MHC-1B carried exosomes derived from tubular epithelial cell induced by the EGFR mimotope inhibit macrophage activation in renal fibrosis
The high prevalence of chronic kidney disease (CKD) is a significant public health burden worldwide. Renal fibrosis is an inevitable pathological process in almost all CKD cases. To date, the available treatments to prevent or delay renal fibrosis progression are limited. Epidermal growth factor rec...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
|
| Series: | Extracellular Vesicle |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2773041723000033 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846159424633176064 |
|---|---|
| author | Jin Guo Xuanqi Liu Haoming Song Yong Gu Jianying Niu Lin Yang |
| author_facet | Jin Guo Xuanqi Liu Haoming Song Yong Gu Jianying Niu Lin Yang |
| author_sort | Jin Guo |
| collection | DOAJ |
| description | The high prevalence of chronic kidney disease (CKD) is a significant public health burden worldwide. Renal fibrosis is an inevitable pathological process in almost all CKD cases. To date, the available treatments to prevent or delay renal fibrosis progression are limited. Epidermal growth factor receptor (EGFR) mimotope has been demonstrated to alleviate renal fibrosis in the unilateral ureteric obstruction (UUO) model as a type of vaccine. Further observations suggested that the EGFR mimotope can inhibit the activation of macrophages, which play a vital role in renal fibrosis. In the current study, we explored the link triggered by the EGFR mimotopes between tubular epithelial cells (TECs) and macrophages. A series of experiments indicated that EGFR mimotope immunization can inhibit the activation of THP-1 macrophages by TEC-derived exosomes. Injection of these exosomes ameliorated renal fibrosis in the UUO model. Mechanism analysis suggested that the major histocompatibility complex-1B (MHC-1B) was the key molecule in the TECs exosomes induced by EGFR mimotope immunization and is regulated by ribosomal protein L6 (RPL-6). This study revealed a new specific working path of the EGFR mimotope and suggests that the application of the EGFR mimotope can be extended to other immune-related disorders. |
| format | Article |
| id | doaj-art-d29ca3f76dc94c4e84e1d73a0b7e6dc0 |
| institution | Kabale University |
| issn | 2773-0417 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Extracellular Vesicle |
| spelling | doaj-art-d29ca3f76dc94c4e84e1d73a0b7e6dc02024-11-23T06:34:52ZengElsevierExtracellular Vesicle2773-04172023-12-012100024MHC-1B carried exosomes derived from tubular epithelial cell induced by the EGFR mimotope inhibit macrophage activation in renal fibrosisJin Guo0Xuanqi Liu1Haoming Song2Yong Gu3Jianying Niu4Lin Yang5Department of Cardiorespiratory Rehabilitation, Shanghai Yangzhi Rehabilitation Hospital (Shanghai Sunshine Rehabilitation Center), Tongji University School of Medicine, No. 2209, Guangxing Road, Shanghai, ChinaDepartment of Rheumatology and Immunology, Nanjing Drum Tower Hospital Clinical College of Nanjing University of Chinese Medicine, No. 321, Zhongshan Road, Nanjing, ChinaDepartment of Cardiology, Tongji Hospital, Tongji University School of Medicine, No. 389, Xincun Road, Shanghai, ChinaDepartment of Nephrology, Shanghai Fifth People’s Hospital, Fudan University, No. 801, Heqing Road, Shanghai, ChinaDepartment of Nephrology, Shanghai Fifth People’s Hospital, Fudan University, No. 801, Heqing Road, Shanghai, China; Corresponding author at: Department of Nephrology, Shanghai Fifth People’s Hospital, Fudan University, No. 801, Heqing Road, Shanghai 200240, China.Department of Nephrology, Shanghai Fifth People’s Hospital, Fudan University, No. 801, Heqing Road, Shanghai, China; Corresponding author at: Department of Nephrology, Shanghai