Physiological and molecular mechanisms of leaf response to high-temperature stress in high-temperature-resistant soybean varieties
Abstract Background With increasing global limate warm, high temperature (HT) is one of limiting factors for soybean yield and quality. Exploring HT resistance-related functional genes and their corresponding molecular mechanisms is of great value. In our previous report, compared with HD14 (HT sens...
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2024-11-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-024-10932-9 |
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| author | Jiajia Li Xianguan Zhi Haoran Chen Linying Chen Yun Lu Wei Liao Zhuo Tian Meiyan Wu Yajing Shan Heng Wang Long Yan Bingqiang Liu Xiaobo Wang |
| author_facet | Jiajia Li Xianguan Zhi Haoran Chen Linying Chen Yun Lu Wei Liao Zhuo Tian Meiyan Wu Yajing Shan Heng Wang Long Yan Bingqiang Liu Xiaobo Wang |
| author_sort | Jiajia Li |
| collection | DOAJ |
| description | Abstract Background With increasing global limate warm, high temperature (HT) is one of limiting factors for soybean yield and quality. Exploring HT resistance-related functional genes and their corresponding molecular mechanisms is of great value. In our previous report, compared with HD14 (HT sensitive), JD21 is an HT-resistant variety, and further analysis of the transcriptome and proteome has revealed the HT tolerance mechanism of JD21 anthers. We found that compared with those of HD14 (28.72%), the leaves of JD21 also exhibited HT resistance, and the degree of leaf wilting in JD21 plants after HT stress treatment was 11.02%; however, the regulatory mechanism of the response of JD21 to HT stress is still unclear. Results In this study, comparative transcriptome analysis of JD21 and HD14 soybean leaves after HT stress and field control plants was performed by RNA-seq analysis. The results showed that the number of upregulated differentially expressed genes (DEGs) in JD21 and HD14 was greater than the number of downregulated DEGs after HT stress, and the number of up- or down-regulated DEGs in JD21 was higher than those of HD14. Bioinformatics analysis revealed that many DEGs were involved in various molecular functions and metabolic pathways. QRT‒PCR analysis verified that the gene expression pattern results determined via RNA–seq was reliable. In addition, through analysis of gene expression level and conserved domain, 18 key candidate genes related to the response of soybean leaves to HT stress were screened. Conclusions This study systematically revealed the regulation mechanism of soybean leaves molecular transcription level by RNA-seq, and several key candidate DEGs (transcription factor, HSPs, HSFs, GmCYP78A6, etc.) involved in the response to HT stress were identified based on the bioinformatics analysis. The results provided a theoretical basis for studying the response mechanism of soybean leaves to HT stress. |
| format | Article |
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| institution | Kabale University |
| issn | 1471-2164 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj-art-d555b3cab13d447a867f67f3de63582d2024-12-01T12:11:14ZengBMCBMC Genomics1471-21642024-11-0125111210.1186/s12864-024-10932-9Physiological and molecular mechanisms of leaf response to high-temperature stress in high-temperature-resistant soybean varietiesJiajia Li0Xianguan Zhi1Haoran Chen2Linying Chen3Yun Lu4Wei Liao5Zhuo Tian6Meiyan Wu7Yajing Shan8Heng Wang9Long Yan10Bingqiang Liu11Xiaobo Wang12School of Agronomy, Anhui Agricultural UniversitySchool of Agronomy, Anhui Agricultural UniversitySchool of Agronomy, Anhui Agricultural UniversitySchool of Agronomy, Anhui Agricultural UniversitySchool of Agronomy, Anhui Agricultural UniversitySchool of Agronomy, Anhui Agricultural UniversitySchool of Agronomy, Anhui Agricultural UniversitySchool of Agronomy, Anhui Agricultural UniversitySchool of Agronomy, Anhui Agricultural UniversitySchool of Agronomy, Anhui Agricultural UniversityNational Soybean Improvement Center Shijiazhuang Sub-Center, Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry SciencesNational Soybean Improvement Center Shijiazhuang Sub-Center, Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry SciencesSchool of Agronomy, Anhui Agricultural UniversityAbstract Background With increasing global limate warm, high temperature (HT) is one of limiting factors for soybean yield and quality. Exploring HT resistance-related functional genes and their corresponding molecular mechanisms is of great value. In our previous report, compared with HD14 (HT sensitive), JD21 is an HT-resistant variety, and further analysis of the transcriptome and proteome has revealed the HT tolerance mechanism of JD21 anthers. We found that compared with those of HD14 (28.72%), the leaves of JD21 also exhibited HT resistance, and the degree of leaf wilting in JD21 plants after HT stress treatment was 11.02%; however, the regulatory mechanism of the response of JD21 to HT stress is still unclear. Results In this study, comparative transcriptome analysis of JD21 and HD14 soybean leaves after HT stress and field control plants was performed by RNA-seq analysis. The results showed that the number of upregulated differentially expressed genes (DEGs) in JD21 and HD14 was greater than the number of downregulated DEGs after HT stress, and the number of up- or down-regulated DEGs in JD21 was higher than those of HD14. Bioinformatics analysis revealed that many DEGs were involved in various molecular functions and metabolic pathways. QRT‒PCR analysis verified that the gene expression pattern results determined via RNA–seq was reliable. In addition, through analysis of gene expression level and conserved domain, 18 key candidate genes related to the response of soybean leaves to HT stress were screened. Conclusions This study systematically revealed the regulation mechanism of soybean leaves molecular transcription level by RNA-seq, and several key candidate DEGs (transcription factor, HSPs, HSFs, GmCYP78A6, etc.) involved in the response to HT stress were identified based on the bioinformatics analysis. The results provided a theoretical basis for studying the response mechanism of soybean leaves to HT stress.https://doi.org/10.1186/s12864-024-10932-9SoybeanLeavesHigh-temperature stressRNA-seqMolecular mechanismConserved domain analysis |
| spellingShingle | Jiajia Li Xianguan Zhi Haoran Chen Linying Chen Yun Lu Wei Liao Zhuo Tian Meiyan Wu Yajing Shan Heng Wang Long Yan Bingqiang Liu Xiaobo Wang Physiological and molecular mechanisms of leaf response to high-temperature stress in high-temperature-resistant soybean varieties BMC Genomics Soybean Leaves High-temperature stress RNA-seq Molecular mechanism Conserved domain analysis |
| title | Physiological and molecular mechanisms of leaf response to high-temperature stress in high-temperature-resistant soybean varieties |
| title_full | Physiological and molecular mechanisms of leaf response to high-temperature stress in high-temperature-resistant soybean varieties |
| title_fullStr | Physiological and molecular mechanisms of leaf response to high-temperature stress in high-temperature-resistant soybean varieties |
| title_full_unstemmed | Physiological and molecular mechanisms of leaf response to high-temperature stress in high-temperature-resistant soybean varieties |
| title_short | Physiological and molecular mechanisms of leaf response to high-temperature stress in high-temperature-resistant soybean varieties |
| title_sort | physiological and molecular mechanisms of leaf response to high temperature stress in high temperature resistant soybean varieties |
| topic | Soybean Leaves High-temperature stress RNA-seq Molecular mechanism Conserved domain analysis |
| url | https://doi.org/10.1186/s12864-024-10932-9 |
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