Characterization of Strip1 Expression in Mouse Cochlear Hair Cells
Striatin-interacting protein 1 (Strip1) is a core component of the striatin interacting phosphatase and kinase (STRIPAK) complex, which is involved in embryogenesis and development, circadian rhythms, type 2 diabetes, and cancer progression. However, the expression and role of Strip1 in the mammalia...
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Frontiers Media S.A.
2021-04-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2021.625867/full |
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| author | Shasha Zhang Ying Dong Ruiying Qiang Yuan Zhang Xiaoli Zhang Yin Chen Pei Jiang Xiangyu Ma Leilei Wu Jingru Ai Xia Gao Pengjun Wang Jie Chen Renjie Chai Renjie Chai Renjie Chai Renjie Chai |
| author_facet | Shasha Zhang Ying Dong Ruiying Qiang Yuan Zhang Xiaoli Zhang Yin Chen Pei Jiang Xiangyu Ma Leilei Wu Jingru Ai Xia Gao Pengjun Wang Jie Chen Renjie Chai Renjie Chai Renjie Chai Renjie Chai |
| author_sort | Shasha Zhang |
| collection | DOAJ |
| description | Striatin-interacting protein 1 (Strip1) is a core component of the striatin interacting phosphatase and kinase (STRIPAK) complex, which is involved in embryogenesis and development, circadian rhythms, type 2 diabetes, and cancer progression. However, the expression and role of Strip1 in the mammalian cochlea remains unclear. Here we studied the expression and function of Strip1 in the mouse cochlea by using Strip1 knockout mice. We first found that the mRNA and protein expression of Strip1 increases as mice age starting from postnatal day (P) 3 and reaches its highest expression level at P30 and that the expression of Strip1 can be detected by immunofluorescent staining starting from P14 only in cochlear HCs, and not in supporting cells (SCs). Next, we crossed Strip1 heterozygous knockout (Strip +/−) mice to obtain Strip1 homozygous knockout (Strip1−/−) mice for studying the role of Strip1 in cochlear HCs. However, no Strip1−/− mice were obtained and the ratio of Strip +/− to Strip1+/+ mice per litter was about 2:1, which suggested that homozygous Strip1 knockout is embryonic lethal. We measured hearing function and counted the HC number in P30 and P60 Strip +/− mice and found that they had normal hearing ability and HC numbers compared to Strip1+/+ mice. Our study suggested that Strip1 probably play important roles in HC development and maturation, which needs further study in the future. |
| format | Article |
| id | doaj-art-d27a333f0d554e5ebe0fa0811ececa66 |
| institution | Kabale University |
| issn | 1664-8021 |
| language | English |
| publishDate | 2021-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj-art-d27a333f0d554e5ebe0fa0811ececa662024-12-20T14:43:52ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-04-011210.3389/fgene.2021.625867625867Characterization of Strip1 Expression in Mouse Cochlear Hair CellsShasha Zhang0Ying Dong1Ruiying Qiang2Yuan Zhang3Xiaoli Zhang4Yin Chen5Pei Jiang6Xiangyu Ma7Leilei Wu8Jingru Ai9Xia Gao10Pengjun Wang11Jie Chen12Renjie Chai13Renjie Chai14Renjie Chai15Renjie Chai16State Key Laboratory of Bioelectronics, School of Life Sciences and Technology, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, ChinaState Key Laboratory of Bioelectronics, School of Life Sciences and Technology, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, ChinaState Key Laboratory of Bioelectronics, School of Life Sciences and Technology, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, ChinaState Key Laboratory of Bioelectronics, School of Life Sciences and Technology, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, ChinaJiangsu Provincial Key Medical Discipline (Laboratory), Department of Otolaryngology Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, ChinaJiangsu Provincial Key Medical Discipline (Laboratory), Department of Otolaryngology Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, ChinaState Key Laboratory of Bioelectronics, School of Life Sciences and Technology, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, ChinaState Key Laboratory of Bioelectronics, School of Life Sciences and Technology, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, ChinaState Key Laboratory of Bioelectronics, School of Life Sciences and Technology, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, ChinaState Key Laboratory of Bioelectronics, School of Life Sciences and Technology, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, ChinaJiangsu Provincial Key Medical Discipline (Laboratory), Department of Otolaryngology Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, ChinaDepartment of Otorhinolaryngology, Affiliated Sixth People’s Hospital of Shanghai Jiao Tong University, Shanghai, ChinaJiangsu Provincial Key Medical Discipline (Laboratory), Department of Otolaryngology Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, ChinaState Key Laboratory of Bioelectronics, School of Life Sciences and Technology, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, ChinaCo-Innovation Center of Neuroregeneration, Nantong University, Nantong, ChinaInstitute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, ChinaBeijing Key Laboratory of Neural Regeneration and Repair, Capital Medical University, Beijing, ChinaStriatin-interacting protein 1 (Strip1) is a core component of the striatin interacting phosphatase and kinase (STRIPAK) complex, which is involved in embryogenesis and development, circadian rhythms, type 2 diabetes, and cancer progression. However, the expression and role of Strip1 in the mammalian cochlea remains unclear. Here we studied the expression and function of Strip1 in the mouse cochlea by using Strip1 knockout mice. We first found that the mRNA and protein expression of Strip1 increases as mice age starting from postnatal day (P) 3 and reaches its highest expression level at P30 and that the expression of Strip1 can be detected by immunofluorescent staining starting from P14 only in cochlear HCs, and not in supporting cells (SCs). Next, we crossed Strip1 heterozygous knockout (Strip +/−) mice to obtain Strip1 homozygous knockout (Strip1−/−) mice for studying the role of Strip1 in cochlear HCs. However, no Strip1−/− mice were obtained and the ratio of Strip +/− to Strip1+/+ mice per litter was about 2:1, which suggested that homozygous Strip1 knockout is embryonic lethal. We measured hearing function and counted the HC number in P30 and P60 Strip +/− mice and found that they had normal hearing ability and HC numbers compared to Strip1+/+ mice. Our study suggested that Strip1 probably play important roles in HC development and maturation, which needs further study in the future.https://www.frontiersin.org/articles/10.3389/fgene.2021.625867/fullhair cellcochleainner earexpressionStrip1 |
| spellingShingle | Shasha Zhang Ying Dong Ruiying Qiang Yuan Zhang Xiaoli Zhang Yin Chen Pei Jiang Xiangyu Ma Leilei Wu Jingru Ai Xia Gao Pengjun Wang Jie Chen Renjie Chai Renjie Chai Renjie Chai Renjie Chai Characterization of Strip1 Expression in Mouse Cochlear Hair Cells Frontiers in Genetics hair cell cochlea inner ear expression Strip1 |
| title | Characterization of Strip1 Expression in Mouse Cochlear Hair Cells |
| title_full | Characterization of Strip1 Expression in Mouse Cochlear Hair Cells |
| title_fullStr | Characterization of Strip1 Expression in Mouse Cochlear Hair Cells |
| title_full_unstemmed | Characterization of Strip1 Expression in Mouse Cochlear Hair Cells |
| title_short | Characterization of Strip1 Expression in Mouse Cochlear Hair Cells |
| title_sort | characterization of strip1 expression in mouse cochlear hair cells |
| topic | hair cell cochlea inner ear expression Strip1 |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2021.625867/full |
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