Citrus aurantium L. extract alleviate depression by inhibiting gut microbiota-mediated inflammation in mice
Gut microbiota plays a crucial role in the pathophysiology of depression. This study aimed to explore the antidepressant effect of mature whole Citrus aurantium fruit extract (FEMC) in the chronic unpredictable mild stress (CUMS) model. The behavioral tests were applied to assess antidepressant effe...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2024-11-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2023.9250025 |
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| author | Tao Li Guirong Zeng Lingfeng Zhu Yanyang Wu Qun Zhang Fuhua Fu Donglin Su Gaoyang Li Qili Li Yang Shan |
| author_facet | Tao Li Guirong Zeng Lingfeng Zhu Yanyang Wu Qun Zhang Fuhua Fu Donglin Su Gaoyang Li Qili Li Yang Shan |
| author_sort | Tao Li |
| collection | DOAJ |
| description | Gut microbiota plays a crucial role in the pathophysiology of depression. This study aimed to explore the antidepressant effect of mature whole Citrus aurantium fruit extract (FEMC) in the chronic unpredictable mild stress (CUMS) model. The behavioral tests were applied to assess antidepressant effect and 16S rRNA sequencing was used to analyze the changes of gut microbiota. The results showed that the major components of FEMC were naringin and neohesperidin and significantly increased the sucrose preference index of the mice. FEMC also could reduce the feeding latency in an open field test and the rest time in a novelty suppressed feeding test. In addition, FEMC could increase CUMS-induced reduction in the levels of BDNF, PSD95, and SYN in the hippocampus. Moreover, FEMC intervention slightly decreased the ratio of Firmicutes to Bacteroidota. Meanwhile, FEMC reduced the abundance of the Prevotellaceae_Ga6A1_group, [Ruminococcus]_torques_group, which have been reported to be closely related to inflammation. Bioinformatics analysis revealed that mitogen-activated protein kinase (MAPK) signaling pathway and lipopolysaccharide biosynthesis were involved in the anti-inflammatory effect of FEMC in the CUMS animal model. Finally, the ELISA results showed that FEMC could significantly reduce the expression of pro-inflammatory cytokines IL-6 and TNF-α in the serum of depressive mice. Our results suggest FEMC can ameliorate depressive behavior by inhibiting gut microbiota-mediated inflammation in mice. |
| format | Article |
| id | doaj-art-d73c914a007d45d4928d01917e09e144 |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-d73c914a007d45d4928d01917e09e1442025-01-10T06:57:02ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-11-011363403341410.26599/FSHW.2023.9250025Citrus aurantium L. extract alleviate depression by inhibiting gut microbiota-mediated inflammation in miceTao Li0Guirong Zeng1Lingfeng Zhu2Yanyang Wu3Qun Zhang4Fuhua Fu5Donglin Su6Gaoyang Li7Qili Li8Yang Shan9Hunan Agricultural Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaHunan Key Laboratory of Pharmacodynamics and Safety Evaluation of New Drugs, Hunan Provincial Research Drugs Safety Evaluation Research Center, Changsha 410331, ChinaHunan Agricultural Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaCollege of Food Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaHunan Agricultural Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaHunan Agricultural Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaHunan Agricultural Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaHunan Agricultural Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaHunan Agricultural Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaHunan Agricultural Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaGut microbiota plays a crucial role in the pathophysiology of depression. This study aimed to explore the antidepressant effect of mature whole Citrus aurantium fruit extract (FEMC) in the chronic unpredictable mild stress (CUMS) model. The behavioral tests were applied to assess antidepressant effect and 16S rRNA sequencing was used to analyze the changes of gut microbiota. The results showed that the major components of FEMC were naringin and neohesperidin and significantly increased the sucrose preference index of the mice. FEMC also could reduce the feeding latency in an open field test and the rest time in a novelty suppressed feeding test. In addition, FEMC could increase CUMS-induced reduction in the levels of BDNF, PSD95, and SYN in the hippocampus. Moreover, FEMC intervention slightly decreased the ratio of Firmicutes to Bacteroidota. Meanwhile, FEMC reduced the abundance of the Prevotellaceae_Ga6A1_group, [Ruminococcus]_torques_group, which have been reported to be closely related to inflammation. Bioinformatics analysis revealed that mitogen-activated protein kinase (MAPK) signaling pathway and lipopolysaccharide biosynthesis were involved in the anti-inflammatory effect of FEMC in the CUMS animal model. Finally, the ELISA results showed that FEMC could significantly reduce the expression of pro-inflammatory cytokines IL-6 and TNF-α in the serum of depressive mice. Our results suggest FEMC can ameliorate depressive behavior by inhibiting gut microbiota-mediated inflammation in mice.https://www.sciopen.com/article/10.26599/FSHW.2023.9250025citrus aurantium l.chronic unpredictable mild stressdepressionflavonoidsgut microbiota |
| spellingShingle | Tao Li Guirong Zeng Lingfeng Zhu Yanyang Wu Qun Zhang Fuhua Fu Donglin Su Gaoyang Li Qili Li Yang Shan Citrus aurantium L. extract alleviate depression by inhibiting gut microbiota-mediated inflammation in mice Food Science and Human Wellness citrus aurantium l. chronic unpredictable mild stress depression flavonoids gut microbiota |
| title | Citrus aurantium L. extract alleviate depression by inhibiting gut microbiota-mediated inflammation in mice |
| title_full | Citrus aurantium L. extract alleviate depression by inhibiting gut microbiota-mediated inflammation in mice |
| title_fullStr | Citrus aurantium L. extract alleviate depression by inhibiting gut microbiota-mediated inflammation in mice |
| title_full_unstemmed | Citrus aurantium L. extract alleviate depression by inhibiting gut microbiota-mediated inflammation in mice |
| title_short | Citrus aurantium L. extract alleviate depression by inhibiting gut microbiota-mediated inflammation in mice |
| title_sort | citrus aurantium l extract alleviate depression by inhibiting gut microbiota mediated inflammation in mice |
| topic | citrus aurantium l. chronic unpredictable mild stress depression flavonoids gut microbiota |
| url | https://www.sciopen.com/article/10.26599/FSHW.2023.9250025 |
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