Genome-Wide Analysis of C2H2 Zinc Finger Gene Family and Exploration of Genes Related to Prickle Development in <i>Zanthoxylum armatum</i>
<i>Zanthoxylum armatum</i> (<i>Z. armatum</i>) is a significant economic tree species known for its medicinal and edible properties. However, the presence of prickles on <i>Z. armatum</i> poses a considerable challenge to the advancement of its industry. Numerous...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
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| Series: | Horticulturae |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-7524/10/12/1371 |
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| Summary: | <i>Zanthoxylum armatum</i> (<i>Z. armatum</i>) is a significant economic tree species known for its medicinal and edible properties. However, the presence of prickles on <i>Z. armatum</i> poses a considerable challenge to the advancement of its industry. Numerous studies have indicated that the C2H2 zinc finger protein (C2H2-ZFPs) families are crucial in the development of plant trichomes or prickles. This study identified 78 <i>ZaC2H2</i> genes from the <i>Z. armatum</i> genome, categorizing them into three groups and analyzing their protein physicochemical properties, chromosomal locations, conserved domains, and gene structures. The evolutionary analysis indicates that the amplification of <i>ZaC2H2</i> genes primarily results from whole-genome duplication or segmental duplication, and these genes have undergone strong purifying selection pressure throughout their evolutionary history. The analysis of <i>cis</i>-acting elements revealed that they contain various hormone response elements, such as ABRE, AuxRR, the CGTCA motif, GARE motifs, and TCA elements, which are responsive to ABA, IAA, MeJA, GA, and SA signals. RT-qPCR was employed to assess the expression levels of the candidate genes <i>ZaC2H2-45</i>, <i>ZaC2H2-46</i>, <i>ZaC2H2-49,</i> and <i>ZaC2H2-55</i> under the treatment of five hormones. The results indicated that the expression levels of the <i>ZaC2H2-46</i> and <i>ZaC2H2-55</i> genes were significantly up-regulated under NAA, SA, and MeJA treatments. These results will help to further understand the characteristics of the <i>ZaC2H2</i> gene family and provide a theoretical basis for studying the development of prickles. |
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| ISSN: | 2311-7524 |