Structural elucidation of mulberry leaf oligosaccharide and its selective promotion of gut microbiota to alleviate type 2 diabetes mellitus
Two oligosaccharide fractions (MLO 2-1 and 2-2) were purified from enzymatic hydrolysate of mulberry leaf polysaccharide. The results of simulated digestion showed that MLO 2-2 was a digestible oligosaccharide, which could be degraded by human digestive juice; while MLO 2-1 possessed the non-digesti...
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Tsinghua University Press
2024-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250180 |
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| author | Tenggen Hu Yuanshan Yu Jijun Wu Yujuan Xu Gengsheng Xiao Kejing An Erna Li Sentai Liao Yuxiao Zou |
| author_facet | Tenggen Hu Yuanshan Yu Jijun Wu Yujuan Xu Gengsheng Xiao Kejing An Erna Li Sentai Liao Yuxiao Zou |
| author_sort | Tenggen Hu |
| collection | DOAJ |
| description | Two oligosaccharide fractions (MLO 2-1 and 2-2) were purified from enzymatic hydrolysate of mulberry leaf polysaccharide. The results of simulated digestion showed that MLO 2-2 was a digestible oligosaccharide, which could be degraded by human digestive juice; while MLO 2-1 possessed the non-digestible property in the upper gastrointestinal tract and performed the function by regulating the gut microbiota. Hence, MLO 2-1 was selected for the further analysis. The structure of MLO 2-1 was elucidated as follow: α-T-Glcp-(1→3)-β-Glcp-(1→5)-α-Araf-(1→5)-α-Araf-1→5)-α-Araf-(1→3)-α-(2-OAc)-Glcp-1. The in vitro fecal fermentation results showed that MLO 2-1 could modulate the composition of gut microbiota. Meanwhile, MLO 2-1 was effectively metabolized by fecal bacteria to produce lactate and short chain fatty acids, especially acetate and butyrate. The specific metabolic pathways of MLO 2-1 by gut microbiota were further illuminated. Gut microbiota analysis revealed that MLO 2-1 selectively promoted the growth of Ligilactobacillus murinus, a commensal bacterium presented a reduced level in T2DM mice. Animal experiments indicated that MLO 2-1 and L. murinus exhibited hypoglycemic activities. These results demonstrated that MLO 2-1 might alleviate T2DM by selectively accelerating the proliferation of L. murinus. |
| format | Article |
| id | doaj-art-d4cc3f28876840f39981493cd38ab3db |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-d4cc3f28876840f39981493cd38ab3db2025-01-10T06:56:48ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-07-011342161217310.26599/FSHW.2022.9250180Structural elucidation of mulberry leaf oligosaccharide and its selective promotion of gut microbiota to alleviate type 2 diabetes mellitusTenggen Hu0Yuanshan Yu1Jijun Wu2Yujuan Xu3Gengsheng Xiao4Kejing An5Erna Li6Sentai Liao7Yuxiao Zou8Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, ChinaSericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, ChinaSericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, ChinaSericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, ChinaZhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaSericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, ChinaSericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, ChinaSericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, ChinaSericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, ChinaTwo oligosaccharide fractions (MLO 2-1 and 2-2) were purified from enzymatic hydrolysate of mulberry leaf polysaccharide. The results of simulated digestion showed that MLO 2-2 was a digestible oligosaccharide, which could be degraded by human digestive juice; while MLO 2-1 possessed the non-digestible property in the upper gastrointestinal tract and performed the function by regulating the gut microbiota. Hence, MLO 2-1 was selected for the further analysis. The structure of MLO 2-1 was elucidated as follow: α-T-Glcp-(1→3)-β-Glcp-(1→5)-α-Araf-(1→5)-α-Araf-1→5)-α-Araf-(1→3)-α-(2-OAc)-Glcp-1. The in vitro fecal fermentation results showed that MLO 2-1 could modulate the composition of gut microbiota. Meanwhile, MLO 2-1 was effectively metabolized by fecal bacteria to produce lactate and short chain fatty acids, especially acetate and butyrate. The specific metabolic pathways of MLO 2-1 by gut microbiota were further illuminated. Gut microbiota analysis revealed that MLO 2-1 selectively promoted the growth of Ligilactobacillus murinus, a commensal bacterium presented a reduced level in T2DM mice. Animal experiments indicated that MLO 2-1 and L. murinus exhibited hypoglycemic activities. These results demonstrated that MLO 2-1 might alleviate T2DM by selectively accelerating the proliferation of L. murinus.https://www.sciopen.com/article/10.26599/FSHW.2022.9250180mulberry leaf oligosaccharidecharacterizationgut microbiotalactobacillus murinushypoglycemic activity |
| spellingShingle | Tenggen Hu Yuanshan Yu Jijun Wu Yujuan Xu Gengsheng Xiao Kejing An Erna Li Sentai Liao Yuxiao Zou Structural elucidation of mulberry leaf oligosaccharide and its selective promotion of gut microbiota to alleviate type 2 diabetes mellitus Food Science and Human Wellness mulberry leaf oligosaccharide characterization gut microbiota lactobacillus murinus hypoglycemic activity |
| title | Structural elucidation of mulberry leaf oligosaccharide and its selective promotion of gut microbiota to alleviate type 2 diabetes mellitus |
| title_full | Structural elucidation of mulberry leaf oligosaccharide and its selective promotion of gut microbiota to alleviate type 2 diabetes mellitus |
| title_fullStr | Structural elucidation of mulberry leaf oligosaccharide and its selective promotion of gut microbiota to alleviate type 2 diabetes mellitus |
| title_full_unstemmed | Structural elucidation of mulberry leaf oligosaccharide and its selective promotion of gut microbiota to alleviate type 2 diabetes mellitus |
| title_short | Structural elucidation of mulberry leaf oligosaccharide and its selective promotion of gut microbiota to alleviate type 2 diabetes mellitus |
| title_sort | structural elucidation of mulberry leaf oligosaccharide and its selective promotion of gut microbiota to alleviate type 2 diabetes mellitus |
| topic | mulberry leaf oligosaccharide characterization gut microbiota lactobacillus murinus hypoglycemic activity |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250180 |
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