort | Elaine C. Rodrigues |
| collection | DOAJ |
| description | This work investigates the adsorption of cellulase (isolated from Aspergillus niger ) in a fixed-bed column using chitosan/alginate particles functionalized with epichlorohydrin (Cs/Ag–E) as the adsorbent. The particles were prepared and characterized with respect to apparent density, particle porosity, particle size, surface chemistry and thermal stability. Cellulase solutions were prepared in sodium citrate buffer (20 mM) at pH 5. Several breakthrough curves were obtained by varying the flow rate and feed concentrations in order to evaluate the suitable bed height and determine the adsorption isotherm, respectively. The adsorption isotherm fitted well with Langmuir equation with maximum adsorption capacity of about 11 mg of cellulase/g of adsorbent. Successive cycles (adsorption/washing/elution) were performed and the results demonstrate the reversibility of the process and its potential for enzyme purification and separation. However, cellulase activity in the eluted fraction is only 29% of that of the fresh enzyme fed to the column. |
| format | Article |
| id | doaj-art-d801e93abea744908f81b6e71b49e439 |
| institution | Kabale University |
| issn | 0263-6174 2048-4038 |
| language | English |
| publishDate | 2013-02-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Adsorption Science & Technology |
| spelling | doaj-art-d801e93abea744908f81b6e71b49e4392025-01-02T22:39:32ZengSAGE PublishingAdsorption Science & Technology0263-61742048-40382013-02-013110.1260/0263-6174.31.1.17Adsorption of Cellulase Isolated from on Chitosan/Alginate Particles Functionalized with EpichlorohydrinElaine C. Rodrigues0Bruna T. C. Bezerra1Barbara V. Farias2Wellington S. Adriano3Rodrigo S. Vieira4Diana C. S. Azevedo5Ivanildo J. Silva6 Chemical Engineering Department, Federal University of Ceará, Grupo de Pesquisa em Separações por Adsorção - GPSA, Campus do Pici, Bl. 709, CEP 60455-760, Fortaleza - CE - Brazil Chemical Engineering Department, Federal University of Ceará, Grupo de Pesquisa em Separações por Adsorção - GPSA, Campus do Pici, Bl. 709, CEP 60455-760, Fortaleza - CE - Brazil Chemical Engineering Department, Federal University of Ceará, Grupo de Pesquisa em Separações por Adsorção - GPSA, Campus do Pici, Bl. 709, CEP 60455-760, Fortaleza - CE - Brazil Laboratory of Pharmaceutical Science - CES/UAS, Campina Grande Federal University, 58175-000, Cuite - PB - Brazil Chemical Engineering Department, Federal University of Ceará, Grupo de Pesquisa em Separações por Adsorção - GPSA, Campus do Pici, Bl. 709, CEP 60455-760, Fortaleza - CE - Brazil Chemical Engineering Department, Federal University of Ceará, Grupo de Pesquisa em Separações por Adsorção - GPSA, Campus do Pici, Bl. 709, CEP 60455-760, Fortaleza - CE - Brazil Chemical Engineering Department, Federal University of Ceará, Grupo de Pesquisa em Separações por Adsorção - GPSA, Campus do Pici, Bl. 709, CEP 60455-760, Fortaleza - CE - BrazilThis work investigates the adsorption of cellulase (isolated from Aspergillus niger ) in a fixed-bed column using chitosan/alginate particles functionalized with epichlorohydrin (Cs/Ag–E) as the adsorbent. The particles were prepared and characterized with respect to apparent density, particle porosity, particle size, surface chemistry and thermal stability. Cellulase solutions were prepared in sodium citrate buffer (20 mM) at pH 5. Several breakthrough curves were obtained by varying the flow rate and feed concentrations in order to evaluate the suitable bed height and determine the adsorption isotherm, respectively. The adsorption isotherm fitted well with Langmuir equation with maximum adsorption capacity of about 11 mg of cellulase/g of adsorbent. Successive cycles (adsorption/washing/elution) were performed and the results demonstrate the reversibility of the process and its potential for enzyme purification and separation. However, cellulase activity in the eluted fraction is only 29% of that of the fresh enzyme fed to the column.https://doi.org/10.1260/0263-6174.31.1.17 |
| spellingShingle | Elaine C. Rodrigues Bruna T. C. Bezerra Barbara V. Farias Wellington S. Adriano Rodrigo S. Vieira Diana C. S. Azevedo Ivanildo J. Silva Adsorption of Cellulase Isolated from on Chitosan/Alginate Particles Functionalized with Epichlorohydrin Adsorption Science & Technology |
| title | Adsorption of Cellulase Isolated from on Chitosan/Alginate Particles Functionalized with Epichlorohydrin |
| title_full | Adsorption of Cellulase Isolated from on Chitosan/Alginate Particles Functionalized with Epichlorohydrin |
| title_fullStr | Adsorption of Cellulase Isolated from on Chitosan/Alginate Particles Functionalized with Epichlorohydrin |
| title_full_unstemmed | Adsorption of Cellulase Isolated from on Chitosan/Alginate Particles Functionalized with Epichlorohydrin |
| title_short | Adsorption of Cellulase Isolated from on Chitosan/Alginate Particles Functionalized with Epichlorohydrin |
| title_sort | adsorption of cellulase isolated from on chitosan alginate particles functionalized with epichlorohydrin |
| url | https://doi.org/10.1260/0263-6174.31.1.17 |
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