Shotgun metagenomic analysis of the oral microbiome in gingivitis: a nested case-control study
Background Gingivitis, i.e. inflammation of the gums, is often induced by dental plaque. However, its exact link to the oral microbiota remains unclear.Methods In a case-control study involving 120 participants, comprising 60 cases and 60 controls (mean age (SD) 36.6 (7.6) years; 50% males), nested...
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Taylor & Francis Group
2024-12-01
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| Series: | Journal of Oral Microbiology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/20002297.2024.2330867 |
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| author | Muhammed Manzoor Jaakko Leskelä Milla Pietiäinen Nicolas Martinez-Majander Eija Könönen Teemu Niiranen Leo Lahti Juha Sinisalo Jukka Putaala Pirkko J. Pussinen Susanna Paju |
| author_facet | Muhammed Manzoor Jaakko Leskelä Milla Pietiäinen Nicolas Martinez-Majander Eija Könönen Teemu Niiranen Leo Lahti Juha Sinisalo Jukka Putaala Pirkko J. Pussinen Susanna Paju |
| author_sort | Muhammed Manzoor |
| collection | DOAJ |
| description | Background Gingivitis, i.e. inflammation of the gums, is often induced by dental plaque. However, its exact link to the oral microbiota remains unclear.Methods In a case-control study involving 120 participants, comprising 60 cases and 60 controls (mean age (SD) 36.6 (7.6) years; 50% males), nested within a prospective multicentre cohort study, we examined the oral microbiome composition of gingivitis patients and their controls using shotgun metagenomic sequencing of saliva samples. Participants underwent clinical and radiographic oral health examinations, including bleeding on probing (BOP), at six tooth sites. BOP ≥33% was considered ‘generalized gingivitis/initial periodontitis’(GG/IP), and BOP <33% as ‘healthy and localized gingivitis’(H/LG). Functional potential was inferred using HUMANn3.Results GG/IP exhibited an increase in the abundance of Actinomyces, Porphyromonas, Aggregatibacter, Corynebacterium, Olsenella, and Treponema, whereas H/LG exhibited an increased abundance of Candidatus Nanosynbacter. Nineteen bacterial species and four microbial functional profiles, including L-methionine, glycogen, and inosine-5’-phosphate biosynthesis, were associated with GG/IP. Constructing models with multiple markers resulted in a strong predictive value for GG/IP, with an area under the curve (ROC) of 0.907 (95% CI: 0.848-0.966).Conclusion We observed distinct differences in the oral microbiome between the GG/IP and H/LG groups, indicating similar yet unique microbial profiles and emphasizing their potential role in progression of periodontal diseases. |
| format | Article |
| id | doaj-art-d6ab7a99a1b840d1a5d5ad0508ae5d6c |
| institution | Kabale University |
| issn | 2000-2297 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj-art-d6ab7a99a1b840d1a5d5ad0508ae5d6c2024-11-26T06:52:11ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972024-12-0116110.1080/20002297.2024.2330867Shotgun metagenomic analysis of the oral microbiome in gingivitis: a nested case-control studyMuhammed Manzoor0Jaakko Leskelä1Milla Pietiäinen2Nicolas Martinez-Majander3Eija Könönen4Teemu Niiranen5Leo Lahti6Juha Sinisalo7Jukka Putaala8Pirkko J. Pussinen9Susanna Paju10Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, FinlandDepartment of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, FinlandDepartment of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, FinlandDepartment of Neurology, Helsinki University Hospital and University of Helsinki, Helsinki, FinlandInstitute of Dentistry, University of Turku, Turku, FinlandDepartment of Public Health and Welfare, Finnish Institute for Health and Welfare, Helsinki, FinlandDepartment of Computing, University of Turku, Turku, FinlandHeart and Lung Center, Helsinki University Central Hospital, and Helsinki University, Helsinki, FinlandDepartment of Neurology, Helsinki University Hospital and University of Helsinki, Helsinki, FinlandDepartment of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, FinlandDepartment of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, FinlandBackground Gingivitis, i.e. inflammation of the gums, is often induced by dental plaque. However, its exact link to the oral microbiota remains unclear.Methods In a case-control study involving 120 participants, comprising 60 cases and 60 controls (mean age (SD) 36.6 (7.6) years; 50% males), nested within a prospective multicentre cohort study, we examined the oral microbiome composition of gingivitis patients and their controls using shotgun metagenomic sequencing of saliva samples. Participants underwent clinical and radiographic oral health examinations, including bleeding on probing (BOP), at six tooth sites. BOP ≥33% was considered ‘generalized gingivitis/initial periodontitis’(GG/IP), and BOP <33% as ‘healthy and localized gingivitis’(H/LG). Functional potential was inferred using HUMANn3.Results GG/IP exhibited an increase in the abundance of Actinomyces, Porphyromonas, Aggregatibacter, Corynebacterium, Olsenella, and Treponema, whereas H/LG exhibited an increased abundance of Candidatus Nanosynbacter. Nineteen bacterial species and four microbial functional profiles, including L-methionine, glycogen, and inosine-5’-phosphate biosynthesis, were associated with GG/IP. Constructing models with multiple markers resulted in a strong predictive value for GG/IP, with an area under the curve (ROC) of 0.907 (95% CI: 0.848-0.966).Conclusion We observed distinct differences in the oral microbiome between the GG/IP and H/LG groups, indicating similar yet unique microbial profiles and emphasizing their potential role in progression of periodontal diseases.https://www.tandfonline.com/doi/10.1080/20002297.2024.2330867Metagenomicsoral microbiomeperiodontal diseasechronic periodontitissalivabiomarker |
| spellingShingle | Muhammed Manzoor Jaakko Leskelä Milla Pietiäinen Nicolas Martinez-Majander Eija Könönen Teemu Niiranen Leo Lahti Juha Sinisalo Jukka Putaala Pirkko J. Pussinen Susanna Paju Shotgun metagenomic analysis of the oral microbiome in gingivitis: a nested case-control study Journal of Oral Microbiology Metagenomics oral microbiome periodontal disease chronic periodontitis saliva biomarker |
| title | Shotgun metagenomic analysis of the oral microbiome in gingivitis: a nested case-control study |
| title_full | Shotgun metagenomic analysis of the oral microbiome in gingivitis: a nested case-control study |
| title_fullStr | Shotgun metagenomic analysis of the oral microbiome in gingivitis: a nested case-control study |
| title_full_unstemmed | Shotgun metagenomic analysis of the oral microbiome in gingivitis: a nested case-control study |
| title_short | Shotgun metagenomic analysis of the oral microbiome in gingivitis: a nested case-control study |
| title_sort | shotgun metagenomic analysis of the oral microbiome in gingivitis a nested case control study |
| topic | Metagenomics oral microbiome periodontal disease chronic periodontitis saliva biomarker |
| url | https://www.tandfonline.com/doi/10.1080/20002297.2024.2330867 |
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