Examination and evaluation of PCR-based candidate RGA markers in rice
Based on 402 co-dominant rice candidate RGA markers developed by bioinformatics methods, 40 pairs of primers were randomly selected to test the polymorphisms in rice two subspecies, japonica and indica. The results showed that 87.5% (35 markers) of the total number of markers obtained the clear band...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2007-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2007.01.0051 |
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| Summary: | Based on 402 co-dominant rice candidate RGA markers developed by bioinformatics methods, 40 pairs of primers were randomly selected to test the polymorphisms in rice two subspecies, japonica and indica. The results showed that 87.5% (35 markers) of the total number of markers obtained the clear bands, which 31 markers were co-dominant and 4 markers were dominant. In addition, 31 primer pairs were used to analyze 24 different japonica and indica varieties, and the result showed the markers have good commonality and subspecies-specificity. The averaged Polymorphism Information Content (PIC) of RGA markers was 0.46, indicated that the RGA markers have good polymorphism. |
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| ISSN: | 1008-9209 2097-5155 |