High-intensity ethanol binge drinking accentuates bone damage in induced apical periodontitis in rats
This study aimed to evaluate the effects of excessive and episodic consumption of ethanol (EtOH, a high-intensity drinking manner) on induced apical periodontitis in rats. Thirty-two animals were divided into the following four groups: control, EtOH, apical periodontitis, and EtOH + apical periodont...
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Elsevier
2024-11-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024161944 |
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| author | José Mário Matos-Sousa Deiweson Souza-Monteiro Vinicius Ruan Neves dos Santos Maria Karolina Martins Ferreira Deborah Ribeiro Frazão Victória Santos Chemelo Leonardo de Oliveira Bittencourt João Daniel Mendonça de Moura Cristiane do Socorro Ferraz Maia Fabrício Mezzomo Collares Luanna de Melo Pereira Fernandes Rafael Rodrigues Lima |
| author_facet | José Mário Matos-Sousa Deiweson Souza-Monteiro Vinicius Ruan Neves dos Santos Maria Karolina Martins Ferreira Deborah Ribeiro Frazão Victória Santos Chemelo Leonardo de Oliveira Bittencourt João Daniel Mendonça de Moura Cristiane do Socorro Ferraz Maia Fabrício Mezzomo Collares Luanna de Melo Pereira Fernandes Rafael Rodrigues Lima |
| author_sort | José Mário Matos-Sousa |
| collection | DOAJ |
| description | This study aimed to evaluate the effects of excessive and episodic consumption of ethanol (EtOH, a high-intensity drinking manner) on induced apical periodontitis in rats. Thirty-two animals were divided into the following four groups: control, EtOH, apical periodontitis, and EtOH + apical periodontitis. Ethanol exposure (3 g/kg 20 % w/v EtOH) was performed by orogastric gavage for 3 consecutive days, followed by 4 days of withdrawal for 4 weeks. Lesions were induced by exposing the dental pulp of the lower first molar and by the absence of any treatment/curative for 28 days. Finally, the animals were euthanized, and mandibles were collected. The mandible was divided medially, with one hemimandible being used for micro-computed tomography analysis of the volume of the periapical lesion and bone quality parameters, such as bone volume and trabecular bone assessments; the other hemimandible was used for histological analysis, with a descriptive histopathological analysis of the tissue and the pattern of bone loss presented, as well as an assessment of the collagen content present. The data were subjected to statistical analysis (one-way analysis of variance with Tukey's post-hoc test). Our results showed that the EtOH + apical periodontitis group had a larger volume of periapical lesions than animals that were not exposed to ethanol. Additionally, bone quality parameters showed a reduction in bone volume and thickening of the trabeculae, associated with increased tissue destruction and reduced collagen content in the remnant region of the alveolar bone. These results suggest that exposure to EtOH in a pattern of excessive alcohol consumption is an aggravating factor in apical periodontitis and, consequently, in its progression, the quality and quantity of the alveolar bone remaining in the region of the periapical lesion are the modulating aspects. |
| format | Article |
| id | doaj-art-d653a15e2f064255ab7d25393f5b62d4 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Elsevier |
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| series | Heliyon |
| spelling | doaj-art-d653a15e2f064255ab7d25393f5b62d42024-11-30T07:11:57ZengElsevierHeliyon2405-84402024-11-011022e40163High-intensity ethanol binge drinking accentuates bone damage in induced apical periodontitis in ratsJosé Mário Matos-Sousa0Deiweson Souza-Monteiro1Vinicius Ruan Neves dos Santos2Maria Karolina Martins Ferreira3Deborah Ribeiro Frazão4Victória Santos Chemelo5Leonardo de Oliveira Bittencourt6João Daniel Mendonça de Moura7Cristiane do Socorro Ferraz Maia8Fabrício Mezzomo Collares9Luanna de Melo Pereira Fernandes10Rafael Rodrigues Lima11Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém, Pará, BrazilLaboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém, Pará, BrazilLaboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém, Pará, BrazilLaboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém, Pará, BrazilLaboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém, Pará, BrazilLaboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém, Pará, BrazilLaboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém, Pará, BrazilLaboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém, Pará, BrazilLaboratory