Producción de etanol a partir de cebada no malteada hidrolizada con a y b amilasas comerciales
Ethanol production from hydrolyzed unmalted barley using commercial a- and b-amylase. Objective. To find the optimumconcentration of commercial a- and b-amylase for the obtainment of ethanol from unmalted barley. Materials and methods. Unmaltedbarley was hydrolyzed using various concentrations of co...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Pontificia Universidad Javeriana
2009-12-01
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| Series: | Universitas Scientiarum |
| Subjects: | |
| Online Access: | http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1387/849 |
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| Summary: | Ethanol production from hydrolyzed unmalted barley using commercial a- and b-amylase. Objective. To find the optimumconcentration of commercial a- and b-amylase for the obtainment of ethanol from unmalted barley. Materials and methods. Unmaltedbarley was hydrolyzed using various concentrations of commercial a- and b-amylase (Genencor International), following conditionsestablished by the manufacturer. The products of hydrolysis were used as substrates for the production of ethanol by Saccharomycescerevisiae. In addition, a reference assay was performed using malted barley following the conditions established by the distillery.Results. The percentage of starch hydrolysis was 89.4% when adding a-and b-amylase at a concentration of 1 g L-1. Moreover, thisconcentration of amylases yielded a maximum ethanol production (5.02 %) significantly higher than when malted barley was used (3.76%). Conclusions. It was demonstrated that ethanol can be obtained from starch of unmalted barley by adding commercial a- and b-amylase. However, optimization of the process is required due to the higher costs when compared to the traditional process with maltedbarley. |
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| ISSN: | 0122-7483 2027-1352 |