Skin-iDISCO+: An optimized tissue-clearing and labeling protocol for morphometric analysis of human cutaneous vasculature
Summary: Tissue clearing enables deep imaging of long biological structures using light microscopy approaches. Protocols such as iDISCO are not currently available for human skin. Here, we present Skin-iDISCO+, a tissue-clearing and labeling protocol for morphometric analysis of human cutaneous vasc...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724006890 |
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| author | Léa Pechtimaldjian Muriel Cario Jérémie Teillon Sébastien Marais Sébastien Dupichaud Hamid-Reza Rezvani François Moisan |
| author_facet | Léa Pechtimaldjian Muriel Cario Jérémie Teillon Sébastien Marais Sébastien Dupichaud Hamid-Reza Rezvani François Moisan |
| author_sort | Léa Pechtimaldjian |
| collection | DOAJ |
| description | Summary: Tissue clearing enables deep imaging of long biological structures using light microscopy approaches. Protocols such as iDISCO are not currently available for human skin. Here, we present Skin-iDISCO+, a tissue-clearing and labeling protocol for morphometric analysis of human cutaneous vasculature. We describe steps for processing and pre-treating tissue, immunostaining, clearing, and imaging using confocal and light-sheet fluorescence microscopy. We then detail procedures for the reconstruction of networks and the extraction of morphometric parameters of the skin vasculature.For complete details on the use and execution of this protocol, please refer to Renier et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-d25424559fc74f04848ffa8f085e795e |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-d25424559fc74f04848ffa8f085e795e2024-12-22T05:29:47ZengElsevierSTAR Protocols2666-16672025-03-0161103524Skin-iDISCO+: An optimized tissue-clearing and labeling protocol for morphometric analysis of human cutaneous vasculatureLéa Pechtimaldjian0Muriel Cario1Jérémie Teillon2Sébastien Marais3Sébastien Dupichaud4Hamid-Reza Rezvani5François Moisan6University Bordeaux, Inserm, Bordeaux Institute of Oncology (BRIC), UMR 1312, F-33076 Bordeaux, France; Corresponding authorUniversity Bordeaux, Inserm, Bordeaux Institute of Oncology (BRIC), UMR 1312, F-33076 Bordeaux, FranceUniversity Bordeaux Imaging Center (BIC), Photonic Microscopy Unit, Bordeaux, FranceUniversity Bordeaux Imaging Center (BIC), Photonic Microscopy Unit, Bordeaux, FranceCarl Zeiss SAS, ZEISS Group, Rueil-Malmaison, FranceUniversity Bordeaux, Inserm, Bordeaux Institute of Oncology (BRIC), UMR 1312, F-33076 Bordeaux, FranceUniversity Bordeaux, Inserm, Bordeaux Institute of Oncology (BRIC), UMR 1312, F-33076 Bordeaux, France; Corresponding authorSummary: Tissue clearing enables deep imaging of long biological structures using light microscopy approaches. Protocols such as iDISCO are not currently available for human skin. Here, we present Skin-iDISCO+, a tissue-clearing and labeling protocol for morphometric analysis of human cutaneous vasculature. We describe steps for processing and pre-treating tissue, immunostaining, clearing, and imaging using confocal and light-sheet fluorescence microscopy. We then detail procedures for the reconstruction of networks and the extraction of morphometric parameters of the skin vasculature.For complete details on the use and execution of this protocol, please refer to Renier et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724006890classification description: health sciencesmicroscopyantibody |
| spellingShingle | Léa Pechtimaldjian Muriel Cario Jérémie Teillon Sébastien Marais Sébastien Dupichaud Hamid-Reza Rezvani François Moisan Skin-iDISCO+: An optimized tissue-clearing and labeling protocol for morphometric analysis of human cutaneous vasculature STAR Protocols classification description: health sciences microscopy antibody |
| title | Skin-iDISCO+: An optimized tissue-clearing and labeling protocol for morphometric analysis of human cutaneous vasculature |
| title_full | Skin-iDISCO+: An optimized tissue-clearing and labeling protocol for morphometric analysis of human cutaneous vasculature |
| title_fullStr | Skin-iDISCO+: An optimized tissue-clearing and labeling protocol for morphometric analysis of human cutaneous vasculature |
| title_full_unstemmed | Skin-iDISCO+: An optimized tissue-clearing and labeling protocol for morphometric analysis of human cutaneous vasculature |
| title_short | Skin-iDISCO+: An optimized tissue-clearing and labeling protocol for morphometric analysis of human cutaneous vasculature |
| title_sort | skin idisco an optimized tissue clearing and labeling protocol for morphometric analysis of human cutaneous vasculature |
| topic | classification description: health sciences microscopy antibody |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724006890 |
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