Optimisation study on the flavonoid extraction process from Abrus precatorius leaves and the comparison of total flavonoid content by HPLC and UV
Abrus precatorius is a leguminous plant with high medicinal value. Its leaves are rich in flavonoids. There only are limited reports on the extraction process and quality assessment of total flavonoids in Abrus precatorius leaves (APL). This study presents the extraction process of total flavonoids...
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KeAi Communications Co., Ltd.
2023-10-01
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| Series: | Journal of Holistic Integrative Pharmacy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2707368823000869 |
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| author | Xujiang Yuan Cuimin He Xianmei Xue Zhike Liang Yanfen Chen |
| author_facet | Xujiang Yuan Cuimin He Xianmei Xue Zhike Liang Yanfen Chen |
| author_sort | Xujiang Yuan |
| collection | DOAJ |
| description | Abrus precatorius is a leguminous plant with high medicinal value. Its leaves are rich in flavonoids. There only are limited reports on the extraction process and quality assessment of total flavonoids in Abrus precatorius leaves (APL). This study presents the extraction process of total flavonoids in APL and its optimisation by response surface methodology (RSM). High-performance liquid chromatography (HPLC) and UV methods were used to determine the total flavonoid content and their differences were compared. The feasible extraction parameters of total flavonoids from APL were found to be 50% ethanol, a liquid-solid ratio of 50:1, an extraction time of 60 min and two extraction cycles. The number of extraction cycles was the main influencing factor. The total flavonoid content as determined by this optimised process was 69.0 mg/g by HPLC and 41.7 mg/g by UV. There was a significant difference between the total flavonoid content determined by the UV method and HPLC. A comparative analysis of 29 samples in RSM and 14 batches of APL showed that the total flavonoid content as determined by UV was only about half of that determined by HPLC. The factors that influence this difference and potential solutions to this challenge were discussed. The results of this study could lay a foundation for the quality assessment, standardisation, development and utilisation of flavonoids in APL. |
| format | Article |
| id | doaj-art-d3f4ee6b09904c8fae23bb5488ec7e8e |
| institution | Kabale University |
| issn | 2707-3688 |
| language | English |
| publishDate | 2023-10-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Holistic Integrative Pharmacy |
| spelling | doaj-art-d3f4ee6b09904c8fae23bb5488ec7e8e2024-11-12T05:21:41ZengKeAi Communications Co., Ltd.Journal of Holistic Integrative Pharmacy2707-36882023-10-0142119126Optimisation study on the flavonoid extraction process from Abrus precatorius leaves and the comparison of total flavonoid content by HPLC and UVXujiang Yuan0Cuimin He1Xianmei Xue2Zhike Liang3Yanfen Chen4Center for New Drug Research and Development/Guangdong Provincial Key Laboratory of New Drug Dosage Form / Key Laboratory of Modern Chinese Medicine, Department of Education of Guangdong Province/Traditional Chinese Medicine Preparation Laboratory, National Administration of Traditional Chinese Medicine (LevelⅢ), Guangdong Pharmaceutical University, Guangzhou, 510006, China; Corresponding author.Center for New Drug Research and Development/Guangdong Provincial Key Laboratory of New Drug Dosage Form / Key Laboratory of Modern Chinese Medicine, Department of Education of Guangdong Province/Traditional Chinese Medicine Preparation Laboratory, National Administration of Traditional Chinese Medicine (LevelⅢ), Guangdong Pharmaceutical University, Guangzhou, 510006, ChinaCenter for New Drug Research and Development/Guangdong Provincial Key Laboratory of New Drug Dosage Form / Key Laboratory of Modern Chinese Medicine, Department of Education of Guangdong Province/Traditional Chinese Medicine Preparation Laboratory, National Administration of Traditional Chinese Medicine (LevelⅢ), Guangdong Pharmaceutical University, Guangzhou, 510006, ChinaCenter for New Drug Research and Development/Guangdong Provincial Key Laboratory of New Drug Dosage Form / Key Laboratory of Modern Chinese Medicine, Department of Education of Guangdong Province/Traditional Chinese Medicine Preparation Laboratory, National Administration of Traditional Chinese Medicine (LevelⅢ), Guangdong Pharmaceutical University, Guangzhou, 510006, ChinaSchool of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, 510006, ChinaAbrus precatorius is a leguminous plant with high medicinal value. Its leaves are rich in flavonoids. There only are limited reports on the extraction process and quality assessment of total flavonoids in Abrus precatorius leaves (APL). This study presents the extraction process of total flavonoids in APL and its optimisation by response surface methodology (RSM). High-performance liquid chromatography (HPLC) and UV methods were used to determine the total flavonoid content and their differences were compared. The feasible extraction parameters of total flavonoids from APL were found to be 50% ethanol, a liquid-solid ratio of 50:1, an extraction time of 60 min and two extraction cycles. The number of extraction cycles was the main influencing factor. The total flavonoid content as determined by this optimised process was 69.0 mg/g by HPLC and 41.7 mg/g by UV. There was a significant difference between the total flavonoid content determined by the UV method and HPLC. A comparative analysis of 29 samples in RSM and 14 batches of APL showed that the total flavonoid content as determined by UV was only about half of that determined by HPLC. The factors that influence this difference and potential solutions to this challenge were discussed. The results of this study could lay a foundation for the quality assessment, standardisation, development and utilisation of flavonoids in APL.http://www.sciencedirect.com/science/article/pii/S2707368823000869Abrus precatorius leavesUVHigh-performance liquid chromatographyResponse surface methodologyTotal flavonoids |
| spellingShingle | Xujiang Yuan Cuimin He Xianmei Xue Zhike Liang Yanfen Chen Optimisation study on the flavonoid extraction process from Abrus precatorius leaves and the comparison of total flavonoid content by HPLC and UV Journal of Holistic Integrative Pharmacy Abrus precatorius leaves UV High-performance liquid chromatography Response surface methodology Total flavonoids |
| title | Optimisation study on the flavonoid extraction process from Abrus precatorius leaves and the comparison of total flavonoid content by HPLC and UV |
| title_full | Optimisation study on the flavonoid extraction process from Abrus precatorius leaves and the comparison of total flavonoid content by HPLC and UV |
| title_fullStr | Optimisation study on the flavonoid extraction process from Abrus precatorius leaves and the comparison of total flavonoid content by HPLC and UV |
| title_full_unstemmed | Optimisation study on the flavonoid extraction process from Abrus precatorius leaves and the comparison of total flavonoid content by HPLC and UV |
| title_short | Optimisation study on the flavonoid extraction process from Abrus precatorius leaves and the comparison of total flavonoid content by HPLC and UV |
| title_sort | optimisation study on the flavonoid extraction process from abrus precatorius leaves and the comparison of total flavonoid content by hplc and uv |
| topic | Abrus precatorius leaves UV High-performance liquid chromatography Response surface methodology Total flavonoids |
| url | http://www.sciencedirect.com/science/article/pii/S2707368823000869 |
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