of Inflammation and Behavioral Pharmacology, Institute of Health Sciences, Federal University of Pará, Belém, Pará, BrazilLaboratory of Dental Materials, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, BrazilLaboratory of Neuropharmacology and Behavior, Center for Biological |Health Sciences, State University of Pará, Belém, Pará, BrazilLaboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém, Pará, Brazil; Corresponding author. Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Augusto Corrêa Street, Guamá, 66075-110, Belém, PA, Brazil.This study aimed to evaluate the effects of excessive and episodic consumption of ethanol (EtOH, a high-intensity drinking manner) on induced apical periodontitis in rats. Thirty-two animals were divided into the following four groups: control, EtOH, apical periodontitis, and EtOH + apical periodontitis. Ethanol exposure (3 g/kg 20 % w/v EtOH) was performed by orogastric gavage for 3 consecutive days, followed by 4 days of withdrawal for 4 weeks. Lesions were induced by exposing the dental pulp of the lower first molar and by the absence of any treatment/curative for 28 days. Finally, the animals were euthanized, and mandibles were collected. The mandible was divided medially, with one hemimandible being used for micro-computed tomography analysis of the volume of the periapical lesion and bone quality parameters, such as bone volume and trabecular bone assessments; the other hemimandible was used for histological analysis, with a descriptive histopathological analysis of the tissue and the pattern of bone loss presented, as well as an assessment of the collagen content present. The data were subjected to statistical analysis (one-way analysis of variance with Tukey's post-hoc test). Our results showed that the EtOH + apical periodontitis group had a larger volume of periapical lesions than animals that were not exposed to ethanol. Additionally, bone quality parameters showed a reduction in bone volume and thickening of the trabeculae, associated with increased tissue destruction and reduced collagen content in the remnant region of the alveolar bone. These results suggest that exposure to EtOH in a pattern of excessive alcohol consumption is an aggravating factor in apical periodontitis and, consequently, in its progression, the quality and quantity of the alveolar bone remaining in the region of the periapical lesion are the modulating aspects.http://www.sciencedirect.com/science/article/pii/S2405844024161944Apical periodontitisBinge drinkingHigh-intensity drinkingAlveolar bone loss |
| spellingShingle | José Mário Matos-Sousa Deiweson Souza-Monteiro Vinicius Ruan Neves dos Santos Maria Karolina Martins Ferreira Deborah Ribeiro Frazão Victória Santos Chemelo Leonardo de Oliveira Bittencourt João Daniel Mendonça de Moura Cristiane do Socorro Ferraz Maia Fabrício Mezzomo Collares Luanna de Melo Pereira Fernandes Rafael Rodrigues Lima High-intensity ethanol binge drinking accentuates bone damage in induced apical periodontitis in rats Heliyon Apical periodontitis Binge drinking High-intensity drinking Alveolar bone loss |
| title | High-intensity ethanol binge drinking accentuates bone damage in induced apical periodontitis in rats |
| title_full | High-intensity ethanol binge drinking accentuates bone damage in induced apical periodontitis in rats |
| title_fullStr | High-intensity ethanol binge drinking accentuates bone damage in induced apical periodontitis in rats |
| title_full_unstemmed | High-intensity ethanol binge drinking accentuates bone damage in induced apical periodontitis in rats |
| title_short | High-intensity ethanol binge drinking accentuates bone damage in induced apical periodontitis in rats |
| title_sort | high intensity ethanol binge drinking accentuates bone damage in induced apical periodontitis in rats |
| topic | Apical periodontitis Binge drinking High-intensity drinking Alveolar bone loss |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024161944 |
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