Peroxidase (POD) Mimicking Activity of Different Types of Poly(ethyleneimine)-Mediated Prussian Blue Nanoparticles
Prussian blue nanoparticles (PBNPs) have been identified as a promising candidate for biomimetic peroxidase (POD)-like activity, specifically due to the metal centres (Fe<sup>3+</sup>/Fe<sup>2+</sup>) of Prussian blue (PB), which have the potential to function as catalyticall...
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2024-12-01
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| author | Udara Bimendra Gunatilake Briza Pérez-López Maria Urpi Judit Prat-Trunas Gerard Carrera-Cardona Gautier Félix Saad Sene Mickaël Beaudhuin Jean-Charles Dupin Joachim Allouche Yannick Guari Joulia Larionova Eva Baldrich |
| author_facet | Udara Bimendra Gunatilake Briza Pérez-López Maria Urpi Judit Prat-Trunas Gerard Carrera-Cardona Gautier Félix Saad Sene Mickaël Beaudhuin Jean-Charles Dupin Joachim Allouche Yannick Guari Joulia Larionova Eva Baldrich |
| author_sort | Udara Bimendra Gunatilake |
| collection | DOAJ |
| description | Prussian blue nanoparticles (PBNPs) have been identified as a promising candidate for biomimetic peroxidase (POD)-like activity, specifically due to the metal centres (Fe<sup>3+</sup>/Fe<sup>2+</sup>) of Prussian blue (PB), which have the potential to function as catalytically active centres. The decoration of PBNPs with desired functional polymers (such as amino- or carboxylate-based) primarily facilitates the subsequent linkage of biomolecules to the nanoparticles for their use in biosensor applications. Thus, the elucidation of the catalytic POD mimicry of these systems is of significant scientific interest but has not been investigated in depth yet. In this report, we studied a series of poly(ethyleneimine) (PEI)-mediated PBNPs (PB/PEI NPs) prepared using various synthesis protocols. The resulting range of particles with varying size (~19–92 nm) and shape combinations were characterised in order to gain insights into their physicochemical properties. The POD-like nanozyme activity of these nanoparticles was then investigated by utilising a 3,3′,5,5′-tetramethylbenzidine (TMB)/H<sub>2</sub>O<sub>2</sub> system, with the catalytic performance of the natural enzyme horseradish peroxidase (HRP) serving as a point of comparison. It was shown that most PB/PEI NPs displayed higher catalytic activity than the PBNPs, with higher activity observed in particles of smaller size, higher Fe content, and higher Fe<sup>2+</sup>/Fe<sup>3+</sup> ratio. Furthermore, the nanoparticles demonstrated enhanced chemical stability in the presence of acid, sodium azide, or high concentrations of H<sub>2</sub>O<sub>2</sub> when compared to HRP, confirming the viability of PB/PEI NPs as a promising nanozymatic material. This study disseminates fundamental knowledge on PB/PEI NPs and their POD-like activities, which will facilitate the selection of an appropriate particle type for future biosensor applications. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2024-12-01 |
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| series | Nanomaterials |
| spelling | doaj-art-d2cf8ff4416a40ca9ec9a479362d19c92025-01-10T13:19:20ZengMDPI AGNanomaterials2079-49912024-12-011514110.3390/nano15010041Peroxidase (POD) Mimicking Activity of Different Types of Poly(ethyleneimine)-Mediated Prussian Blue NanoparticlesUdara Bimendra Gunatilake0Briza Pérez-López1Maria Urpi2Judit Prat-Trunas3Gerard Carrera-Cardona4Gautier Félix5Saad Sene6Mickaël Beaudhuin7Jean-Charles Dupin8Joachim Allouche9Yannick Guari10Joulia Larionova11Eva Baldrich12ICGM, University of Montpellier, CNRS, ENSCM, 34000 Montpellier, FranceDiagnostic Nanotools Group, Hospital Vall d’Hebron Institut de Recerca (VHIR), 08035 Barcelona, SpainDiagnostic Nanotools Group, Hospital Vall d’Hebron Institut de Recerca (VHIR), 08035 Barcelona, SpainDiagnostic Nanotools Group, Hospital Vall d’Hebron Institut de Recerca (VHIR), 08035 Barcelona, SpainDiagnostic Nanotools Group, Hospital Vall d’Hebron Institut de Recerca (VHIR), 08035 Barcelona, SpainICGM, University of Montpellier, CNRS, ENSCM, 34000 Montpellier, FranceICGM, University of Montpellier, CNRS, ENSCM, 34000 