Adjuvant postbiotic administration improves dental caries prognosis by restoring the oral microbiota
Conventional filling therapy fails to fundamentally reduce oral cariogenic bacteria. Thus, oral microbiota follow-up intervention after filling would be necessary for improving dental caries prognosis. We recruited 9 caries-free individuals, and 89 dental caries subjects (5 dropouts). Eighty-nine pa...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2024-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250217 |
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| author | Qing Liu Teng Ma Cuijiao Feng Yalin Li Hao Jin Xuan Shi Lai-Yu Kwok Yan Shi Tingtao Chen Heping Zhang |
| author_facet | Qing Liu Teng Ma Cuijiao Feng Yalin Li Hao Jin Xuan Shi Lai-Yu Kwok Yan Shi Tingtao Chen Heping Zhang |
| author_sort | Qing Liu |
| collection | DOAJ |
| description | Conventional filling therapy fails to fundamentally reduce oral cariogenic bacteria. Thus, oral microbiota follow-up intervention after filling would be necessary for improving dental caries prognosis. We recruited 9 caries-free individuals, and 89 dental caries subjects (5 dropouts). Eighty-nine patients were randomized into three groups: caries (n=8; no treatment), control (n=40; filling), and postbiotics (n=41; filling and 14-day Probio-Eco® intervention). Salivary samples were collected at 0 day (after filling) and 14 days. Our results showed that the diversity of dental caries oral microbiota was significantly increased compared with healthy subjects, and filling could restore a healthier oral microbiota partially and temporarily. Thepostbiotics intervention keeps a low alpha-diversity. Co-occurrence network analysis showed that a more stable oral microbiota structure after postbiotics intervention. Taxonomic and functional annotation of the microbiota revealed that postbiotics co-treatment significantly: increased the relative abundance of Pseudomonas and P. reactans, decreased the relative abundance of Prevotella shahii, and enriched the energy metabolism-related pathways. BugBase-predicted phenotypes inferred to an oral microbiota with decreased potential pathogenic bacteria and increased oxidative stress-tolerant bacteria after postbiotics intervention. Collectively, it suggested that postbiotics co-treatment could be a promising strategy that restores the oral microecological balance for dental caries. |
| format | Article |
| id | doaj-art-d534fbfa27fd43a7bc5cf73d27e6ed0d |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-d534fbfa27fd43a7bc5cf73d27e6ed0d2025-01-10T06:56:56ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-09-011352690270210.26599/FSHW.2022.9250217Adjuvant postbiotic administration improves dental caries prognosis by restoring the oral microbiotaQing Liu0Teng Ma1Cuijiao Feng2Yalin Li3Hao Jin4Xuan Shi5Lai-Yu Kwok6Yan Shi7Tingtao Chen8Heping Zhang9Affiliated Stomatological Hospital of Nanchang University, Nanchang 330000, ChinaInner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot 010000, ChinaInner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot 010000, ChinaInner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot 010000, ChinaInner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot 010000, ChinaInner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot 010000, ChinaInner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot 010000, ChinaAffiliated Stomatological Hospital of Nanchang University, Nanchang 330000, ChinaNational Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Nanchang University, Nanchang 330000, ChinaInner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot 010000, ChinaConventional filling therapy fails to fundamentally reduce oral cariogenic bacteria. Thus, oral microbiota follow-up intervention after filling would be necessary for improving dental caries prognosis. We recruited 9 caries-free individuals, and 89 dental caries subjects (5 dropouts). Eighty-nine patients were randomized into three groups: caries (n=8; no treatment), control (n=40; filling), and postbiotics (n=41; filling and 14-day Probio-Eco® intervention). Salivary samples were collected at 0 day (after filling) and 14 days. Our results showed that the diversity of dental caries oral microbiota was significantly increased compared with healthy subjects, and filling could restore a healthier oral microbiota partially and temporarily. Thepostbiotics intervention keeps a low alpha-diversity. Co-occurrence network analysis showed that a more stable oral microbiota structure after postbiotics intervention. Taxonomic and functional annotation of the microbiota revealed that postbiotics co-treatment significantly: increased the relative abundance of Pseudomonas and P. reactans, decreased the relative abundance of Prevotella shahii, and enriched the energy metabolism-related pathways. BugBase-predicted phenotypes inferred to an oral microbiota with decreased potential pathogenic bacteria and increased oxidative stress-tolerant bacteria after postbiotics intervention. Collectively, it suggested that postbiotics co-treatment could be a promising strategy that restores the oral microecological balance for dental caries.https://www.sciopen.com/article/10.26599/FSHW.2022.9250217postbioticsoral microbiotasalivary microbiotadental cariespacific biosciences (pacbio) single molecule real-time (smrt) sequencingdental filling |
| spellingShingle | Qing Liu Teng Ma Cuijiao Feng Yalin Li Hao Jin Xuan Shi Lai-Yu Kwok Yan Shi Tingtao Chen Heping Zhang Adjuvant postbiotic administration improves dental caries prognosis by restoring the oral microbiota Food Science and Human Wellness postbiotics oral microbiota salivary microbiota dental caries pacific biosciences (pacbio) single molecule real-time (smrt) sequencing dental filling |
| title | Adjuvant postbiotic administration improves dental caries prognosis by restoring the oral microbiota |
| title_full | Adjuvant postbiotic administration improves dental caries prognosis by restoring the oral microbiota |
| title_fullStr | Adjuvant postbiotic administration improves dental caries prognosis by restoring the oral microbiota |
| title_full_unstemmed | Adjuvant postbiotic administration improves dental caries prognosis by restoring the oral microbiota |
| title_short | Adjuvant postbiotic administration improves dental caries prognosis by restoring the oral microbiota |
| title_sort | adjuvant postbiotic administration improves dental caries prognosis by restoring the oral microbiota |
| topic | postbiotics oral microbiota salivary microbiota dental caries pacific biosciences (pacbio) single molecule real-time (smrt) sequencing dental filling |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250217 |
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