Identification and Gene Fine Mapping of the Bisexual Sterility Mutant <i>Meiosis Abnormal Bisexual Sterility 1</i> in Rice
Exploring the genes regulating rice fertility is of great value for studying the molecular mechanisms of rice reproductive development and production practices. In this study, we identified a sterile mutant from the mutant library induced by ethyl methanesulfonate (EMS), designated as <i>meios...
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2024-11-01
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| author | Yingchun Wan Xiaoqing Liu Nan Wang Zhengming Zeng Yudong Jiang |
| author_facet | Yingchun Wan Xiaoqing Liu Nan Wang Zhengming Zeng Yudong Jiang |
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| description | Exploring the genes regulating rice fertility is of great value for studying the molecular mechanisms of rice reproductive development and production practices. In this study, we identified a sterile mutant from the mutant library induced by ethyl methanesulfonate (EMS), designated as <i>meiosis abnormal bisexual sterility 1</i> (<i>mabs1</i>). The <i>mabs1</i> mutant exhibits no phenotypic differences from the wild-type during the vegetative growth phase but shows complete sterility during the reproductive growth phase. Phenotypic observations revealed that both pollen and embryo sac fertility are lost in <i>mabs1</i>. Notably, in <i>mabs1</i>, the development of the anther inner and outer walls, tapetum degeneration, and callose synthesis and degradation all proceed normally, yet meiosis fails to form normal tetrads. Genetic analysis indicated that this mutant trait is controlled by a single recessive nuclear gene. By constructing a genetic segregation population, we successfully mapped the <i>MABS1</i> gene to a 49 kb region between primer markers Y7 and Y9 on chromosome 1. Resequencing revealed a single-nucleotide substitution in the exon of the <i>LOC_Os01g66170</i> gene, which resulted in a change from Valine to Isoleucine. Subsequent sequencing of this locus in both wild-type and <i>mabs1</i> mutants confirmed this mutation. Therefore, we have identified the gene at <i>LOC_Os01g66170</i> as a candidate for <i>MABS1</i>, a previously unreported novel gene involved in rice meiosis. Through RT-qPCR, we found that the expression levels of multiple meiosis-related genes were significantly changed in the <i>mabs1</i> mutant. Therefore, we believe that <i>MABS1</i> is also involved in the process of rice meiosis. This study lays the groundwork for a functional study of <i>MABS1</i>. |
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| spelling | doaj-art-d2f28f1a65484ce7bcce2e664a5fb9b22024-11-26T17:58:05ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452024-11-014611129781299310.3390/cimb46110773Identification and Gene Fine Mapping of the Bisexual Sterility Mutant <i>Meiosis Abnormal Bisexual Sterility 1</i> in RiceYingchun Wan0Xiaoqing Liu1Nan Wang2Zhengming Zeng3Yudong Jiang4Deyang Branch of Sichuan Academy of Agricultural Sciences, Luzhou Branch of National Rice Improvement Center, Southwest Key Laboratory of Rice Biology, Rice and Sorghum, Research Institute of Sichuan Academy of Agricultural Sciences, Genetics and Breeding of Ministry of Agriculture, Deyang 618000, China Transgenic Plants and Safety Control Chongqing Municipal Key Laboratory, Rice Research Institute of Southwest University, Engineering Research Center of Southern Mountain Agriculture Ministry of Education, Chongqing 400716, China Transgenic Plants and Safety Control Chongqing Municipal Key Laboratory, Rice Research Institute of Southwest University, Engineering Research Center of Southern Mountain Agriculture Ministry of Education, Chongqing 400716, China Deyang Branch of Sichuan Academy of Agricultural Sciences, Luzhou Branch of National Rice Improvement Center, Southwest Key Laboratory of Rice Biology, Rice and Sorghum, Research Institute of Sichuan Academy of Agricultural Sciences, Genetics and Breeding of Ministry of Agriculture, Deyang 618000, China Deyang Branch of Sichuan Academy of Agricultural Sciences, Luzhou Branch of National Rice Improvement Center, Southwest Key Laboratory of Rice Biology, Rice and Sorghum, Research Institute of Sichuan Academy of Agricultural Sciences, Genetics and Breeding of Ministry of Agriculture, Deyang 618000, China Exploring the genes regulating rice fertility is of great value for studying the molecular mechanisms of rice reproductive development and production practices. In this study, we identified a sterile mutant from the mutant library induced by ethyl methanesulfonate (EMS), designated as <i>meiosis abnormal bisexual sterility 1</i> (<i>mabs1</i>). The <i>mabs1</i> mutant exhibits no phenotypic differences from the wild-type during the vegetative growth phase but shows complete sterility during the reproductive growth phase. Phenotypic observations revealed that both pollen and embryo sac fertility are lost in <i>mabs1</i>. Notably, in <i>mabs1</i>, the development of the anther inner and outer walls, tapetum degeneration, and callose synthesis and degradation all proceed normally, yet meiosis fails to form normal tetrads. Genetic analysis indicated that this mutant trait is controlled by a single recessive nuclear gene. By constructing a genetic segregation population, we successfully mapped the <i>MABS1</i> gene to a 49 kb region between primer markers Y7 and Y9 on chromosome 1. Resequencing revealed a single-nucleotide substitution in the exon of the <i>LOC_Os01g66170</i> gene, which resulted in a change from Valine to Isoleucine. Subsequent sequencing of this locus in both wild-type and <i>mabs1</i> mutants confirmed this mutation. Therefore, we have identified the gene at <i>LOC_Os01g66170</i> as a candidate for <i>MABS1</i>, a previously unreported novel gene involved in rice meiosis. Through RT-qPCR, we found that the expression levels of multiple meiosis-related genes were significantly changed in the <i>mabs1</i> mutant. Therefore, we believe that <i>MABS1</i> is also involved in the process of rice meiosis. This study lays the groundwork for a functional study of <i>MABS1</i>.https://www.mdpi.com/1467-3045/46/11/773rice (<i>Oryza sativa</i> L.)bisexual sterilitymeiosisgene mapping<i>MABS1</i> |
| spellingShingle | Yingchun Wan Xiaoqing Liu Nan Wang Zhengming Zeng Yudong Jiang Identification and Gene Fine Mapping of the Bisexual Sterility Mutant <i>Meiosis Abnormal Bisexual Sterility 1</i> in Rice Current Issues in Molecular Biology rice (<i>Oryza sativa</i> L.) bisexual sterility meiosis gene mapping <i>MABS1</i> |
| title | Identification and Gene Fine Mapping of the Bisexual Sterility Mutant <i>Meiosis Abnormal Bisexual Sterility 1</i> in Rice |
| title_full | Identification and Gene Fine Mapping of the Bisexual Sterility Mutant <i>Meiosis Abnormal Bisexual Sterility 1</i> in Rice |
| title_fullStr | Identification and Gene Fine Mapping of the Bisexual Sterility Mutant <i>Meiosis Abnormal Bisexual Sterility 1</i> in Rice |
| title_full_unstemmed | Identification and Gene Fine Mapping of the Bisexual Sterility Mutant <i>Meiosis Abnormal Bisexual Sterility 1</i> in Rice |
| title_short | Identification and Gene Fine Mapping of the Bisexual Sterility Mutant <i>Meiosis Abnormal Bisexual Sterility 1</i> in Rice |
| title_sort | identification and gene fine mapping of the bisexual sterility mutant i meiosis abnormal bisexual sterility 1 i in rice |
| topic | rice (<i>Oryza sativa</i> L.) bisexual sterility meiosis gene mapping <i>MABS1</i> |
| url | https://www.mdpi.com/1467-3045/46/11/773 |
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