Montpellier, FranceICGM, University of Montpellier, CNRS, ENSCM, 34000 Montpellier, FranceInstitut des Sciences Analytiques et de Physicochimie Pour l’Environnement et les Matériaux, UMR 5254, E2S UPPA, CNRS, IPREM, 64000 Pau, FranceInstitut des Sciences Analytiques et de Physicochimie Pour l’Environnement et les Matériaux, UMR 5254, E2S UPPA, CNRS, IPREM, 64000 Pau, FranceICGM, University of Montpellier, CNRS, ENSCM, 34000 Montpellier, FranceICGM, University of Montpellier, CNRS, ENSCM, 34000 Montpellier, FranceDiagnostic Nanotools Group, Hospital Vall d’Hebron Institut de Recerca (VHIR), 08035 Barcelona, SpainPrussian blue nanoparticles (PBNPs) have been identified as a promising candidate for biomimetic peroxidase (POD)-like activity, specifically due to the metal centres (Fe<sup>3+</sup>/Fe<sup>2+</sup>) of Prussian blue (PB), which have the potential to function as catalytically active centres. The decoration of PBNPs with desired functional polymers (such as amino- or carboxylate-based) primarily facilitates the subsequent linkage of biomolecules to the nanoparticles for their use in biosensor applications. Thus, the elucidation of the catalytic POD mimicry of these systems is of significant scientific interest but has not been investigated in depth yet. In this report, we studied a series of poly(ethyleneimine) (PEI)-mediated PBNPs (PB/PEI NPs) prepared using various synthesis protocols. The resulting range of particles with varying size (~19–92 nm) and shape combinations were characterised in order to gain insights into their physicochemical properties. The POD-like nanozyme activity of these nanoparticles was then investigated by utilising a 3,3′,5,5′-tetramethylbenzidine (TMB)/H<sub>2</sub>O<sub>2</sub> system, with the catalytic performance of the natural enzyme horseradish peroxidase (HRP) serving as a point of comparison. It was shown that most PB/PEI NPs displayed higher catalytic activity than the PBNPs, with higher activity observed in particles of smaller size, higher Fe content, and higher Fe<sup>2+</sup>/Fe<sup>3+</sup> ratio. Furthermore, the nanoparticles demonstrated enhanced chemical stability in the presence of acid, sodium azide, or high concentrations of H<sub>2</sub>O<sub>2</sub> when compared to HRP, confirming the viability of PB/PEI NPs as a promising nanozymatic material. This study disseminates fundamental knowledge on PB/PEI NPs and their POD-like activities, which will facilitate the selection of an appropriate particle type for future biosensor applications.https://www.mdpi.com/2079-4991/15/1/41Prussian blue nanoparticles (PBNPs)nanozymepoly(ethyleneimine) (PEI)peroxidase mimeticamino-rich polymerscatalysts |
| spellingShingle | Udara Bimendra Gunatilake Briza Pérez-López Maria Urpi Judit Prat-Trunas Gerard Carrera-Cardona Gautier Félix Saad Sene Mickaël Beaudhuin Jean-Charles Dupin Joachim Allouche Yannick Guari Joulia Larionova Eva Baldrich Peroxidase (POD) Mimicking Activity of Different Types of Poly(ethyleneimine)-Mediated Prussian Blue Nanoparticles Nanomaterials Prussian blue nanoparticles (PBNPs) nanozyme poly(ethyleneimine) (PEI) peroxidase mimetic amino-rich polymers catalysts |
| title | Peroxidase (POD) Mimicking Activity of Different Types of Poly(ethyleneimine)-Mediated Prussian Blue Nanoparticles |
| title_full | Peroxidase (POD) Mimicking Activity of Different Types of Poly(ethyleneimine)-Mediated Prussian Blue Nanoparticles |
| title_fullStr | Peroxidase (POD) Mimicking Activity of Different Types of Poly(ethyleneimine)-Mediated Prussian Blue Nanoparticles |
| title_full_unstemmed | Peroxidase (POD) Mimicking Activity of Different Types of Poly(ethyleneimine)-Mediated Prussian Blue Nanoparticles |
| title_short | Peroxidase (POD) Mimicking Activity of Different Types of Poly(ethyleneimine)-Mediated Prussian Blue Nanoparticles |
| title_sort | peroxidase pod mimicking activity of different types of poly ethyleneimine mediated prussian blue nanoparticles |
| topic | Prussian blue nanoparticles (PBNPs) nanozyme poly(ethyleneimine) (PEI) peroxidase mimetic amino-rich polymers catalysts |
| url | https://www.mdpi.com/2079-4991/15/1/41 |
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