Rooperol, an inhibitor of cytokine synthesis, decreases the respiratory burst in human and rat leukocytes and macrophages
Luminol-enhanced chemiluminescence was measured in fresh whole human blood, or human neutrophils isolated from heparinized blood, human alveolar macrophages and rat alveolar macrophages stimulated with bacterial endotoxin (LPS). Tetraacetate esters of rooperol, a dicatechol showing anticytokine acti...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
1997-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1080/09629359791938 |
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| _version_ | 1841524494762508288 |
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| author | A. Guzdek B. Turyna A. C. Allison K. Sladek A. Koj |
| author_facet | A. Guzdek B. Turyna A. C. Allison K. Sladek A. Koj |
| author_sort | A. Guzdek |
| collection | DOAJ |
| description | Luminol-enhanced chemiluminescence was measured in fresh whole human blood, or human neutrophils isolated from heparinized blood, human alveolar macrophages and rat alveolar macrophages stimulated with bacterial endotoxin (LPS). Tetraacetate esters of rooperol, a dicatechol showing anticytokine activity, added to cells simultaneously with LPS inhibited the respiratory burst. The effective concentrations of rooperol were in the range of 1-10 μM depending on cell type and corresponded well with inhibition of nitric oxide production by rat alveolar macrophages. Thus rooperol may reduce some effects of excessive phagocytic activity and inflammatory reaction but by quenching free radicals production may also diminish the resistance to bacterial infections. |
| format | Article |
| id | doaj-art-d7fb4889b58446408a31b599d6823ec4 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1997-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-d7fb4889b58446408a31b599d6823ec42025-02-03T05:53:13ZengWileyMediators of Inflammation0962-93511466-18611997-01-0161535710.1080/09629359791938Rooperol, an inhibitor of cytokine synthesis, decreases the respiratory burst in human and rat leukocytes and macrophagesA. Guzdek0B. Turyna1A. C. Allison2K. Sladek3A. Koj4Institute of Molecular Biology, Jagiellonian University, Cracow 31-120, PolandInstitute of Molecular Biology, Jagiellonian University, Cracow 31-120, PolandDAWA Inc., Belmont, CA 94002, USAInstitute of Internal Medicine, Collegium Medicum, Jagiellonian University, Cracow 31-066, PolandInstitute of Molecular Biology, Jagiellonian University, Cracow 31-120, PolandLuminol-enhanced chemiluminescence was measured in fresh whole human blood, or human neutrophils isolated from heparinized blood, human alveolar macrophages and rat alveolar macrophages stimulated with bacterial endotoxin (LPS). Tetraacetate esters of rooperol, a dicatechol showing anticytokine activity, added to cells simultaneously with LPS inhibited the respiratory burst. The effective concentrations of rooperol were in the range of 1-10 μM depending on cell type and corresponded well with inhibition of nitric oxide production by rat alveolar macrophages. Thus rooperol may reduce some effects of excessive phagocytic activity and inflammatory reaction but by quenching free radicals production may also diminish the resistance to bacterial infections.http://dx.doi.org/10.1080/09629359791938 |
| spellingShingle | A. Guzdek B. Turyna A. C. Allison K. Sladek A. Koj Rooperol, an inhibitor of cytokine synthesis, decreases the respiratory burst in human and rat leukocytes and macrophages Mediators of Inflammation |
| title | Rooperol, an inhibitor of cytokine synthesis, decreases the respiratory burst in human and rat leukocytes and macrophages |
| title_full | Rooperol, an inhibitor of cytokine synthesis, decreases the respiratory burst in human and rat leukocytes and macrophages |
| title_fullStr | Rooperol, an inhibitor of cytokine synthesis, decreases the respiratory burst in human and rat leukocytes and macrophages |
| title_full_unstemmed | Rooperol, an inhibitor of cytokine synthesis, decreases the respiratory burst in human and rat leukocytes and macrophages |
| title_short | Rooperol, an inhibitor of cytokine synthesis, decreases the respiratory burst in human and rat leukocytes and macrophages |
| title_sort | rooperol an inhibitor of cytokine synthesis decreases the respiratory burst in human and rat leukocytes and macrophages |
| url | http://dx.doi.org/10.1080/09629359791938 |
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