Drug resistance to delamanid was detected in susceptible tuberculosis patient in Russia
AIM: To detect mutations in M. tuberculosis (MTB) associated with delamanid drug resistance in patients with tuberculosis (TB) in Russia. BACKGROUND: Delamanid is important in treatment of multi- and extensively drug-resistant TB. Reports on delamanid drug resistance are increasing making it necessa...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716524003643 |
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| author | Platon Eliseev Irina Vasilyeva Anatoly Vinokurov Evgeny Kuznetsov Kirill Baibekov Anastasia Kazulina Anastasia Samoilova |
| author_facet | Platon Eliseev Irina Vasilyeva Anatoly Vinokurov Evgeny Kuznetsov Kirill Baibekov Anastasia Kazulina Anastasia Samoilova |
| author_sort | Platon Eliseev |
| collection | DOAJ |
| description | AIM: To detect mutations in M. tuberculosis (MTB) associated with delamanid drug resistance in patients with tuberculosis (TB) in Russia. BACKGROUND: Delamanid is important in treatment of multi- and extensively drug-resistant TB. Reports on delamanid drug resistance are increasing making it necessary to perform drug susceptibility testing (DST). METHODS: In 2023 full-genome next-generation sequencing (NGS) and DST of 364 MTB isolates from TB patients in different regions of Russia were performed. RESULTS: Drug resistance to delamanid was detected in five MTB isolates. In all 5 isolates mutations in the gene ddn Trp88* (tGg > tAg) were detected. Resistance was confirmed by Bactec MGIT DST with critical concentration 1.0 μg/mL. Interestingly, one isolate with drug resistance to delamanid was susceptible to other drugs (Table). Four other isolates had different combinations of drug resistance to rifampicin (rpoB Ser450Leu), isoniazid (katG Ser315Thr), ethambutol (embB Asp354Ala), streptomycin (rpsL Lys43Arg), pyrazinamide (pncA Asp63Ala), ethionamide (ethA 1614del and ethA 1615del), amikacin and capreomycin (rrs 1401A>G), moxifloxacin (gyrA Asp94Gly and gyrA Asp94Ala), para‐aminosalicylic acid (folC Glu40Gly) and cycloserine (ald 130dupG). Drug resistance to bedaquiline and linezolid was not detected in those isolates. CONCLUSION: Drug resistance to delamanid was detected in patients that were not previously exposed to delamanid, including one patient without any additional drug resistance suggesting that all patients should be tested for drug resistance before treatment initiation regardless of their treatment history. |
| format | Article |
| id | doaj-art-d50f42b0251c49e4906e8a6c00e960f5 |
| institution | Kabale University |
| issn | 2213-7165 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj-art-d50f42b0251c49e4906e8a6c00e960f52024-12-27T04:08:44ZengElsevierJournal of Global Antimicrobial Resistance2213-71652024-12-013959Drug resistance to delamanid was detected in susceptible tuberculosis patient in RussiaPlaton Eliseev0Irina Vasilyeva1Anatoly Vinokurov2Evgeny Kuznetsov3Kirill Baibekov4Anastasia Kazulina5Anastasia Samoilova6The National Medical Research Center of Phthisiopulmonology and Infectious Diseases under the Ministry of Health of the Russian Federation, Moscow, RussiaThe National Medical Research Center of Phthisiopulmonology and Infectious Diseases under the Ministry of Health of the Russian Federation, Moscow, RussiaThe National Medical Research Center of Phthisiopulmonology and Infectious Diseases under the Ministry of Health of the Russian Federation, Moscow, RussiaThe National Medical Research Center of Phthisiopulmonology and Infectious Diseases under the Ministry of Health of the Russian Federation, Moscow, RussiaThe National Medical Research Center of Phthisiopulmonology and Infectious Diseases under the Ministry of Health of the Russian Federation, Moscow, RussiaThe National Medical Research Center of Phthisiopulmonology and Infectious Diseases under the Ministry of Health of the Russian Federation, Moscow, RussiaThe National Medical Research Center of Phthisiopulmonology and Infectious Diseases under the Ministry of Health of the Russian Federation, Moscow, RussiaAIM: To detect mutations in M. tuberculosis (MTB) associated with delamanid drug resistance in patients with tuberculosis (TB) in Russia. BACKGROUND: Delamanid is important in treatment of multi- and extensively drug-resistant TB. Reports on delamanid drug resistance are increasing making it necessary to perform drug susceptibility testing (DST). METHODS: In 2023 full-genome next-generation sequencing (NGS) and DST of 364 MTB isolates from TB patients in different regions of Russia were performed. RESULTS: Drug resistance to delamanid was detected in five MTB isolates. In all 5 isolates mutations in the gene ddn Trp88* (tGg > tAg) were detected. Resistance was confirmed by Bactec MGIT DST with critical concentration 1.0 μg/mL. Interestingly, one isolate with drug resistance to delamanid was susceptible to other drugs (Table). Four other isolates had different combinations of drug resistance to rifampicin (rpoB Ser450Leu), isoniazid (katG Ser315Thr), ethambutol (embB Asp354Ala), streptomycin (rpsL Lys43Arg), pyrazinamide (pncA Asp63Ala), ethionamide (ethA 1614del and ethA 1615del), amikacin and capreomycin (rrs 1401A>G), moxifloxacin (gyrA Asp94Gly and gyrA Asp94Ala), para‐aminosalicylic acid (folC Glu40Gly) and cycloserine (ald 130dupG). Drug resistance to bedaquiline and linezolid was not detected in those isolates. CONCLUSION: Drug resistance to delamanid was detected in patients that were not previously exposed to delamanid, including one patient without any additional drug resistance suggesting that all patients should be tested for drug resistance before treatment initiation regardless of their treatment history.http://www.sciencedirect.com/science/article/pii/S2213716524003643tuberculosisdelamaniddrug resistancesequencing |
| spellingShingle | Platon Eliseev Irina Vasilyeva Anatoly Vinokurov Evgeny Kuznetsov Kirill Baibekov Anastasia Kazulina Anastasia Samoilova Drug resistance to delamanid was detected in susceptible tuberculosis patient in Russia Journal of Global Antimicrobial Resistance tuberculosis delamanid drug resistance sequencing |
| title | Drug resistance to delamanid was detected in susceptible tuberculosis patient in Russia |
| title_full | Drug resistance to delamanid was detected in susceptible tuberculosis patient in Russia |
| title_fullStr | Drug resistance to delamanid was detected in susceptible tuberculosis patient in Russia |
| title_full_unstemmed | Drug resistance to delamanid was detected in susceptible tuberculosis patient in Russia |
| title_short | Drug resistance to delamanid was detected in susceptible tuberculosis patient in Russia |
| title_sort | drug resistance to delamanid was detected in susceptible tuberculosis patient in russia |
| topic | tuberculosis delamanid drug resistance sequencing |
| url | http://www.sciencedirect.com/science/article/pii/S2213716524003643 |
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