Prediagnostic Plasma Metabolomic Profiles Using NMR for Exfoliation Glaucoma Among US Health Professionals
Background: Exfoliation glaucoma (XFG) represents a form of deleterious ocular aging of unclear etiology. We evaluated prediagnostic nuclear magnetic resonance (NMR)-based metabolites in relation to XFG risk, expanding on our prior findings of XFG-related metabotypes using liquid chromatography-mass...
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2025-07-01
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| author | Akiko Hanyuda Oana A. Zeleznik Yoshihiko Raita Danielle E. Haslam Qi Sun Kazuno Negishi Louis R. Pasquale Jessica Lasky-Su Janey L. Wiggs Jae H. Kang |
| author_facet | Akiko Hanyuda Oana A. Zeleznik Yoshihiko Raita Danielle E. Haslam Qi Sun Kazuno Negishi Louis R. Pasquale Jessica Lasky-Su Janey L. Wiggs Jae H. Kang |
| author_sort | Akiko Hanyuda |
| collection | DOAJ |
| description | Background: Exfoliation glaucoma (XFG) represents a form of deleterious ocular aging of unclear etiology. We evaluated prediagnostic nuclear magnetic resonance (NMR)-based metabolites in relation to XFG risk, expanding on our prior findings of XFG-related metabotypes using liquid chromatography-mass spectrometry (LC-MS). Methods: We identified 217 XFG cases and 217 matched controls nested within three prospective health professional cohorts with plasma collected a mean 11.8 years before case identification. Plasma metabolites were analyzed using the targeted NMR Nightingale platform. Conditional logistic models and Metabolite Set Enrichment Analysis were performed. Multiple comparison issues were addressed using the number of effective tests (NEF) and false discovery rate (FDR). Results: Among 235 profiled metabolites, higher glucose was significantly associated with a lower risk of XFG (odds ratio (95%CI) = 0.42 (0.26, 0.7); NEF = 0.03). Among metabolite classes, lipoprotein subclasses and branched-chain amino acids were inversely associated, while relative lipoprotein lipid concentrations were adversely associated (FDR < 0.05). Conclusion: NMR profiling revealed that glucose, branched-chain amino acids, lipoprotein subclasses, and relative lipoprotein lipid concentrations may play important roles in XFG etiology. |
| format | Article |
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| institution | Kabale University |
| issn | 2218-1989 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj-art-d3875a41065f41f0a3d8d8d8ce46fb592025-08-20T03:58:27ZengMDPI AGMetabolites2218-19892025-07-0115746910.3390/metabo15070469Prediagnostic Plasma Metabolomic Profiles Using NMR for Exfoliation Glaucoma Among US Health ProfessionalsAkiko Hanyuda0Oana A. Zeleznik1Yoshihiko Raita2Danielle E. Haslam3Qi Sun4Kazuno Negishi5Louis R. Pasquale6Jessica Lasky-Su7Janey L. Wiggs8Jae H. Kang9Department of Ophthalmology, Keio University School of Medicine, Tokyo 160-8582, JapanChanning Division of Network Medicine, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA 02115, USADepartment of Nephrology, Okinawa Prefectural Chubu Hospital, Naha 904-2293, JapanChanning Division of Network Medicine, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA 02115, USAChanning Division of Network Medicine, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA 02115, USADepartment of Ophthalmology, Keio University School of Medicine, Tokyo 160-8582, JapanDepartment of Ophthalmology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USAChanning Division of Network Medicine, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA 02115, USADepartment of Ophthalmology, Harvard Medical School, Massachusetts Eye and Ear Infirmary, Boston, MA 02114, USAChanning Division of Network Medicine, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA 02115, USABackground: Exfoliation glaucoma (XFG) represents a form of deleterious ocular aging of unclear etiology. We evaluated prediagnostic nuclear magnetic resonance (NMR)-based metabolites in relation to XFG risk, expanding on our prior findings of XFG-related metabotypes using liquid chromatography-mass spectrometry (LC-MS). Methods: We identified 217 XFG cases and 217 matched controls nested within three prospective health professional cohorts with plasma collected a mean 11.8 years before case identification. Plasma metabolites were analyzed using the targeted NMR Nightingale platform. Conditional logistic models and Metabolite Set Enrichment Analysis were performed. Multiple comparison issues were addressed using the number of effective tests (NEF) and false discovery rate (FDR). Results: Among 235 profiled metabolites, higher glucose was significantly associated with a lower risk of XFG (odds ratio (95%CI) = 0.42 (0.26, 0.7); NEF = 0.03). Among metabolite classes, lipoprotein subclasses and branched-chain amino acids were inversely associated, while relative lipoprotein lipid concentrations were adversely associated (FDR < 0.05). Conclusion: NMR profiling revealed that glucose, branched-chain amino acids, lipoprotein subclasses, and relative lipoprotein lipid concentrations may play important roles in XFG etiology.https://www.mdpi.com/2218-1989/15/7/469exfoliation glaucomametabolomicsnuclear magnetic resonance spectrometryomics |
| spellingShingle | Akiko Hanyuda Oana A. Zeleznik Yoshihiko Raita Danielle E. Haslam Qi Sun Kazuno Negishi Louis R. Pasquale Jessica Lasky-Su Janey L. Wiggs Jae H. Kang Prediagnostic Plasma Metabolomic Profiles Using NMR for Exfoliation Glaucoma Among US Health Professionals Metabolites exfoliation glaucoma metabolomics nuclear magnetic resonance spectrometry omics |
| title | Prediagnostic Plasma Metabolomic Profiles Using NMR for Exfoliation Glaucoma Among US Health Professionals |
| title_full | Prediagnostic Plasma Metabolomic Profiles Using NMR for Exfoliation Glaucoma Among US Health Professionals |
| title_fullStr | Prediagnostic Plasma Metabolomic Profiles Using NMR for Exfoliation Glaucoma Among US Health Professionals |
| title_full_unstemmed | Prediagnostic Plasma Metabolomic Profiles Using NMR for Exfoliation Glaucoma Among US Health Professionals |
| title_short | Prediagnostic Plasma Metabolomic Profiles Using NMR for Exfoliation Glaucoma Among US Health Professionals |
| title_sort | prediagnostic plasma metabolomic profiles using nmr for exfoliation glaucoma among us health professionals |
| topic | exfoliation glaucoma metabolomics nuclear magnetic resonance spectrometry omics |
| url | https://www.mdpi.com/2218-1989/15/7/469 |
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