Producción de etanol a partir de cebada no malteada hidrolizada con a y b amilasas comerciales
Ethanol production from hydrolyzed unmalted barley using commercial a- and b-amylase. Objective. To find the optimumconcentration of commercial a- and b-amylase for the obtainment of ethanol from unmalted barley. Materials and methods. Unmaltedbarley was hydrolyzed using various concentrations of co...
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| Language: | English |
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Pontificia Universidad Javeriana
2009-12-01
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| Series: | Universitas Scientiarum |
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| Online Access: | http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1387/849 |
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| author | Herberth Espitia-Rivera Carolina Espitia-Rocha Ivonne Gutierrez-Rojas |
| author_facet | Herberth Espitia-Rivera Carolina Espitia-Rocha Ivonne Gutierrez-Rojas |
| author_sort | Herberth Espitia-Rivera |
| collection | DOAJ |
| description | Ethanol production from hydrolyzed unmalted barley using commercial a- and b-amylase. Objective. To find the optimumconcentration of commercial a- and b-amylase for the obtainment of ethanol from unmalted barley. Materials and methods. Unmaltedbarley was hydrolyzed using various concentrations of commercial a- and b-amylase (Genencor International), following conditionsestablished by the manufacturer. The products of hydrolysis were used as substrates for the production of ethanol by Saccharomycescerevisiae. In addition, a reference assay was performed using malted barley following the conditions established by the distillery.Results. The percentage of starch hydrolysis was 89.4% when adding a-and b-amylase at a concentration of 1 g L-1. Moreover, thisconcentration of amylases yielded a maximum ethanol production (5.02 %) significantly higher than when malted barley was used (3.76%). Conclusions. It was demonstrated that ethanol can be obtained from starch of unmalted barley by adding commercial a- and b-amylase. However, optimization of the process is required due to the higher costs when compared to the traditional process with maltedbarley. |
| format | Article |
| id | doaj-art-d6a5d39da4544b51b887a1bafaa3c2bd |
| institution | Kabale University |
| issn | 0122-7483 2027-1352 |
| language | English |
| publishDate | 2009-12-01 |
| publisher | Pontificia Universidad Javeriana |
| record_format | Article |
| series | Universitas Scientiarum |
| spelling | doaj-art-d6a5d39da4544b51b887a1bafaa3c2bd2025-08-20T03:24:40ZengPontificia Universidad JaverianaUniversitas Scientiarum0122-74832027-13522009-12-01142-3164172Producción de etanol a partir de cebada no malteada hidrolizada con a y b amilasas comercialesHerberth Espitia-RiveraCarolina Espitia-RochaIvonne Gutierrez-RojasEthanol production from hydrolyzed unmalted barley using commercial a- and b-amylase. Objective. To find the optimumconcentration of commercial a- and b-amylase for the obtainment of ethanol from unmalted barley. Materials and methods. Unmaltedbarley was hydrolyzed using various concentrations of commercial a- and b-amylase (Genencor International), following conditionsestablished by the manufacturer. The products of hydrolysis were used as substrates for the production of ethanol by Saccharomycescerevisiae. In addition, a reference assay was performed using malted barley following the conditions established by the distillery.Results. The percentage of starch hydrolysis was 89.4% when adding a-and b-amylase at a concentration of 1 g L-1. Moreover, thisconcentration of amylases yielded a maximum ethanol production (5.02 %) significantly higher than when malted barley was used (3.76%). Conclusions. It was demonstrated that ethanol can be obtained from starch of unmalted barley by adding commercial a- and b-amylase. However, optimization of the process is required due to the higher costs when compared to the traditional process with maltedbarley.http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1387/849a-amylaseb-amylasefermentationSaccharomyces cerevisiaestarch hydrolysis. |
| spellingShingle | Herberth Espitia-Rivera Carolina Espitia-Rocha Ivonne Gutierrez-Rojas Producción de etanol a partir de cebada no malteada hidrolizada con a y b amilasas comerciales Universitas Scientiarum a-amylase b-amylase fermentation Saccharomyces cerevisiae starch hydrolysis. |
| title | Producción de etanol a partir de cebada no malteada hidrolizada con a y b amilasas comerciales |
| title_full | Producción de etanol a partir de cebada no malteada hidrolizada con a y b amilasas comerciales |
| title_fullStr | Producción de etanol a partir de cebada no malteada hidrolizada con a y b amilasas comerciales |
| title_full_unstemmed | Producción de etanol a partir de cebada no malteada hidrolizada con a y b amilasas comerciales |
| title_short | Producción de etanol a partir de cebada no malteada hidrolizada con a y b amilasas comerciales |
| title_sort | produccion de etanol a partir de cebada no malteada hidrolizada con a y b amilasas comerciales |
| topic | a-amylase b-amylase fermentation Saccharomyces cerevisiae starch hydrolysis. |
| url | http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1387/849 |
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