Exfoliated Oral Mucosal Cell Micronucleus Frequency Evaluation in Healthy Individuals, Tobacco Users, and Patients with Potentially Malignant Disorders: An Analytical Comparative Study
Objective: Assessing the prevalence of exfoliated oral mucosal cell micronuclei in tobacco smokers, healthy persons, and patients with potentially malignant diseases (PMDs). A critical comparative investigation. Materials and Procedures: The study included 80 participants divided into four groups: h...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2024-12-01
|
| Series: | Journal of Pharmacy and Bioallied Sciences |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/jpbs.jpbs_1074_24 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841543570573492224 |
|---|---|
| author | V Kranthi S Shylaja Pallavi Kandi Mohammed Abidullah Neeharika Soorneedi Tummakomma P. Latha |
| author_facet | V Kranthi S Shylaja Pallavi Kandi Mohammed Abidullah Neeharika Soorneedi Tummakomma P. Latha |
| author_sort | V Kranthi |
| collection | DOAJ |
| description | Objective:
Assessing the prevalence of exfoliated oral mucosal cell micronuclei in tobacco smokers, healthy persons, and patients with potentially malignant diseases (PMDs). A critical comparative investigation.
Materials and Procedures:
The study included 80 participants divided into four groups: healthy persons, tobacco users without any clinically noticeable oral lesions, patients with leukoplakia, and patients with oral submucous fibrosis (OSMF). Each group included 20 participants. Only cases with a verified histological diagnosis of PMDs were considered for evaluating micronuclei (MNi). This included non-smokers with mucosa that seems normal, smokers with mucosa that appears normal, and individuals with leukoplakia and OSMF who were histologically identified.
Results:
Compared to controls, smokers and patients with PMDs had much higher MN frequencies, with PMD patients having the highest MN frequency. There was a noticeable rise in MN frequency in the H and E and PAP stains from controls to tobacco users and then to PMD patients, showing a stepwise development. While the difference between tobacco users and PMD patients was only significant with the H and E stain, the inter-group comparisons show significant differences in MN frequency between controls and both tobacco users and PMD patients across both staining techniques. Additionally, the comparison of the H and E and PAP stains reveals that whereas MN frequencies in smokers and controls are typically similar, PMD patients have considerably higher MN frequencies in the H and E stains.
Conclusion:
In summary, the research indicates that tobacco users have a much higher MN frequency than controls, with an even more pronounced in individuals who have PMDs. |
| format | Article |
| id | doaj-art-d39ffc36b83c46e29a4cb26432cd235e |
| institution | Kabale University |
| issn | 0976-4879 0975-7406 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Pharmacy and Bioallied Sciences |
| spelling | doaj-art-d39ffc36b83c46e29a4cb26432cd235e2025-01-13T10:16:32ZengWolters Kluwer Medknow PublicationsJournal of Pharmacy and Bioallied Sciences0976-48790975-74062024-12-0116Suppl 4S3709S371110.4103/jpbs.jpbs_1074_24Exfoliated Oral Mucosal Cell Micronucleus Frequency Evaluation in Healthy Individuals, Tobacco Users, and Patients with Potentially Malignant Disorders: An Analytical Comparative StudyV KranthiS ShylajaPallavi KandiMohammed AbidullahNeeharika SoorneediTummakomma P. LathaObjective: Assessing the prevalence of exfoliated oral mucosal cell micronuclei in tobacco smokers, healthy persons, and patients with potentially malignant diseases (PMDs). A critical comparative investigation. Materials and Procedures: The study included 80 participants divided into four groups: healthy persons, tobacco users without any clinically noticeable oral lesions, patients with leukoplakia, and patients with oral submucous fibrosis (OSMF). Each group included 20 participants. Only cases with a verified histological diagnosis of PMDs were considered for evaluating micronuclei (MNi). This included