Macrophage-derived extracellular vesicles transfer mitochondria to adipocytes and promote adipocyte–myofibroblast transition in epidural fibrosis
Abstract Epidural fibrosis post laminectomy is the leading cause of failed back surgery syndrome. Little is known about the role and mechanisms of adipose tissues in epidural fibrosis. Here, we found that obese patients were more likely to develop epidural fibrosis after spine surgery. Similarly, ob...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-12-01
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| Series: | npj Regenerative Medicine |
| Online Access: | https://doi.org/10.1038/s41536-024-00388-6 |
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| author | Feng Hua Jinpeng Sun Mohan Shi Rui Mei Zeyuan Song Jun Liu Mingshun Zhang |
| author_facet | Feng Hua Jinpeng Sun Mohan Shi Rui Mei Zeyuan Song Jun Liu Mingshun Zhang |
| author_sort | Feng Hua |
| collection | DOAJ |
| description | Abstract Epidural fibrosis post laminectomy is the leading cause of failed back surgery syndrome. Little is known about the role and mechanisms of adipose tissues in epidural fibrosis. Here, we found that obese patients were more likely to develop epidural fibrosis after spine surgery. Similarly, obesity led to more progressive epidural fibrosis in a mouse model of laminectomy. Adipocyte–myofibroblast transition (AMT) occurs in epidural scarring. Mechanistically, large extracellular vesicles (EVs) from M2-type macrophages transfer mitochondria into adipocytes and promote AMT by activating the TGF-β and PAI-1 pathways. Blocking the PAI-1 pathway significantly attenuated the transition of adipocytes into myofibroblasts. We conclude that large EVs from macrophages transfer mitochondria to promote AMT in epidural fibrosis. |
| format | Article |
| id | doaj-art-d376228edecd4d8fa3ef6bd1d140c2e2 |
| institution | Kabale University |
| issn | 2057-3995 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Regenerative Medicine |
| spelling | doaj-art-d376228edecd4d8fa3ef6bd1d140c2e22025-01-05T12:10:50ZengNature Portfolionpj Regenerative Medicine2057-39952024-12-019111410.1038/s41536-024-00388-6Macrophage-derived extracellular vesicles transfer mitochondria to adipocytes and promote adipocyte–myofibroblast transition in epidural fibrosisFeng Hua0Jinpeng Sun1Mohan Shi2Rui Mei3Zeyuan Song4Jun Liu5Mingshun Zhang6Department of Orthopedics, The Second Affiliated Hospital of Nanjing Medical UniversityDepartment of Orthopedics, The Second Affiliated Hospital of Nanjing Medical UniversityDepartment of Orthopedics, The Second Affiliated Hospital of Nanjing Medical UniversityDepartment of Orthopedics, The Second Affiliated Hospital of Nanjing Medical UniversityDepartment of Orthopedics, The Second Affiliated Hospital of Nanjing Medical UniversityDepartment of Orthopedics, The Second Affiliated Hospital of Nanjing Medical UniversityNHC Key Laboratory of Antibody Technique, Jiangsu Province Engineering Research Center of Antibody Drug, Department of Immunology, Nanjing Medical UniversityAbstract Epidural fibrosis post laminectomy is the leading cause of failed back surgery syndrome. Little is known about the role and mechanisms of adipose tissues in epidural fibrosis. Here, we found that obese patients were more likely to develop epidural fibrosis after spine surgery. Similarly, obesity led to more progressive epidural fibrosis in a mouse model of laminectomy. Adipocyte–myofibroblast transition (AMT) occurs in epidural scarring. Mechanistically, large extracellular vesicles (EVs) from M2-type macrophages transfer mitochondria into adipocytes and promote AMT by activating the TGF-β and PAI-1 pathways. Blocking the PAI-1 pathway significantly attenuated the transition of adipocytes into myofibroblasts. We conclude that large EVs from macrophages transfer mitochondria to promote AMT in epidural fibrosis.https://doi.org/10.1038/s41536-024-00388-6 |
| spellingShingle | Feng Hua Jinpeng Sun Mohan Shi Rui Mei Zeyuan Song Jun Liu Mingshun Zhang Macrophage-derived extracellular vesicles transfer mitochondria to adipocytes and promote adipocyte–myofibroblast transition in epidural fibrosis npj Regenerative Medicine |
| title | Macrophage-derived extracellular vesicles transfer mitochondria to adipocytes and promote adipocyte–myofibroblast transition in epidural fibrosis |
| title_full | Macrophage-derived extracellular vesicles transfer mitochondria to adipocytes and promote adipocyte–myofibroblast transition in epidural fibrosis |
| title_fullStr | Macrophage-derived extracellular vesicles transfer mitochondria to adipocytes and promote adipocyte–myofibroblast transition in epidural fibrosis |
| title_full_unstemmed | Macrophage-derived extracellular vesicles transfer mitochondria to adipocytes and promote adipocyte–myofibroblast transition in epidural fibrosis |
| title_short | Macrophage-derived extracellular vesicles transfer mitochondria to adipocytes and promote adipocyte–myofibroblast transition in epidural fibrosis |
| title_sort | macrophage derived extracellular vesicles transfer mitochondria to adipocytes and promote adipocyte myofibroblast transition in epidural fibrosis |
| url | https://doi.org/10.1038/s41536-024-00388-6 |
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