Biomechanical modeling of spatiotemporal bacteria-phage competition

Abstract Bacteriophages are viral predators of bacteria. Understanding the bacteria-phage competition is crucial for horizontal gene transfer and treatment of antibiotic-resistant bacterial infections. Here, we investigate the interaction dynamics between common rod-shaped bacteria such as Escherich...

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Bibliographic Details
Main Authors: Andrés Valdez, Hui Sun, Howard Howie Weiss, Igor Aranson
Format: Article
Language:English
Published: Nature Portfolio 2025-04-01
Series:Communications Physics
Online Access:https://doi.org/10.1038/s42005-025-02078-1
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Summary:Abstract Bacteriophages are viral predators of bacteria. Understanding the bacteria-phage competition is crucial for horizontal gene transfer and treatment of antibiotic-resistant bacterial infections. Here, we investigate the interaction dynamics between common rod-shaped bacteria such as Escherichia coli or Pseudomonas aeruginosa and lytic phages within 2D and 3D environments. Our computational study is based on established experimental observations of bacteria-phage interactions. A lytic phage effectively kills bacterial cells in a colony, leading to significant consequences for its morphology and expansion. Through computational modeling, we observe that phage interactions with bacteria produce phage-plaque regions characterized by cell death, reorganization, and altered colony growth dynamics. As phages predate, surviving cells tend to re-align toward the phage-affected region, forming a more ordered structure. This reordering effect not only reduces the radial spread of the colony but, in highly virulent scenarios, generates an inflow of cells toward the phage-plaque, effectively “devouring” portions of the colony. Our work highlights how phages control the self-organization of proliferating active matter and offers insights into targeted approaches for managing bacterial populations in biofilm-associated environments.
ISSN:2399-3650