Cellular Characterization and Interspecies Evolution of the Tree Shrew Retina across Postnatal Lifespan
Tree shrews (TSs) possess a highly developed visual system. Here, we establish an age-related single-cell RNA sequencing atlas of retina cells from 15 TSs, covering 6 major retina cell classes and 3 glial cell types. An age effect is observed on the cell subset composition and gene expression patter...
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| Language: | English |
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American Association for the Advancement of Science (AAAS)
2024-01-01
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| Series: | Research |
| Online Access: | https://spj.science.org/doi/10.34133/research.0536 |
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| author | Liu-Lin Xiong Yi-Fei Sun Rui-Ze Niu Lu-Lu Xue Li Chen Li-Ren Huangfu Jing Li Yu-Ying Wang Xin Liu Wen-Yuan Wang Zhong-Fu Zuo Ting-Hua Wang |
| author_facet | Liu-Lin Xiong Yi-Fei Sun Rui-Ze Niu Lu-Lu Xue Li Chen Li-Ren Huangfu Jing Li Yu-Ying Wang Xin Liu Wen-Yuan Wang Zhong-Fu Zuo Ting-Hua Wang |
| author_sort | Liu-Lin Xiong |
| collection | DOAJ |
| description | Tree shrews (TSs) possess a highly developed visual system. Here, we establish an age-related single-cell RNA sequencing atlas of retina cells from 15 TSs, covering 6 major retina cell classes and 3 glial cell types. An age effect is observed on the cell subset composition and gene expression pattern. We then verify the cell subtypes and identify specific markers in the TS retina including CA10 for bipolar cells, MEGF11 for H1 horizontal cells, and SLIT2, RUNX1, FOXP2, and SPP1 for retinal ganglion cell subpopulations. The cross-species analysis elucidates the cell type-specific transcriptional programs, different cell compositions, and cell communications. The comparisons also reveal that TS cones and subclasses of bipolar and amacrine cells exhibit the closest relationship with humans and macaques. Our results suggests that TS could be used as a better disease model to understand age-dependent cellular and genetic mechanisms of the retina, particularly for the retinal diseases associated with cones. |
| format | Article |
| id | doaj-art-d31b47c4586b42e087b0e5b03acbfc44 |
| institution | Kabale University |
| issn | 2639-5274 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | Research |
| spelling | doaj-art-d31b47c4586b42e087b0e5b03acbfc442024-11-21T09:00:18ZengAmerican Association for the Advancement of Science (AAAS)Research2639-52742024-01-01710.34133/research.0536Cellular Characterization and Interspecies Evolution of the Tree Shrew Retina across Postnatal LifespanLiu-Lin Xiong0Yi-Fei Sun1Rui-Ze Niu2Lu-Lu Xue3Li Chen4Li-Ren Huangfu5Jing Li6Yu-Ying Wang7Xin Liu8Wen-Yuan Wang9Zhong-Fu Zuo10Ting-Hua Wang11Department of Anesthesiology, Research Institute of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China.Department of Urology, the Second Affiliated Hospital of Kunming Medical University, Kunming 650500, China.Mental Health Center of Kunming Medical University, Kunming 650034, Yunnan, China.State Key Lab of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China.Department of Anesthesiology, Research Institute of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China.Institute of Neuroscience, Kunming Medical University, Kunming 650500, Yunnan, China.Institute of Neuroscience, Kunming Medical University, Kunming 650500, Yunnan, China.Department of Anatomy, College of Basic Medicine, Jinzhou Medical University, Jinzhou 121001, Liaoning, China.Department of Anatomy, College of Basic Medicine, Jinzhou Medical University, Jinzhou 121001, Liaoning, China.Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Science, Shanghai 200032, China.Department of Anatomy, College of Basic Medicine, Jinzhou Medical University, Jinzhou 121001, Liaoning, China.Department of Anesthesiology, Research Institute of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China.Tree shrews (TSs) possess a highly developed visual system. Here, we establish an age-related single-cell RNA sequencing atlas of retina cells from 15 TSs, covering 6 major retina cell classes and 3 glial cell types. An age effect is observed on the cell subset composition and gene expression pattern. We then verify the cell subtypes and identify specific markers in the TS retina including CA10 for bipolar cells, MEGF11 for H1 horizontal cells, and SLIT2, RUNX1, FOXP2, and SPP1 for retinal ganglion cell subpopulations. The cross-species analysis elucidates the cell type-specific transcriptional programs, different cell compositions, and cell communications. The comparisons also reveal that TS cones and subclasses of bipolar and amacrine cells exhibit the closest relationship with humans and macaques. Our results suggests that TS could be used as a better disease model to understand age-dependent cellular and genetic mechanisms of the retina, particularly for the retinal diseases associated with cones.https://spj.science.org/doi/10.34133/research.0536 |
| spellingShingle | Liu-Lin Xiong Yi-Fei Sun Rui-Ze Niu Lu-Lu Xue Li Chen Li-Ren Huangfu Jing Li Yu-Ying Wang Xin Liu Wen-Yuan Wang Zhong-Fu Zuo Ting-Hua Wang Cellular Characterization and Interspecies Evolution of the Tree Shrew Retina across Postnatal Lifespan Research |
| title | Cellular Characterization and Interspecies Evolution of the Tree Shrew Retina across Postnatal Lifespan |
| title_full | Cellular Characterization and Interspecies Evolution of the Tree Shrew Retina across Postnatal Lifespan |
| title_fullStr | Cellular Characterization and Interspecies Evolution of the Tree Shrew Retina across Postnatal Lifespan |
| title_full_unstemmed | Cellular Characterization and Interspecies Evolution of the Tree Shrew Retina across Postnatal Lifespan |
| title_short | Cellular Characterization and Interspecies Evolution of the Tree Shrew Retina across Postnatal Lifespan |
| title_sort | cellular characterization and interspecies evolution of the tree shrew retina across postnatal lifespan |
| url | https://spj.science.org/doi/10.34133/research.0536 |
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