Utility of CB-NAAT in cutaneous tuberculosis: A prospective observational study
Problem considered: Conventional diagnostic techniques for diagnosing Cutaneous tuberculosis (CTB) have variable sensitivity, specificity and are time-consuming. CB-NAAT is based on real-time PCR and can be helpful in rapid detection of mycobacterial antigens along with the detection of rifampicin r...
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Elsevier
2025-03-01
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| Series: | Clinical Epidemiology and Global Health |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213398425000053 |
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| author | Parul Verma Srishti Tripathi Swastika Suvirya Prakriti Shukla Amita Jain Atin Singhai Urmila Singh Nishant Verma |
| author_facet | Parul Verma Srishti Tripathi Swastika Suvirya Prakriti Shukla Amita Jain Atin Singhai Urmila Singh Nishant Verma |
| author_sort | Parul Verma |
| collection | DOAJ |
| description | Problem considered: Conventional diagnostic techniques for diagnosing Cutaneous tuberculosis (CTB) have variable sensitivity, specificity and are time-consuming. CB-NAAT is based on real-time PCR and can be helpful in rapid detection of mycobacterial antigens along with the detection of rifampicin resistance. Our study aims at detecting the efficacy of CB-NAAT in the diagnosis of CTB as compared to conventional diagnostic techniques like histopathology and culture. Methods: Skin biopsies were taken from the representative lesions in 41 patients of CTB. One sample was sent for histopathology whereas the other sample was sent for CB-NAAT, liquid culture -DST (drug sensitivity testing) and smear microscopy. Results: Out of 41 patients with cutaneous TB, CB-NAAT was positive in 6 (14.63 %), culture was positive in 8 (19.5 %) and histopathology was compatible in 26 (63.4 %) patients. CB-NAAT was positive in 3 out of 8 culture positive cases and 4 out of 26 histopathology compatible cases. The sensitivity and specificity of gene Xpert MTB/RIF in detecting TB when compared to culture were 37.5 % and 91 % respectively and Positive- and Negative-predictive values were 50 % and 85.7 % respectively. Xpert MTB/RIF could identify an additional 3 cases, where all other microbiological investigations were negative. Out of 6 cases that were CB-NAAT positive, 3 showed Rifampicin resistance. Conclusions: The CB-NAAT test has variable diagnostic accuracy in the diagnosis of CTB which can vary with the clinical specimen. It can prove as a promising tool for early detection of cutaneous TB along with drug-resistant TB especially in the high endemic zones. |
| format | Article |
| id | doaj-art-d55a3fb3df644c488964f13a0590a49c |
| institution | Kabale University |
| issn | 2213-3984 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Clinical Epidemiology and Global Health |
| spelling | doaj-art-d55a3fb3df644c488964f13a0590a49c2025-01-11T06:41:21ZengElsevierClinical Epidemiology and Global Health2213-39842025-03-0132101916Utility of CB-NAAT in cutaneous tuberculosis: A prospective observational studyParul Verma0Srishti Tripathi1Swastika Suvirya2Prakriti Shukla3Amita Jain4Atin Singhai5Urmila Singh6Nishant Verma7Dermatology Venereology and Leprosy, King George's Medical University, Lucknow, U.P, India; Corresponding author.Dermatology Venereology and Leprosy, Netaji Subhash Chandra Bose Medical College, Jabalpur, M.P, IndiaDermatology Venereology and Leprosy, King George's Medical University, Lucknow, U.P, IndiaDepartment of Dermatology, Maharshi Devraha Baba Autonomous State Medical College, Deoria, U.P, IndiaMicrobiology, King George's Medical University, Lucknow, U.P, IndiaPathology, King George's Medical University, Lucknow, U.P, IndiaDepartment of Microbiology, King George's Medical University, Lucknow, U.P, IndiaDepartment of Paediatrics, King George's Medical University, Lucknow, U.P, IndiaProblem considered: Conventional diagnostic techniques for diagnosing Cutaneous tuberculosis (CTB) have variable sensitivity, specificity and are time-consuming. CB-NAAT is based on real-time PCR and can be helpful in rapid detection of mycobacterial antigens along with the detection of rifampicin resistance. Our study aims at detecting the efficacy of CB-NAAT in the diagnosis of CTB as compared to conventional diagnostic techniques like histopathology and culture. Methods: Skin biopsies were taken from the representative lesions in 41 patients of CTB. One sample was sent for histopathology whereas the other sample was sent for CB-NAAT, liquid culture -DST (drug sensitivity testing) and smear microscopy. Results: Out of 41 patients with cutaneous TB, CB-NAAT was positive in 6 (14.63 %), culture was positive in 8 (19.5 %) and histopathology was compatible in 26 (63.4 %) patients. CB-NAAT was positive in 3 out of 8 culture positive cases and 4 out of 26 histopathology compatible cases. The sensitivity and specificity of gene Xpert MTB/RIF in detecting TB when compared to culture were 37.5 % and 91 % respectively and Positive- and Negative-predictive values were 50 % and 85.7 % respectively. Xpert MTB/RIF could identify an additional 3 cases, where all other microbiological investigations were negative. Out of 6 cases that were CB-NAAT positive, 3 showed Rifampicin resistance. Conclusions: The CB-NAAT test has variable diagnostic accuracy in the diagnosis of CTB which can vary with the clinical specimen. It can prove as a promising tool for early detection of cutaneous TB along with drug-resistant TB especially in the high endemic zones.http://www.sciencedirect.com/science/article/pii/S2213398425000053Cutaneous tuberculosisCB-NAATPCR in skin tuberculosis |
| spellingShingle | Parul Verma Srishti Tripathi Swastika Suvirya Prakriti Shukla Amita Jain Atin Singhai Urmila Singh Nishant Verma Utility of CB-NAAT in cutaneous tuberculosis: A prospective observational study Clinical Epidemiology and Global Health Cutaneous tuberculosis CB-NAAT PCR in skin tuberculosis |
| title | Utility of CB-NAAT in cutaneous tuberculosis: A prospective observational study |
| title_full | Utility of CB-NAAT in cutaneous tuberculosis: A prospective observational study |
| title_fullStr | Utility of CB-NAAT in cutaneous tuberculosis: A prospective observational study |
| title_full_unstemmed | Utility of CB-NAAT in cutaneous tuberculosis: A prospective observational study |
| title_short | Utility of CB-NAAT in cutaneous tuberculosis: A prospective observational study |
| title_sort | utility of cb naat in cutaneous tuberculosis a prospective observational study |
| topic | Cutaneous tuberculosis CB-NAAT PCR in skin tuberculosis |
| url | http://www.sciencedirect.com/science/article/pii/S2213398425000053 |
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