Raffinose Priming Improves Seed Vigor by ROS Scavenging, RAFS, and α-GAL Activity in Aged Waxy Corn
Raffinose family oligosaccharides (RFOs) are known to benefit plants under stress conditions; however, the role of exogenous raffinose in seed germination remains poorly understood. In this study, we investigated the potential role of raffinose in promoting seed germination and elucidated the underl...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/14/12/2843 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846106373851447296 |
|---|---|
| author | Min Zhu Ru Xiao Tong Yu Tao Guo Xuemei Zhong Jianzhou Qu Wanli Du Wei Xue |
| author_facet | Min Zhu Ru Xiao Tong Yu Tao Guo Xuemei Zhong Jianzhou Qu Wanli Du Wei Xue |
| author_sort | Min Zhu |
| collection | DOAJ |
| description | Raffinose family oligosaccharides (RFOs) are known to benefit plants under stress conditions; however, the role of exogenous raffinose in seed germination remains poorly understood. In this study, we investigated the potential role of raffinose in promoting seed germination and elucidated the underlying mechanisms. The results showed that artificial aging significantly reduced the germination rate and vigor of waxy corn seeds. Conversely, exogenous raffinose significantly enhanced the germination of these artificially aged seeds. Exogenous raffinose significantly reduced the levels of reactive oxygen species (O<sub>2</sub><sup>−</sup> and H<sub>2</sub>O<sub>2</sub>) and enhanced the activity of antioxidant enzymes, including superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT). Additionally, the levels of α-galactosidase (α-GAL) and raffinose synthase (RAFS) were significantly elevated in raffinose-treated aged seeds. These findings suggest that exogenous raffinose induces the expression of α-GAL and RAFS, thereby providing energy and reducing excessive reactive oxygen species (ROS), which in turn promotes the germination of artificially aged seeds. This study provides a theoretical foundation for enhancing seed vigor and extending seed longevity in crop management. |
| format | Article |
| id | doaj-art-d28e3c18e35f4d4082e8a9bc60b72fe0 |
| institution | Kabale University |
| issn | 2073-4395 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj-art-d28e3c18e35f4d4082e8a9bc60b72fe02024-12-27T14:04:09ZengMDPI AGAgronomy2073-43952024-11-011412284310.3390/agronomy14122843Raffinose Priming Improves Seed Vigor by ROS Scavenging, RAFS, and α-GAL Activity in Aged Waxy CornMin Zhu0Ru Xiao1Tong Yu2Tao Guo3Xuemei Zhong4Jianzhou Qu5Wanli Du6Wei Xue7College of Agronomy, Specialty Corn Institute, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Agronomy, Specialty Corn Institute, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Agronomy, Specialty Corn Institute, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Agronomy, Specialty Corn Institute, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Agronomy, Specialty Corn Institute, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Agronomy, Specialty Corn Institute, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Agronomy, Specialty Corn Institute, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Agronomy, Specialty Corn Institute, Shenyang Agricultural University, Shenyang 110866, ChinaRaffinose family oligosaccharides (RFOs) are known to benefit plants under stress conditions; however, the role of exogenous raffinose in seed germination remains poorly understood. In this study, we investigated the potential role of raffinose in promoting seed germination and elucidated the underlying mechanisms. The results showed that artificial aging significantly reduced the germination rate and vigor of waxy corn seeds. Conversely, exogenous raffinose significantly enhanced the germination of these artificially aged seeds. Exogenous raffinose significantly reduced the levels of reactive oxygen species (O<sub>2</sub><sup>−</sup> and H<sub>2</sub>O<sub>2</sub>) and enhanced the activity of antioxidant enzymes, including superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT). Additionally, the levels of α-galactosidase (α-GAL) and raffinose synthase (RAFS) were significantly elevated in raffinose-treated aged seeds. These findings suggest that exogenous raffinose induces the expression of α-GAL and RAFS, thereby providing energy and reducing excessive reactive oxygen species (ROS), which in turn promotes the germination of artificially aged seeds. This study provides a theoretical foundation for enhancing seed vigor and extending seed longevity in crop management.https://www.mdpi.com/2073-4395/14/12/2843<i>Zea mays</i>artificial agingraffinoseseed vigorseed primingwaxy corn |
| spellingShingle | Min Zhu Ru Xiao Tong Yu Tao Guo Xuemei Zhong Jianzhou Qu Wanli Du Wei Xue Raffinose Priming Improves Seed Vigor by ROS Scavenging, RAFS, and α-GAL Activity in Aged Waxy Corn Agronomy <i>Zea mays</i> artificial aging raffinose seed vigor seed priming waxy corn |
| title | Raffinose Priming Improves Seed Vigor by ROS Scavenging, RAFS, and α-GAL Activity in Aged Waxy Corn |
| title_full | Raffinose Priming Improves Seed Vigor by ROS Scavenging, RAFS, and α-GAL Activity in Aged Waxy Corn |
| title_fullStr | Raffinose Priming Improves Seed Vigor by ROS Scavenging, RAFS, and α-GAL Activity in Aged Waxy Corn |
| title_full_unstemmed | Raffinose Priming Improves Seed Vigor by ROS Scavenging, RAFS, and α-GAL Activity in Aged Waxy Corn |
| title_short | Raffinose Priming Improves Seed Vigor by ROS Scavenging, RAFS, and α-GAL Activity in Aged Waxy Corn |
| title_sort | raffinose priming improves seed vigor by ros scavenging rafs and α gal activity in aged waxy corn |
| topic | <i>Zea mays</i> artificial aging raffinose seed vigor seed priming waxy corn |
| url | https://www.mdpi.com/2073-4395/14/12/2843 |
| work_keys_str_mv | AT minzhu raffinoseprimingimprovesseedvigorbyrosscavengingrafsandagalactivityinagedwaxycorn AT ruxiao raffinoseprimingimprovesseedvigorbyrosscavengingrafsandagalactivityinagedwaxycorn AT tongyu raffinoseprimingimprovesseedvigorbyrosscavengingrafsandagalactivityinagedwaxycorn AT taoguo raffinoseprimingimprovesseedvigorbyrosscavengingrafsandagalactivityinagedwaxycorn AT xuemeizhong raffinoseprimingimprovesseedvigorbyrosscavengingrafsandagalactivityinagedwaxycorn AT jianzhouqu raffinoseprimingimprovesseedvigorbyrosscavengingrafsandagalactivityinagedwaxycorn AT wanlidu raffinoseprimingimprovesseedvigorbyrosscavengingrafsandagalactivityinagedwaxycorn AT weixue raffinoseprimingimprovesseedvigorbyrosscavengingrafsandagalactivityinagedwaxycorn |