Fifth People’s Hospital, Fudan University, No. 801, Heqing Road, Shanghai 200240, China.The high prevalence of chronic kidney disease (CKD) is a significant public health burden worldwide. Renal fibrosis is an inevitable pathological process in almost all CKD cases. To date, the available treatments to prevent or delay renal fibrosis progression are limited. Epidermal growth factor receptor (EGFR) mimotope has been demonstrated to alleviate renal fibrosis in the unilateral ureteric obstruction (UUO) model as a type of vaccine. Further observations suggested that the EGFR mimotope can inhibit the activation of macrophages, which play a vital role in renal fibrosis. In the current study, we explored the link triggered by the EGFR mimotopes between tubular epithelial cells (TECs) and macrophages. A series of experiments indicated that EGFR mimotope immunization can inhibit the activation of THP-1 macrophages by TEC-derived exosomes. Injection of these exosomes ameliorated renal fibrosis in the UUO model. Mechanism analysis suggested that the major histocompatibility complex-1B (MHC-1B) was the key molecule in the TECs exosomes induced by EGFR mimotope immunization and is regulated by ribosomal protein L6 (RPL-6). This study revealed a new specific working path of the EGFR mimotope and suggests that the application of the EGFR mimotope can be extended to other immune-related disorders.http://www.sciencedirect.com/science/article/pii/S2773041723000033Renal fibrosisMimotopeExosomeRibosomal protein L6Major histocompatibility complex-1B |
| spellingShingle | Jin Guo Xuanqi Liu Haoming Song Yong Gu Jianying Niu Lin Yang MHC-1B carried exosomes derived from tubular epithelial cell induced by the EGFR mimotope inhibit macrophage activation in renal fibrosis Extracellular Vesicle Renal fibrosis Mimotope Exosome Ribosomal protein L6 Major histocompatibility complex-1B |
| title | MHC-1B carried exosomes derived from tubular epithelial cell induced by the EGFR mimotope inhibit macrophage activation in renal fibrosis |
| title_full | MHC-1B carried exosomes derived from tubular epithelial cell induced by the EGFR mimotope inhibit macrophage activation in renal fibrosis |
| title_fullStr | MHC-1B carried exosomes derived from tubular epithelial cell induced by the EGFR mimotope inhibit macrophage activation in renal fibrosis |
| title_full_unstemmed | MHC-1B carried exosomes derived from tubular epithelial cell induced by the EGFR mimotope inhibit macrophage activation in renal fibrosis |
| title_short | MHC-1B carried exosomes derived from tubular epithelial cell induced by the EGFR mimotope inhibit macrophage activation in renal fibrosis |
| title_sort | mhc 1b carried exosomes derived from tubular epithelial cell induced by the egfr mimotope inhibit macrophage activation in renal fibrosis |
| topic | Renal fibrosis Mimotope Exosome Ribosomal protein L6 Major histocompatibility complex-1B |
| url | http://www.sciencedirect.com/science/article/pii/S2773041723000033 |
| work_keys_str_mv | AT jinguo mhc1bcarriedexosomesderivedfromtubularepithelialcellinducedbytheegfrmimotopeinhibitmacrophageactivationinrenalfibrosis AT xuanqiliu mhc1bcarriedexosomesderivedfromtubularepithelialcellinducedbytheegfrmimotopeinhibitmacrophageactivationinrenalfibrosis AT haomingsong mhc1bcarriedexosomesderivedfromtubularepithelialcellinducedbytheegfrmimotopeinhibitmacrophageactivationinrenalfibrosis AT yonggu mhc1bcarriedexosomesderivedfromtubularepithelialcellinducedbytheegfrmimotopeinhibitmacrophageactivationinrenalfibrosis AT jianyingniu mhc1bcarriedexosomesderivedfromtubularepithelialcellinducedbytheegfrmimotopeinhibitmacrophageactivationinrenalfibrosis AT linyang mhc1bcarriedexosomesderivedfromtubularepithelialcellinducedbytheegfrmimotopeinhibitmacrophageactivationinrenalfibrosis |