Condition optimization for electroporation transfection in horse skeletal muscle satellite cells
Satellite cells are an important cellular model for studying muscle growth and development and mammalian locomotion-related molecular mechanisms. In this study, we investigated the effects of voltage, pulse duration, and DNA dosage on horse skeletal muscle satellite cells’ electroporation transfecti...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Animal Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/10495398.2023.2280664 |
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| author | Tseweendolmaa Ulaangerel Minna Yi Undarmaa Budsuren Yingchao Shen Hong Ren Bold Demuul Dongyi Bai Dulguun Dorjgotov Gantulga Davaakhuu Tuyatsetseg Jambal Manglai Dugarjav Gerelchimeg Bou |
| author_facet | Tseweendolmaa Ulaangerel Minna Yi Undarmaa Budsuren Yingchao Shen Hong Ren Bold Demuul Dongyi Bai Dulguun Dorjgotov Gantulga Davaakhuu Tuyatsetseg Jambal Manglai Dugarjav Gerelchimeg Bou |
| author_sort | Tseweendolmaa Ulaangerel |
| collection | DOAJ |
| description | Satellite cells are an important cellular model for studying muscle growth and development and mammalian locomotion-related molecular mechanisms. In this study, we investigated the effects of voltage, pulse duration, and DNA dosage on horse skeletal muscle satellite cells’ electroporation transfection efficiency using the eukaryotic expression plasmid Td Tomato-C1 (5.5 kb) encoding the red fluorescent protein gene mainly based on fluorescence-positive cell rate and cell survival rate. By comparison of different voltages, pulse durations, and DNA doses, horse skeletal muscle satellite cells have nearly 80% transfection efficiency under the condition of voltage 120 V, DNA dosage 7 µg/ml, and pulse duration 30 ms. This optimized electroporation condition would facilitate the application of horse skeletal muscle satellite cells in genetic studies of muscle function and related diseases. |
| format | Article |
| id | doaj-art-d6f8bef933444d1784cea60fcf819c6e |
| institution | Kabale University |
| issn | 1049-5398 1532-2378 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Biotechnology |
| spelling | doaj-art-d6f8bef933444d1784cea60fcf819c6e2024-12-31T18:28:13ZengTaylor & Francis GroupAnimal Biotechnology1049-53981532-23782024-12-0135110.1080/10495398.2023.2280664Condition optimization for electroporation transfection in horse skeletal muscle satellite cellsTseweendolmaa Ulaangerel0Minna Yi1Undarmaa Budsuren2Yingchao Shen3Hong Ren4Bold Demuul5Dongyi Bai6Dulguun Dorjgotov7Gantulga Davaakhuu8Tuyatsetseg Jambal9Manglai Dugarjav10Gerelchimeg Bou11lnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, Chinalnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, Chinalnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, Chinalnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, Chinalnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, Chinalnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, Chinalnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, ChinaSchool of Industrial Technology, Mongolian University of Science and Technology, Ulaanbaatar, MongoliaInstitute of General and Experimental Biology, Mongolian Academy of Science, Ulaanbaatar, MongoliaSchool of Industrial Technology, Mongolian University of Science and Technology, Ulaanbaatar, Mongolialnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, Chinalnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, ChinaSatellite cells are an important cellular model for studying muscle growth and development and mammalian locomotion-related molecular mechanisms. In this study, we investigated the effects of voltage, pulse duration, and DNA dosage on horse skeletal muscle satellite cells’ electroporation transfection efficiency using the eukaryotic expression plasmid Td Tomato-C1 (5.5 kb) encoding the red fluorescent protein gene mainly based on fluorescence-positive cell rate and cell survival rate. By comparison of different voltages, pulse durations, and DNA doses, horse skeletal muscle satellite cells have nearly 80% transfection efficiency under the condition of voltage 120 V, DNA dosage 7 µg/ml, and pulse duration 30 ms. This optimized electroporation condition would facilitate the application of horse skeletal muscle satellite cells in genetic studies of muscle function and related diseases.https://www.tandfonline.com/doi/10.1080/10495398.2023.2280664Horsesatellite cellselectroporation transfectionparameter optimization |
| spellingShingle | Tseweendolmaa Ulaangerel Minna Yi Undarmaa Budsuren Yingchao Shen Hong Ren Bold Demuul Dongyi Bai Dulguun Dorjgotov Gantulga Davaakhuu Tuyatsetseg Jambal Manglai Dugarjav Gerelchimeg Bou Condition optimization for electroporation transfection in horse skeletal muscle satellite cells Animal Biotechnology Horse satellite cells electroporation transfection parameter optimization |
| title | Condition optimization for electroporation transfection in horse skeletal muscle satellite cells |
| title_full | Condition optimization for electroporation transfection in horse skeletal muscle satellite cells |
| title_fullStr | Condition optimization for electroporation transfection in horse skeletal muscle satellite cells |
| title_full_unstemmed | Condition optimization for electroporation transfection in horse skeletal muscle satellite cells |
| title_short | Condition optimization for electroporation transfection in horse skeletal muscle satellite cells |
| title_sort | condition optimization for electroporation transfection in horse skeletal muscle satellite cells |
| topic | Horse satellite cells electroporation transfection parameter optimization |
| url | https://www.tandfonline.com/doi/10.1080/10495398.2023.2280664 |
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