non-smokers with mucosa that seems normal, smokers with mucosa that appears normal, and individuals with leukoplakia and OSMF who were histologically identified. Results: Compared to controls, smokers and patients with PMDs had much higher MN frequencies, with PMD patients having the highest MN frequency. There was a noticeable rise in MN frequency in the H and E and PAP stains from controls to tobacco users and then to PMD patients, showing a stepwise development. While the difference between tobacco users and PMD patients was only significant with the H and E stain, the inter-group comparisons show significant differences in MN frequency between controls and both tobacco users and PMD patients across both staining techniques. Additionally, the comparison of the H and E and PAP stains reveals that whereas MN frequencies in smokers and controls are typically similar, PMD patients have considerably higher MN frequencies in the H and E stains. Conclusion: In summary, the research indicates that tobacco users have a much higher MN frequency than controls, with an even more pronounced in individuals who have PMDs.https://journals.lww.com/10.4103/jpbs.jpbs_1074_24exfoliated oral mucosal cellmicronucleus tobacco malignant disordersmnpmd |
| spellingShingle | V Kranthi S Shylaja Pallavi Kandi Mohammed Abidullah Neeharika Soorneedi Tummakomma P. Latha Exfoliated Oral Mucosal Cell Micronucleus Frequency Evaluation in Healthy Individuals, Tobacco Users, and Patients with Potentially Malignant Disorders: An Analytical Comparative Study Journal of Pharmacy and Bioallied Sciences exfoliated oral mucosal cell micronucleus tobacco malignant disorders mn pmd |
| title | Exfoliated Oral Mucosal Cell Micronucleus Frequency Evaluation in Healthy Individuals, Tobacco Users, and Patients with Potentially Malignant Disorders: An Analytical Comparative Study |
| title_full | Exfoliated Oral Mucosal Cell Micronucleus Frequency Evaluation in Healthy Individuals, Tobacco Users, and Patients with Potentially Malignant Disorders: An Analytical Comparative Study |
| title_fullStr | Exfoliated Oral Mucosal Cell Micronucleus Frequency Evaluation in Healthy Individuals, Tobacco Users, and Patients with Potentially Malignant Disorders: An Analytical Comparative Study |
| title_full_unstemmed | Exfoliated Oral Mucosal Cell Micronucleus Frequency Evaluation in Healthy Individuals, Tobacco Users, and Patients with Potentially Malignant Disorders: An Analytical Comparative Study |
| title_short | Exfoliated Oral Mucosal Cell Micronucleus Frequency Evaluation in Healthy Individuals, Tobacco Users, and Patients with Potentially Malignant Disorders: An Analytical Comparative Study |
| title_sort | exfoliated oral mucosal cell micronucleus frequency evaluation in healthy individuals tobacco users and patients with potentially malignant disorders an analytical comparative study |
| topic | exfoliated oral mucosal cell micronucleus tobacco malignant disorders mn pmd |
| url | https://journals.lww.com/10.4103/jpbs.jpbs_1074_24 |
| work_keys_str_mv | AT vkranthi exfoliatedoralmucosalcellmicronucleusfrequencyevaluationinhealthyindividualstobaccousersandpatientswithpotentiallymalignantdisordersananalyticalcomparativestudy AT sshylaja exfoliatedoralmucosalcellmicronucleusfrequencyevaluationinhealthyindividualstobaccousersandpatientswithpotentiallymalignantdisordersananalyticalcomparativestudy AT pallavikandi exfoliatedoralmucosalcellmicronucleusfrequencyevaluationinhealthyindividualstobaccousersandpatientswithpotentiallymalignantdisordersananalyticalcomparativestudy AT mohammedabidullah exfoliatedoralmucosalcellmicronucleusfrequencyevaluationinhealthyindividualstobaccousersandpatientswithpotentiallymalignantdisordersananalyticalcomparativestudy AT neeharikasoorneedi exfoliatedoralmucosalcellmicronucleusfrequencyevaluationinhealthyindividualstobaccousersandpatientswithpotentiallymalignantdisordersananalyticalcomparativestudy AT tummakommaplatha exfoliatedoralmucosalcellmicronucleusfrequencyevaluationinhealthyindividualstobaccousersandpatientswithpotentiallymalignantdisordersananalyticalcomparativestudy |