Microbial communities in the phyllosphere and endosphere of Norway spruce under attack by Heterobasidion
Heterobasidion annosum species complex has been regarded as the most destructive disease agent of conifer trees in boreal forests. Tree microbiome can regulate the plant–pathogen interactions by influencing both host resistance and pathogen virulence. Such information would help to improve the futur...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1489900/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841555195762311168 |
|---|---|
| author | Wen-jing Meng Zi-lan Wen Risto Kasanen Hui Sun Hui Sun Fred O. Asiegbu |
| author_facet | Wen-jing Meng Zi-lan Wen Risto Kasanen Hui Sun Hui Sun Fred O. Asiegbu |
| author_sort | Wen-jing Meng |
| collection | DOAJ |
| description | Heterobasidion annosum species complex has been regarded as the most destructive disease agent of conifer trees in boreal forests. Tree microbiome can regulate the plant–pathogen interactions by influencing both host resistance and pathogen virulence. Such information would help to improve the future health of forests and explore strategies to enhance ecosystem stability. In this study, using next-generation sequencing technology, we investigated the microbial community in different tree regions (needles, upper stem, and lower stem) of Norway spruce with and without wood decay symptoms. The primary purpose was to uncover signature characteristic microbiome harbored by asymptomatic trees compared to diseased trees. Additionally, the study was to explore the inter-kingdom and intra-kingdom interactions in microbiome (bacteria and fungi) of symptomatic versus asymptomatic trees. The results showed that in upper stem, species richness (Chao1) of fungi and bacteria were both higher in asymptomatic trees than symptomatic trees (P < 0.05). Compared to symptomatic trees, asymptomatic trees harbored a higher abundance of Actinobacteriota, bacterial genera of Methylocella, Conexibacter, Jatrophihabitans, and fungal genera of Mollisia. Fungal communities from the same anatomic region differed between the symptomatic and asymptomatic trees. Bacterial communities from the two stem regions were also distinct between the symptomatic and asymptomatic trees. The symptomatic trees possessed a less stable microbial network with more positive correlations compared to the asymptomatic trees. In the lower stem, at intra-kingdom level, the distribution of correlation numbers was more even in the bacterial network compared to the fungal network. In conclusion, the Heterobasidion attack decreased the microbial community species richness and shifted the community structure and functional structure to varying degrees. The microbial network was enlarged and became more unstable at both inter-kingdom and intra-kingdom level due to the Heterobasidion infection. |
| format | Article |
| id | doaj-art-d3f5e0239687447bb04a4088a7713620 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-d3f5e0239687447bb04a4088a77136202025-01-08T06:11:48ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011510.3389/fmicb.2024.14899001489900Microbial communities in the phyllosphere and endosphere of Norway spruce under attack by HeterobasidionWen-jing Meng0Zi-lan Wen1Risto Kasanen2Hui Sun3Hui Sun4Fred O. Asiegbu5Department of Forest Sciences, Faculty of Agriculture and Forestry, University of Helsinki, Helsinki, FinlandDepartment of Forest Sciences, Faculty of Agriculture and Forestry, University of Helsinki, Helsinki, FinlandDepartment of Forest Sciences, Faculty of Agriculture and Forestry, University of Helsinki, Helsinki, FinlandDepartment of Forest Sciences, Faculty of Agriculture and Forestry, University of Helsinki, Helsinki, FinlandCollaborative Innovation Center of Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing, ChinaDepartment of Forest Sciences, Faculty of Agriculture and Forestry, University of Helsinki, Helsinki, FinlandHeterobasidion annosum species complex has been regarded as the most destructive disease agent of conifer trees in boreal forests. Tree microbiome can regulate the plant–pathogen interactions by influencing both host resistance and pathogen virulence. Such information would help to improve the future health of forests and explore strategies to enhance ecosystem stability. In this study, using next-generation sequencing technology, we investigated the microbial community in different tree regions (needles, upper stem, and lower stem) of Norway spruce with and without wood decay symptoms. The primary purpose was to uncover signature characteristic microbiome harbored by asymptomatic trees compared to diseased trees. Additionally, the study was to explore the inter-kingdom and intra-kingdom interactions in microbiome (bacteria and fungi) of symptomatic versus asymptomatic trees. The results showed that in upper stem, species richness (Chao1) of fungi and bacteria were both higher in asymptomatic trees than symptomatic trees (P < 0.05). Compared to symptomatic trees, asymptomatic trees harbored a higher abundance of Actinobacteriota, bacterial genera of Methylocella, Conexibacter, Jatrophihabitans, and fungal genera of Mollisia. Fungal communities from the same anatomic region differed between the symptomatic and asymptomatic trees. Bacterial communities from the two stem regions were also distinct between the symptomatic and asymptomatic trees. The symptomatic trees possessed a less stable microbial network with more positive correlations compared to the asymptomatic trees. In the lower stem, at intra-kingdom level, the distribution of correlation numbers was more even in the bacterial network compared to the fungal network. In conclusion, the Heterobasidion attack decreased the microbial community species richness and shifted the community structure and functional structure to varying degrees. The microbial network was enlarged and became more unstable at both inter-kingdom and intra-kingdom level due to the Heterobasidion infection.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1489900/fullmicrobial community structureHeterobasidionnetwork analysismicrobial interactionmicrobial community diversity |
| spellingShingle | Wen-jing Meng Zi-lan Wen Risto Kasanen Hui Sun Hui Sun Fred O. Asiegbu Microbial communities in the phyllosphere and endosphere of Norway spruce under attack by Heterobasidion Frontiers in Microbiology microbial community structure Heterobasidion network analysis microbial interaction microbial community diversity |
| title | Microbial communities in the phyllosphere and endosphere of Norway spruce under attack by Heterobasidion |
| title_full | Microbial communities in the phyllosphere and endosphere of Norway spruce under attack by Heterobasidion |
| title_fullStr | Microbial communities in the phyllosphere and endosphere of Norway spruce under attack by Heterobasidion |
| title_full_unstemmed | Microbial communities in the phyllosphere and endosphere of Norway spruce under attack by Heterobasidion |
| title_short | Microbial communities in the phyllosphere and endosphere of Norway spruce under attack by Heterobasidion |
| title_sort | microbial communities in the phyllosphere and endosphere of norway spruce under attack by heterobasidion |
| topic | microbial community structure Heterobasidion network analysis microbial interaction microbial community diversity |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1489900/full |
| work_keys_str_mv | AT wenjingmeng microbialcommunitiesinthephyllosphereandendosphereofnorwayspruceunderattackbyheterobasidion AT zilanwen microbialcommunitiesinthephyllosphereandendosphereofnorwayspruceunderattackbyheterobasidion AT ristokasanen microbialcommunitiesinthephyllosphereandendosphereofnorwayspruceunderattackbyheterobasidion AT huisun microbialcommunitiesinthephyllosphereandendosphereofnorwayspruceunderattackbyheterobasidion AT huisun microbialcommunitiesinthephyllosphereandendosphereofnorwayspruceunderattackbyheterobasidion AT fredoasiegbu microbialcommunitiesinthephyllosphereandendosphereofnorwayspruceunderattackbyheterobasidion |