用液相芯片技术定量测定人血清CEA、AFP和HBsAg
【目的】采用液相芯片技术同时定量测定人血清中癌胚抗原(CEA)、甲胎蛋白(AFP)和乙肝表面抗原(HBsAg),并对该方法进行评价。【方法】制备抗体交联微球及生物素标记抗体,用双抗体夹心法对临床血清标本进行测定。【结果】同时检测CEA、AFP和HBsAg时的线性范围分别为0.032～200ng/mL、0.022～55U/mL、0.26～1000ng/mL,最低检测限分别为8.90pg/mL、0.013U/mL、0.24ng/mL,批内变异系数(CV)<9%,批间CV<14%。检测CEA、AFP、HBsAg的灵敏度分别为96.4%、95.8%、92%,特异度分别为95.6%、94.2...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Sun Yat-sen University
2007-01-01
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| Series: | Zhongshan Daxue xuebao. Yixue kexue ban |
| Subjects: | |
| Online Access: | http://xuebaoyx.sysu.edu.cn/zh/article/43582442/ |
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