Effect Of Different Carbon and Nitrogen Sources on the Activity of Cycloamylase (Cyclomaltodextrin Glucanotransferase) Produced By Sutclifella cohnii
Cyclomaltodextrin glucanotransferase (CGTase) is an important group of microbial amylolytic enzyme that helps in the conversion of starch and other polysaccharides into cyclic oligosaccharides. This study was carried out to determine the effect of alternative carbon and nitrogen sources on the prod...
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Biotechnology Society of Nepal
2024-12-01
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| Series: | Nepal Journal of Biotechnology |
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| Online Access: | https://www.nepjb.com/index.php/NJB/article/view/340 |
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| author | Odunayo Selimot Adedeji Zainab Abdulazeez Oluwafemi Adebayo Oyewole Adamu Yusuf Kabiru Evans Chidi Egwim |
| author_facet | Odunayo Selimot Adedeji Zainab Abdulazeez Oluwafemi Adebayo Oyewole Adamu Yusuf Kabiru Evans Chidi Egwim |
| author_sort | Odunayo Selimot Adedeji |
| collection | DOAJ |
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Cyclomaltodextrin glucanotransferase (CGTase) is an important group of microbial amylolytic enzyme that helps in the conversion of starch and other polysaccharides into cyclic oligosaccharides. This study was carried out to determine the effect of alternative carbon and nitrogen sources on the production of cyclomaltodextrin glucanotransferase from Sutclifella cohni. Rice- soybean water (RS), yam-soybean water (YS), rice-fish water (RF), fufu-fish water (FuF), fufu- soybean water (FuS), rice-meat water (RM), yam-meat water (YM), fufu-meat water (FuM), and yam-fish water (YF) were used as substitute for soluble potato starch in the production medium.. The different carbon and nitrogen sources studied supported the growth and production of CGTase. The optimal pH and temperature was 8.0 and 45oC, respectively. The lowest Km (4.17 mg/mL) was observed with RM as the growth medium. This study concludes that food waste could be used as substitute for soluble starch in the production of CGTase, for industrial application.
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| format | Article |
| id | doaj-art-d32e54850057439fa6c58a1f6d40b837 |
| institution | Kabale University |
| issn | 2091-1130 2467-9313 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Biotechnology Society of Nepal |
| record_format | Article |
| series | Nepal Journal of Biotechnology |
| spelling | doaj-art-d32e54850057439fa6c58a1f6d40b8372025-01-07T14:47:03ZengBiotechnology Society of NepalNepal Journal of Biotechnology2091-11302467-93132024-12-0112210.54796/njb.v12i2.340Effect Of Different Carbon and Nitrogen Sources on the Activity of Cycloamylase (Cyclomaltodextrin Glucanotransferase) Produced By Sutclifella cohniiOdunayo Selimot Adedeji0Zainab Abdulazeez1Oluwafemi Adebayo Oyewole2Adamu Yusuf Kabiru3Evans Chidi Egwim 4Department of Biochemistry, Federal University of Technology, Minna, Niger State, Nigeria; Department of Biochemistry, Federal University Oye-Ekiti, Ekiti State, NigeriaDepartment of Biochemistry, Federal University of Technology, Minna, Niger State, NigeriaDepartment of Biochemistry, Federal University of Technology, Minna, Niger State, NigeriaDepartment of Biochemistry, Federal University of Technology, Minna, Niger State, NigeriaDepartment of Biochemistry, Federal University of Technology, Minna, Niger State, Nigeria; Department of Biochemistry and Chemistry, Caleb University Imota, Lagos State, Nigeria Cyclomaltodextrin glucanotransferase (CGTase) is an important group of microbial amylolytic enzyme that helps in the conversion of starch and other polysaccharides into cyclic oligosaccharides. This study was carried out to determine the effect of alternative carbon and nitrogen sources on the production of cyclomaltodextrin glucanotransferase from Sutclifella cohni. Rice- soybean water (RS), yam-soybean water (YS), rice-fish water (RF), fufu-fish water (FuF), fufu- soybean water (FuS), rice-meat water (RM), yam-meat water (YM), fufu-meat water (FuM), and yam-fish water (YF) were used as substitute for soluble potato starch in the production medium.. The different carbon and nitrogen sources studied supported the growth and production of CGTase. The optimal pH and temperature was 8.0 and 45oC, respectively. The lowest Km (4.17 mg/mL) was observed with RM as the growth medium. This study concludes that food waste could be used as substitute for soluble starch in the production of CGTase, for industrial application. https://www.nepjb.com/index.php/NJB/article/view/340BiotechnologyCarbonCyclomaltodextrin glucanotransferaseIndustrialNitrogen |
| spellingShingle | Odunayo Selimot Adedeji Zainab Abdulazeez Oluwafemi Adebayo Oyewole Adamu Yusuf Kabiru Evans Chidi Egwim Effect Of Different Carbon and Nitrogen Sources on the Activity of Cycloamylase (Cyclomaltodextrin Glucanotransferase) Produced By Sutclifella cohnii Nepal Journal of Biotechnology Biotechnology Carbon Cyclomaltodextrin glucanotransferase Industrial Nitrogen |
| title | Effect Of Different Carbon and Nitrogen Sources on the Activity of Cycloamylase (Cyclomaltodextrin Glucanotransferase) Produced By Sutclifella cohnii |
| title_full | Effect Of Different Carbon and Nitrogen Sources on the Activity of Cycloamylase (Cyclomaltodextrin Glucanotransferase) Produced By Sutclifella cohnii |
| title_fullStr | Effect Of Different Carbon and Nitrogen Sources on the Activity of Cycloamylase (Cyclomaltodextrin Glucanotransferase) Produced By Sutclifella cohnii |
| title_full_unstemmed | Effect Of Different Carbon and Nitrogen Sources on the Activity of Cycloamylase (Cyclomaltodextrin Glucanotransferase) Produced By Sutclifella cohnii |
| title_short | Effect Of Different Carbon and Nitrogen Sources on the Activity of Cycloamylase (Cyclomaltodextrin Glucanotransferase) Produced By Sutclifella cohnii |
| title_sort | effect of different carbon and nitrogen sources on the activity of cycloamylase cyclomaltodextrin glucanotransferase produced by sutclifella cohnii |
| topic | Biotechnology Carbon Cyclomaltodextrin glucanotransferase Industrial Nitrogen |
| url | https://www.nepjb.com/index.php/NJB/article/view/340 |
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