Exploration of the relationship between ACLY and esophageal cancer based on bioinformatics and related experiments
Objective This study focuses on ATP citrate lyase (ACLY), takes esophageal cancer cells and online databases as the main research subjects, and further explores the expression of ACLY in esophageal cancer cells and its effects on cell growth and proliferation through bioinformatics analysis and cell...
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| Format: | Article |
| Language: | zho |
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Editorial Office of New Medicine
2023-10-01
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| Series: | Yixue xinzhi zazhi |
| Subjects: | |
| Online Access: | https://yxxz.whuznhmedj.com/futureApi/storage/attach/2310/F7g35mN9wy0k9QizKTfyxem4viE7WklhRMp7V0Qx.pdf |
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| _version_ | 1846109529353224192 |
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| author | Meng WANG Yang-Yang ZHANG Jing LIU |
| author_facet | Meng WANG Yang-Yang ZHANG Jing LIU |
| author_sort | Meng WANG |
| collection | DOAJ |
| description | Objective This study focuses on ATP citrate lyase (ACLY), takes esophageal cancer cells and online databases as the main research subjects, and further explores the expression of ACLY in esophageal cancer cells and its effects on cell growth and proliferation through bioinformatics analysis and cell biology experiments, in order to provide a new target for clinical diagnosis and treatment of esophageal cancer.Method The databases such as TCGA were used to analyze the expression of ACLY in esophageal cancer patients, as well as the expression of ACLY in esophageal cancer patients of different race groups and different clinical stages of esophageal cancer. At the same time, the effects of ACLY changes on the proliferation and cell cycle of esophageal cancer cells were detected by basic cell experiments such as CCK-8 method, EdU staining experiment, plate cloning experiment and flow cytometry.Results ACLY is highly expressed in various tumor tissues including esophageal cancer. The expression of ACLY is universal, independent of race and gender, and its expression is related to the staging of esophageal cancer and lymph node metastasis. Further experiments in cell biology confirmed that knocking down ACLY could inhibit the proliferation of esophageal cancer cells and block the cell cycle. Overexpression of ACLY could increase the proliferative capacity of esophageal cancer cells and promote cell cycle progression.Conclusion ACLY can affect the proliferation ability of esophageal cancer cells, and ACLY may be a potential target for the treatment and prognosis of esophageal cancer. |
| format | Article |
| id | doaj-art-d716159e21e6498fa580372456602b6f |
| institution | Kabale University |
| issn | 1004-5511 |
| language | zho |
| publishDate | 2023-10-01 |
| publisher | Editorial Office of New Medicine |
| record_format | Article |
| series | Yixue xinzhi zazhi |
| spelling | doaj-art-d716159e21e6498fa580372456602b6f2024-12-25T10:09:55ZzhoEditorial Office of New MedicineYixue xinzhi zazhi1004-55112023-10-0133533434210.12173/j.issn.1004-5511.2022110026409Exploration of the relationship between ACLY and esophageal cancer based on bioinformatics and related experimentsMeng WANGYang-Yang ZHANGJing LIUObjective This study focuses on ATP citrate lyase (ACLY), takes esophageal cancer cells and online databases as the main research subjects, and further explores the expression of ACLY in esophageal cancer cells and its effects on cell growth and proliferation through bioinformatics analysis and cell biology experiments, in order to provide a new target for clinical diagnosis and treatment of esophageal cancer.Method The databases such as TCGA were used to analyze the expression of ACLY in esophageal cancer patients, as well as the expression of ACLY in esophageal cancer patients of different race groups and different clinical stages of esophageal cancer. At the same time, the effects of ACLY changes on the proliferation and cell cycle of esophageal cancer cells were detected by basic cell experiments such as CCK-8 method, EdU staining experiment, plate cloning experiment and flow cytometry.Results ACLY is highly expressed in various tumor tissues including esophageal cancer. The expression of ACLY is universal, independent of race and gender, and its expression is related to the staging of esophageal cancer and lymph node metastasis. Further experiments in cell biology confirmed that knocking down ACLY could inhibit the proliferation of esophageal cancer cells and block the cell cycle. Overexpression of ACLY could increase the proliferative capacity of esophageal cancer cells and promote cell cycle progression.Conclusion ACLY can affect the proliferation ability of esophageal cancer cells, and ACLY may be a potential target for the treatment and prognosis of esophageal cancer.https://yxxz.whuznhmedj.com/futureApi/storage/attach/2310/F7g35mN9wy0k9QizKTfyxem4viE7WklhRMp7V0Qx.pdfesophageal canceraclybioinformaticscell proliferation |
| spellingShingle | Meng WANG Yang-Yang ZHANG Jing LIU Exploration of the relationship between ACLY and esophageal cancer based on bioinformatics and related experiments Yixue xinzhi zazhi esophageal cancer acly bioinformatics cell proliferation |
| title | Exploration of the relationship between ACLY and esophageal cancer based on bioinformatics and related experiments |
| title_full | Exploration of the relationship between ACLY and esophageal cancer based on bioinformatics and related experiments |
| title_fullStr | Exploration of the relationship between ACLY and esophageal cancer based on bioinformatics and related experiments |
| title_full_unstemmed | Exploration of the relationship between ACLY and esophageal cancer based on bioinformatics and related experiments |
| title_short | Exploration of the relationship between ACLY and esophageal cancer based on bioinformatics and related experiments |
| title_sort | exploration of the relationship between acly and esophageal cancer based on bioinformatics and related experiments |
| topic | esophageal cancer acly bioinformatics cell proliferation |
| url | https://yxxz.whuznhmedj.com/futureApi/storage/attach/2310/F7g35mN9wy0k9QizKTfyxem4viE7WklhRMp7V0Qx.pdf |
| work_keys_str_mv | AT mengwang explorationoftherelationshipbetweenaclyandesophagealcancerbasedonbioinformaticsandrelatedexperiments AT yangyangzhang explorationoftherelationshipbetweenaclyandesophagealcancerbasedonbioinformaticsandrelatedexperiments AT jingliu explorationoftherelationshipbetweenaclyandesophagealcancerbasedonbioinformaticsandrelatedexperiments |