Think twice: how single-cell suspensions from solid tissues for single-cell transcriptomics are well prepared
Abstract. Due to its technological advantages, single-cell sequencing has become an increasingly utilized tool to unravel the heterogeneity and complexity of individuals. The preparation of a high-quality single-cell suspension from solid tissues is a critical step prior to single-cell RNA sequencin...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Health - Lippincott Williams Wilkins
2025-01-01
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| Series: | Infectious Diseases & Immunity |
| Online Access: | http://journals.lww.com/10.1097/ID9.0000000000000136 |
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| author | Meng-Meng Qu Yan-Mei Jiao Haijuan Wang |
| author_facet | Meng-Meng Qu Yan-Mei Jiao Haijuan Wang |
| author_sort | Meng-Meng Qu |
| collection | DOAJ |
| description | Abstract. Due to its technological advantages, single-cell sequencing has become an increasingly utilized tool to unravel the heterogeneity and complexity of individuals. The preparation of a high-quality single-cell suspension from solid tissues is a critical step prior to single-cell RNA sequencing. Based on a brief overview of the steps involved in preparing cell suspensions from solid tissues for single-cell RNA sequencing, we present a detailed analysis of the pivotal steps in the preparation process, including enzymatic digestion, mechanical dissociation, and cell viability evaluation. The clarification of these experimental details will greatly help us obtain high-quality single-cell sequencing data from solid tissues. |
| format | Article |
| id | doaj-art-d56c2977473c43d5842d47a3f6291c9a |
| institution | Kabale University |
| issn | 2096-9511 2693-8839 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wolters Kluwer Health - Lippincott Williams Wilkins |
| record_format | Article |
| series | Infectious Diseases & Immunity |
| spelling | doaj-art-d56c2977473c43d5842d47a3f6291c9a2025-01-14T07:30:25ZengWolters Kluwer Health - Lippincott Williams WilkinsInfectious Diseases & Immunity2096-95112693-88392025-01-0151727410.1097/ID9.0000000000000136202501000-00009Think twice: how single-cell suspensions from solid tissues for single-cell transcriptomics are well preparedMeng-Meng Qu0Yan-Mei Jiao1Haijuan WangSenior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, National Clinical Research Center for Infectious Diseases, Beijing 100039, ChinaSenior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, National Clinical Research Center for Infectious Diseases, Beijing 100039, ChinaAbstract. Due to its technological advantages, single-cell sequencing has become an increasingly utilized tool to unravel the heterogeneity and complexity of individuals. The preparation of a high-quality single-cell suspension from solid tissues is a critical step prior to single-cell RNA sequencing. Based on a brief overview of the steps involved in preparing cell suspensions from solid tissues for single-cell RNA sequencing, we present a detailed analysis of the pivotal steps in the preparation process, including enzymatic digestion, mechanical dissociation, and cell viability evaluation. The clarification of these experimental details will greatly help us obtain high-quality single-cell sequencing data from solid tissues.http://journals.lww.com/10.1097/ID9.0000000000000136 |
| spellingShingle | Meng-Meng Qu Yan-Mei Jiao Haijuan Wang Think twice: how single-cell suspensions from solid tissues for single-cell transcriptomics are well prepared Infectious Diseases & Immunity |
| title | Think twice: how single-cell suspensions from solid tissues for single-cell transcriptomics are well prepared |
| title_full | Think twice: how single-cell suspensions from solid tissues for single-cell transcriptomics are well prepared |
| title_fullStr | Think twice: how single-cell suspensions from solid tissues for single-cell transcriptomics are well prepared |
| title_full_unstemmed | Think twice: how single-cell suspensions from solid tissues for single-cell transcriptomics are well prepared |
| title_short | Think twice: how single-cell suspensions from solid tissues for single-cell transcriptomics are well prepared |
| title_sort | think twice how single cell suspensions from solid tissues for single cell transcriptomics are well prepared |
| url | http://journals.lww.com/10.1097/ID9.0000000000000136 |
| work_keys_str_mv | AT mengmengqu thinktwicehowsinglecellsuspensionsfromsolidtissuesforsinglecelltranscriptomicsarewellprepared AT yanmeijiao thinktwicehowsinglecellsuspensionsfromsolidtissuesforsinglecelltranscriptomicsarewellprepared AT haijuanwang thinktwicehowsinglecellsuspensionsfromsolidtissuesforsinglecelltranscriptomicsarewellprepared |