Btr02, a TRIM39 gene is related to the hypoxia tolerance of Megalobrama amblycephala
Megalobrama amblycephala is susceptible to hypoxia, which results high mortality rates in aquaculture. A preliminary study on a new variety of M. amblycephala called ''Pujiang No.2'' (GS-01–002–2020) discovered that its tolerance to hypoxia was significantly improved compared wit...
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| Language: | English |
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Elsevier
2024-12-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424006185 |
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| author | Shan-Shan Zhao Guo-Dong Zheng Shu-Ming Zou |
| author_facet | Shan-Shan Zhao Guo-Dong Zheng Shu-Ming Zou |
| author_sort | Shan-Shan Zhao |
| collection | DOAJ |
| description | Megalobrama amblycephala is susceptible to hypoxia, which results high mortality rates in aquaculture. A preliminary study on a new variety of M. amblycephala called ''Pujiang No.2'' (GS-01–002–2020) discovered that its tolerance to hypoxia was significantly improved compared with that of wild breed. The objective of this study was to identify the key genes responsible for the enhanced hypoxia tolerance of this new variety. Based on previous RNA sequencing results, the function of btr02 (bloodthirsty-like tripartite motif [TRIM] 02 gene), a key gene for hypoxia tolerance, was explored using CRISPR/Cas9. The results demonstrated that the mutation rate of M. amblycephala btr02+/- was 39.38 %. The 5-month-old mutant was of the same size as normal individuals; however, its tolerance to hypoxia decreased substantially. Specifically, the LOEcrit of knockout mutants increased significantly (P<0.01), and the activity of antioxidant enzymes and the number of erythrocytes decreased significantly (P<0.01) after hypoxia stress. Nevertheless, there was no significant difference in gill morphology between mutant and control groups after hypoxia. These results imply that the btr02 gene of M. amblycephala influences the ability of fish to adapt to hypoxia by affecting the antioxidant stress processes and erythrocyte production. |
| format | Article |
| id | doaj-art-d7ae7172c00546f49751b9f9ca31ccd4 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-d7ae7172c00546f49751b9f9ca31ccd42024-12-03T04:29:36ZengElsevierAquaculture Reports2352-51342024-12-0139102530Btr02, a TRIM39 gene is related to the hypoxia tolerance of Megalobrama amblycephalaShan-Shan Zhao0Guo-Dong Zheng1Shu-Ming Zou2College of Fisheries, Zhejiang Ocean University, Zhoushan, Zhejiang Province 316022, ChinaNational Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, ChinaNational Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Corresponding author.Megalobrama amblycephala is susceptible to hypoxia, which results high mortality rates in aquaculture. A preliminary study on a new variety of M. amblycephala called ''Pujiang No.2'' (GS-01–002–2020) discovered that its tolerance to hypoxia was significantly improved compared with that of wild breed. The objective of this study was to identify the key genes responsible for the enhanced hypoxia tolerance of this new variety. Based on previous RNA sequencing results, the function of btr02 (bloodthirsty-like tripartite motif [TRIM] 02 gene), a key gene for hypoxia tolerance, was explored using CRISPR/Cas9. The results demonstrated that the mutation rate of M. amblycephala btr02+/- was 39.38 %. The 5-month-old mutant was of the same size as normal individuals; however, its tolerance to hypoxia decreased substantially. Specifically, the LOEcrit of knockout mutants increased significantly (P<0.01), and the activity of antioxidant enzymes and the number of erythrocytes decreased significantly (P<0.01) after hypoxia stress. Nevertheless, there was no significant difference in gill morphology between mutant and control groups after hypoxia. These results imply that the btr02 gene of M. amblycephala influences the ability of fish to adapt to hypoxia by affecting the antioxidant stress processes and erythrocyte production.http://www.sciencedirect.com/science/article/pii/S2352513424006185Megalobrama amblycephalaHypoxiabtr02CRISPR/Cas9TRIM39 |
| spellingShingle | Shan-Shan Zhao Guo-Dong Zheng Shu-Ming Zou Btr02, a TRIM39 gene is related to the hypoxia tolerance of Megalobrama amblycephala Aquaculture Reports Megalobrama amblycephala Hypoxia btr02 CRISPR/Cas9 TRIM39 |
| title | Btr02, a TRIM39 gene is related to the hypoxia tolerance of Megalobrama amblycephala |
| title_full | Btr02, a TRIM39 gene is related to the hypoxia tolerance of Megalobrama amblycephala |
| title_fullStr | Btr02, a TRIM39 gene is related to the hypoxia tolerance of Megalobrama amblycephala |
| title_full_unstemmed | Btr02, a TRIM39 gene is related to the hypoxia tolerance of Megalobrama amblycephala |
| title_short | Btr02, a TRIM39 gene is related to the hypoxia tolerance of Megalobrama amblycephala |
| title_sort | btr02 a trim39 gene is related to the hypoxia tolerance of megalobrama amblycephala |
| topic | Megalobrama amblycephala Hypoxia btr02 CRISPR/Cas9 TRIM39 |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424006185 |
| work_keys_str_mv | AT shanshanzhao btr02atrim39geneisrelatedtothehypoxiatoleranceofmegalobramaamblycephala AT guodongzheng btr02atrim39geneisrelatedtothehypoxiatoleranceofmegalobramaamblycephala AT shumingzou btr02atrim39geneisrelatedtothehypoxiatoleranceofmegalobramaamblycephala |