Pro-angiogenic effects of Guo Min decoction in a zebrafish model
Objectives: Guo Min decoction (GMD) is a Chinese traditional medicine that can regulate allergy-related symptoms. Although GMD treatment was reported to treat allergy-associated symptoms by regulating the immune response, the rationale between GMD treatment and angiogenesis has not been reported yet...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2025-01-01
|
| Series: | Tzu-Chi Medical Journal |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/tcmj.tcmj_59_24 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841536598545530880 |
|---|---|
| author | Ping-Hsun Lu Sheng-Fen Tung Chi-Chung Wen Ching-Yuan Huang Jhih-Wei Huang Chih-Hsin Chen Yau-Hung Chen |
| author_facet | Ping-Hsun Lu Sheng-Fen Tung Chi-Chung Wen Ching-Yuan Huang Jhih-Wei Huang Chih-Hsin Chen Yau-Hung Chen |
| author_sort | Ping-Hsun Lu |
| collection | DOAJ |
| description | Objectives:
Guo Min decoction (GMD) is a Chinese traditional medicine that can regulate allergy-related symptoms. Although GMD treatment was reported to treat allergy-associated symptoms by regulating the immune response, the rationale between GMD treatment and angiogenesis has not been reported yet. Our objective is to investigate the angiogenesis-modulating activity of GMD.
Materials and Methods:
In this study, we used fluorescence recording, alkaline phosphatase (AP) activity staining, and real-time polymerase chain reaction (PCR) experiments to examine the effects of GMD on angiogenesis in a zebrafish model.
Results:
GMD-treated zebrafish embryos exhibited more intercapillary spaces in the caudal vein plexus (Mock: 11.1 ± 1.8 [n = 20; n: numbers of embryos]; GMD-treated: 16.2 ± 1.9 [n = 20]). AP activity staining showed that treatment with GMD and liquorice (Gan Cao, a component of GMD) induced subintestinal vein outgrowth. However, glycyrrhizin (a component of Gan Cao) had no obvious pro-angiogenic effects on zebrafish. Furthermore, real-time PCR experiments indicated that GMD exposure might be through regulating angiogenesis-related genes (cdh5, nrp1a, and flt1) expressions.
Conclusion:
Based on these observations, we proposed that GMD had pro-angiogenic activity in a zebrafish model, and it might partially be contributed by one of the components, liquorice. |
| format | Article |
| id | doaj-art-d3631f0ce72c42d2a8d75ddc9afaa82c |
| institution | Kabale University |
| issn | 1016-3190 2223-8956 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Tzu-Chi Medical Journal |
| spelling | doaj-art-d3631f0ce72c42d2a8d75ddc9afaa82c2025-01-14T15:31:19ZengWolters Kluwer Medknow PublicationsTzu-Chi Medical Journal1016-31902223-89562025-01-01371586410.4103/tcmj.tcmj_59_24Pro-angiogenic effects of Guo Min decoction in a zebrafish modelPing-Hsun LuSheng-Fen TungChi-Chung WenChing-Yuan HuangJhih-Wei HuangChih-Hsin ChenYau-Hung ChenObjectives: Guo Min decoction (GMD) is a Chinese traditional medicine that can regulate allergy-related symptoms. Although GMD treatment was reported to treat allergy-associated symptoms by regulating the immune response, the rationale between GMD treatment and angiogenesis has not been reported yet. Our objective is to investigate the angiogenesis-modulating activity of GMD. Materials and Methods: In this study, we used fluorescence recording, alkaline phosphatase (AP) activity staining, and real-time polymerase chain reaction (PCR) experiments to examine the effects of GMD on angiogenesis in a zebrafish model. Results: GMD-treated zebrafish embryos exhibited more intercapillary spaces in the caudal vein plexus (Mock: 11.1 ± 1.8 [n = 20; n: numbers of embryos]; GMD-treated: 16.2 ± 1.9 [n = 20]). AP activity staining showed that treatment with GMD and liquorice (Gan Cao, a component of GMD) induced subintestinal vein outgrowth. However, glycyrrhizin (a component of Gan Cao) had no obvious pro-angiogenic effects on zebrafish. Furthermore, real-time PCR experiments indicated that GMD exposure might be through regulating angiogenesis-related genes (cdh5, nrp1a, and flt1) expressions. Conclusion: Based on these observations, we proposed that GMD had pro-angiogenic activity in a zebrafish model, and it might partially be contributed by one of the components, liquorice.https://journals.lww.com/10.4103/tcmj.tcmj_59_24angiogenesisguo min decoctionreal-time polymerase chain reactionzebrafish |
| spellingShingle | Ping-Hsun Lu Sheng-Fen Tung Chi-Chung Wen Ching-Yuan Huang Jhih-Wei Huang Chih-Hsin Chen Yau-Hung Chen Pro-angiogenic effects of Guo Min decoction in a zebrafish model Tzu-Chi Medical Journal angiogenesis guo min decoction real-time polymerase chain reaction zebrafish |
| title | Pro-angiogenic effects of Guo Min decoction in a zebrafish model |
| title_full | Pro-angiogenic effects of Guo Min decoction in a zebrafish model |
| title_fullStr | Pro-angiogenic effects of Guo Min decoction in a zebrafish model |
| title_full_unstemmed | Pro-angiogenic effects of Guo Min decoction in a zebrafish model |
| title_short | Pro-angiogenic effects of Guo Min decoction in a zebrafish model |
| title_sort | pro angiogenic effects of guo min decoction in a zebrafish model |
| topic | angiogenesis guo min decoction real-time polymerase chain reaction zebrafish |
| url | https://journals.lww.com/10.4103/tcmj.tcmj_59_24 |
| work_keys_str_mv | AT pinghsunlu proangiogeniceffectsofguomindecoctioninazebrafishmodel AT shengfentung proangiogeniceffectsofguomindecoctioninazebrafishmodel AT chichungwen proangiogeniceffectsofguomindecoctioninazebrafishmodel AT chingyuanhuang proangiogeniceffectsofguomindecoctioninazebrafishmodel AT jhihweihuang proangiogeniceffectsofguomindecoctioninazebrafishmodel AT chihhsinchen proangiogeniceffectsofguomindecoctioninazebrafishmodel AT yauhungchen proangiogeniceffectsofguomindecoctioninazebrafishmodel |