Potential effects of adenosine triphosphate and melatonin on oxidative and inflammatory optic nerve damage in rats caused by 5-fluorouracil
AIM: To investigate the effects of adenosine triphosphate (ATP) and melatonin, which have antioxidant and anti-inflammatory activities, on potential 5-fluorouracil (5-FU)-induced optic nerve damage in rats. METHODS: Twenty-four rats were categorized into four groups of six rats: healthy (HG), 5-FU (...
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Press of International Journal of Ophthalmology (IJO PRESS)
2025-02-01
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| Series: | International Journal of Ophthalmology |
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| Online Access: | http://ies.ijo.cn/en_publish/2025/2/20250204.pdf |
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| author | Ahmet Mehmet Somuncu Busra Parlak Somuncu Ahmet Duhan Ozbay Ibrahim Cicek Bahadir Suleyman Renad Mammadov Seval Bulut Tugba Bal Tastan Taha Abdulkadir Coban Halis Suleyman Aliyev Aydin |
| author_facet | Ahmet Mehmet Somuncu Busra Parlak Somuncu Ahmet Duhan Ozbay Ibrahim Cicek Bahadir Suleyman Renad Mammadov Seval Bulut Tugba Bal Tastan Taha Abdulkadir Coban Halis Suleyman Aliyev Aydin |
| author_sort | Ahmet Mehmet Somuncu |
| collection | DOAJ |
| description | AIM: To investigate the effects of adenosine triphosphate (ATP) and melatonin, which have antioxidant and anti-inflammatory activities, on potential 5-fluorouracil (5-FU)-induced optic nerve damage in rats. METHODS: Twenty-four rats were categorized into four groups of six rats: healthy (HG), 5-FU (FUG), ATP+5-FU (AFU), and melatonin+5-FU (MFU). ATP (4 mg/kg) and melatonin (10 mg/kg) were administered intraperitoneally and orally, respectively. One hour after ATP and melatonin administration, rats in the AFU, MFU, and FUG were intraperitoneally injected with 5-FU (100 mg/kg). ATP and melatonin were administered once daily for 10d. 5-FU was administered at a single dose on days 1, 3, and 5 of the experiment. After 10d, the rats were euthanized and optic nerve tissues were extracted. Optic nerve tissues were biochemically and histopathologically examined. RESULTS: ATP and melatonin treatments inhibited the increase in malondialdehyde (MDA) and interleukin-6 (IL-6) levels, which were elevated in the FUG. The treatments also prevented the decrease in total glutathione (tGSH) levels and the superoxide dismutase (SOD) and catalase (CAT) activities (P<0.001). This inhibition was higher in the ATP group than in the melatonin group (P<0.001). ATP prevented histopathological damage better than melatonin (P<0.05). CONCLUSION: ATP and melatonin have the potential to be used in alleviating 5-FU-induced optic nerve damage. In addition, ATP treatment shows better protective effects than melatonin. |
| format | Article |
| id | doaj-art-d7952deabbe84b619b3868d01bc3a724 |
| institution | Kabale University |
| issn | 2222-3959 2227-4898 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj-art-d7952deabbe84b619b3868d01bc3a7242025-01-16T07:54:35ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982025-02-0118222222810.18240/ijo.2025.02.0420250204Potential effects of adenosine triphosphate and melatonin on oxidative and inflammatory optic nerve damage in rats caused by 5-fluorouracilAhmet Mehmet Somuncu0Busra Parlak Somuncu1Ahmet Duhan Ozbay2Ibrahim Cicek3Bahadir Suleyman4Renad Mammadov5Seval Bulut6Tugba Bal Tastan7Taha Abdulkadir Coban8Halis Suleyman9Aliyev Aydin10Halis Suleyman. Department of Pharmacology, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan 24100, Türkiye. halis.suleyman@gmail.comProvincial Health Directorate, Ministry of Health of the Republic of Turkey, Trabzon 61040, TurkieyDepartment of Ophthalmology, Erzurum City Hospital, Health Sciences University, Erzurum 25240, TurkieyDepartment of Ophthalmology, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan 24100, TurkieyDepartment of