Optimized laboratory techniques for assessing the quality of pre-stripped DMEK grafts

Optimized laboratory techniques for assessing the quality of pre-stripped DMEK grafts

Abstract This study addressed limitations in calcein-AM-based endothelial viability assays, specifically focusing on pre-stripped DMEK grafts. Key challenges included the suboptimal calcein staining and the incompatibility of the viability assay with subsequent immunofluorescence (IF). Using human c...

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Main Authors: Tomy Sagnial, Sandrine Ninotta, Paul Goin, Inès Aouimeur, Louise Parveau, Sylvain Poinard, Oliver Dorado Cortez, Olfa Ben Moussa, Hanielle Vaitinadapoule, Anne-Sophie Gauthier, Philippe Gain, Gilles Thuret, Zhiguo He
Format: Article
Language:English
Published: Nature Portfolio 2025-03-01
Series:Scientific Reports
Subjects:
Pre-stripped DMEK grafts
Eye bank
Endothelial viability
Calcein-AM
Endothelial cell density (ECD)
Viable ECD
Online Access:https://doi.org/10.1038/s41598-025-91512-z
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https://doi.org/10.1038/s41598-025-91512-z

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