Impacts of Nano-Composite of Copper and Carbon on Intestinal Luminal Micro-Ecosystem and Mucosal Homeostasis of Yellow-Feather Broilers
The present study was undertaken to evaluate the impacts of nano-composites of copper and carbon (NCCC) on the intestinal luminal micro-ecosystem and mucosal homeostasis of yellow-feather broilers. A total of two-hundred and forty 1-day-old male yellow-feather broilers were randomly allocated into f...
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2024-11-01
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| author | Xianglin Wang Chunlong Xiao Shuqing Wu Qingjie Lin Shiying Lin Jing Liu Dingcheng Ye Changkang Wang Pingting Guo |
| author_facet | Xianglin Wang Chunlong Xiao Shuqing Wu Qingjie Lin Shiying Lin Jing Liu Dingcheng Ye Changkang Wang Pingting Guo |
| author_sort | Xianglin Wang |
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| description | The present study was undertaken to evaluate the impacts of nano-composites of copper and carbon (NCCC) on the intestinal luminal micro-ecosystem and mucosal homeostasis of yellow-feather broilers. A total of two-hundred and forty 1-day-old male yellow-feather broilers were randomly allocated into four groups, each with five replications of twelve birds. The control (CON) group received a corn-soybean basal diet, while the N50, N100, and N200 groups were supplemented with 50, 100, and 200 mg/kg of NCCC in basal diets, respectively. The trial duration was 63 days. The findings demonstrated that there were slight impacts of NCCC addition on the intestinal luminal micro-ecosystem of broilers, with the fecal moisture content in the N100 group being slightly higher on Day 3 in the starter phase (<i>p</i> < 0.05). The cecal microbiota structure also did not obviously change (<i>p</i> > 0.05), in spite of the fall in the relative abundance of the <i>Ruminococcus torques group</i> in the N50 group and <i>norank Clostridia UCG-014</i> in N200 group (<i>p</i> < 0.05). But for intestinal mucosal homeostasis, NCCC played a crucial part in jejunal morphology, tight junction, immunologic status, and antioxidant capacity. There was linear growth in villus height and a quadratic increase in villus height, crypt depth and their ratio with the increase in NCCC dosage (<i>p</i> < 0.05), and 100 mg/kg NCCC supplementation could intensify the expression of <i>CLDN</i>-3 genes (<i>p</i> < 0.05). In addition, IL-4 and IL-10 linearly increased after NCCC treatment (<i>p</i> < 0.05), along with some irregular changes in sIgA (<i>p</i> < 0.05). In addition, higher jejunal mucosal total antioxidant capacities in N50 and N200 groups were also observed (<i>p</i> < 0.05). Overall, NCCC treatment optimized the intestinal mucosa function of broilers in terms of physical barrier and immune and antioxidant capacities, but exerted subtle influence in the luminal environment of yellow-feather broilers. More precisely, dietary supplementation with 50 mg/kg NCCC is recommended for intestinal homeostasis of broilers. |
| format | Article |
| id | doaj-art-d421885888fc4ef79a47e0fcf7e77f85 |
| institution | Kabale University |
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| language | English |
| publishDate | 2024-11-01 |
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| spelling | doaj-art-d421885888fc4ef79a47e0fcf7e77f852024-11-26T18:14:39ZengMDPI AGMicroorganisms2076-26072024-11-011211224710.3390/microorganisms12112247Impacts of Nano-Composite of Copper and Carbon on Intestinal Luminal Micro-Ecosystem and Mucosal Homeostasis of Yellow-Feather BroilersXianglin Wang0Chunlong Xiao1Shuqing Wu2Qingjie Lin3Shiying Lin4Jing Liu5Dingcheng Ye6Changkang Wang7Pingting Guo8College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaCollege of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaThe present study was undertaken to evaluate the impacts of nano-composites of copper and carbon (NCCC) on the intestinal luminal micro-ecosystem and mucosal homeostasis of yellow-feather broilers. A total of two-hundred and forty 1-day-old male yellow-feather broilers were randomly allocated into four groups, each with five replications of twelve birds. The control (CON) group received a corn-soybean basal diet, while the N50, N100, and N200 groups were supplemented with 50, 100, and 200 mg/kg of NCCC in basal diets, respectively. The trial duration was 63 days. The findings demonstrated that there were slight impacts of NCCC addition on the intestinal luminal micro-ecosystem of broilers, with the fecal moisture content in the N100 group being slightly higher on Day 3 in the starter phase (<i>p</i> < 0.05). The cecal microbiota structure also did not obviously change (<i>p</i> > 0.05), in spite of the fall in the relative abundance of the <i>Ruminococcus torques group</i> in the N50 group and <i>norank Clostridia UCG-014</i> in N200 group (<i>p</i> < 0.05). But for intestinal mucosal homeostasis, NCCC played a crucial part in jejunal morphology, tight junction, immunologic status, and antioxidant capacity. There was linear growth in villus height and a quadratic increase in villus height, crypt depth and their ratio with the increase in NCCC dosage (<i>p</i> < 0.05), and 100 mg/kg NCCC supplementation could intensify the expression of <i>CLDN</i>-3 genes (<i>p</i> < 0.05). In addition, IL-4 and IL-10 linearly increased after NCCC treatment (<i>p</i> < 0.05), along with some irregular changes in sIgA (<i>p</i> < 0.05). In addition, higher jejunal mucosal total antioxidant capacities in N50 and N200 groups were also observed (<i>p</i> < 0.05). Overall, NCCC treatment optimized the intestinal mucosa function of broilers in terms of physical barrier and immune and antioxidant capacities, but exerted subtle influence in the luminal environment of yellow-feather broilers. More precisely, dietary supplementation with 50 mg/kg NCCC is recommended for intestinal homeostasis of broilers.https://www.mdpi.com/2076-2607/12/11/2247Cu-C nano-compositeschickenmechanical barrierimmunitybacterial community |
| spellingShingle | Xianglin Wang Chunlong Xiao Shuqing Wu Qingjie Lin Shiying Lin Jing Liu Dingcheng Ye Changkang Wang Pingting Guo Impacts of Nano-Composite of Copper and Carbon on Intestinal Luminal Micro-Ecosystem and Mucosal Homeostasis of Yellow-Feather Broilers Microorganisms Cu-C nano-composites chicken mechanical barrier immunity bacterial community |
| title | Impacts of Nano-Composite of Copper and Carbon on Intestinal Luminal Micro-Ecosystem and Mucosal Homeostasis of Yellow-Feather Broilers |
| title_full | Impacts of Nano-Composite of Copper and Carbon on Intestinal Luminal Micro-Ecosystem and Mucosal Homeostasis of Yellow-Feather Broilers |
| title_fullStr | Impacts of Nano-Composite of Copper and Carbon on Intestinal Luminal Micro-Ecosystem and Mucosal Homeostasis of Yellow-Feather Broilers |
| title_full_unstemmed | Impacts of Nano-Composite of Copper and Carbon on Intestinal Luminal Micro-Ecosystem and Mucosal Homeostasis of Yellow-Feather Broilers |
| title_short | Impacts of Nano-Composite of Copper and Carbon on Intestinal Luminal Micro-Ecosystem and Mucosal Homeostasis of Yellow-Feather Broilers |
| title_sort | impacts of nano composite of copper and carbon on intestinal luminal micro ecosystem and mucosal homeostasis of yellow feather broilers |
| topic | Cu-C nano-composites chicken mechanical barrier immunity bacterial community |
| url | https://www.mdpi.com/2076-2607/12/11/2247 |
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