Pharmacology, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan 24100, TurkieyDepartment of Pharmacology, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan 24100, TurkieyDepartment of Pharmacology, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan 24100, TurkieyDepartment of Histology and Embryology, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan 24100, TurkieyDepartment of Biochemistry, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan 24100, TurkeyDepartment of Pharmacology, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan 24100, TurkieyDepartment of Pharmacology, Azerbaijan Medical University, Baku, AZ1022, AzerbaijanAIM: To investigate the effects of adenosine triphosphate (ATP) and melatonin, which have antioxidant and anti-inflammatory activities, on potential 5-fluorouracil (5-FU)-induced optic nerve damage in rats. METHODS: Twenty-four rats were categorized into four groups of six rats: healthy (HG), 5-FU (FUG), ATP+5-FU (AFU), and melatonin+5-FU (MFU). ATP (4 mg/kg) and melatonin (10 mg/kg) were administered intraperitoneally and orally, respectively. One hour after ATP and melatonin administration, rats in the AFU, MFU, and FUG were intraperitoneally injected with 5-FU (100 mg/kg). ATP and melatonin were administered once daily for 10d. 5-FU was administered at a single dose on days 1, 3, and 5 of the experiment. After 10d, the rats were euthanized and optic nerve tissues were extracted. Optic nerve tissues were biochemically and histopathologically examined. RESULTS: ATP and melatonin treatments inhibited the increase in malondialdehyde (MDA) and interleukin-6 (IL-6) levels, which were elevated in the FUG. The treatments also prevented the decrease in total glutathione (tGSH) levels and the superoxide dismutase (SOD) and catalase (CAT) activities (P<0.001). This inhibition was higher in the ATP group than in the melatonin group (P<0.001). ATP prevented histopathological damage better than melatonin (P<0.05). CONCLUSION: ATP and melatonin have the potential to be used in alleviating 5-FU-induced optic nerve damage. In addition, ATP treatment shows better protective effects than melatonin.http://ies.ijo.cn/en_publish/2025/2/20250204.pdfadenosine triphosphatemelatonin5-fluorouraciloptic nerve damageantioxidantanti-inflammatory |
| spellingShingle | Ahmet Mehmet Somuncu Busra Parlak Somuncu Ahmet Duhan Ozbay Ibrahim Cicek Bahadir Suleyman Renad Mammadov Seval Bulut Tugba Bal Tastan Taha Abdulkadir Coban Halis Suleyman Aliyev Aydin Potential effects of adenosine triphosphate and melatonin on oxidative and inflammatory optic nerve damage in rats caused by 5-fluorouracil International Journal of Ophthalmology adenosine triphosphate melatonin 5-fluorouracil optic nerve damage antioxidant anti-inflammatory |
| title | Potential effects of adenosine triphosphate and melatonin on oxidative and inflammatory optic nerve damage in rats caused by 5-fluorouracil |
| title_full | Potential effects of adenosine triphosphate and melatonin on oxidative and inflammatory optic nerve damage in rats caused by 5-fluorouracil |
| title_fullStr | Potential effects of adenosine triphosphate and melatonin on oxidative and inflammatory optic nerve damage in rats caused by 5-fluorouracil |
| title_full_unstemmed | Potential effects of adenosine triphosphate and melatonin on oxidative and inflammatory optic nerve damage in rats caused by 5-fluorouracil |
| title_short | Potential effects of adenosine triphosphate and melatonin on oxidative and inflammatory optic nerve damage in rats caused by 5-fluorouracil |
| title_sort | potential effects of adenosine triphosphate and melatonin on oxidative and inflammatory optic nerve damage in rats caused by 5 fluorouracil |
| topic | adenosine triphosphate melatonin 5-fluorouracil optic nerve damage antioxidant anti-inflammatory |
| url | http://ies.ijo.cn/en_publish/2025/2/20250204.pdf |
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