Effects of Aqueous Leaf Extract of Mimosa pudica on Insect Repellent–Induced Injury in the Trachea of Adult Wistar Rats
The use of insect repellent in occupational settings can lead to insecticide poisoning, a serious health hazard that can have severe consequences, including morbidity and mortality, if left untreated. The aim of this study was to investigate the effects of aqueous leaf extract of Mimosa pudica on in...
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| Format: | Article |
| Language: | English |
| Published: |
Hammer Head Production Limited
2025-05-01
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| Series: | Sokoto Journal of Medical Laboratory Science |
| Subjects: | |
| Online Access: | https://sokjmls.com.ng/index.php/SJMLS/article/view/643 |
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| Summary: | The use of insect repellent in occupational settings can lead to insecticide poisoning, a serious health hazard that can have severe consequences, including morbidity and mortality, if left untreated. The aim of this study was to investigate the effects of aqueous leaf extract of Mimosa pudica on insect repellent-induced injury in the adult Wistar rats. Thirty (30) adult Wistar rats (male and female) weighing between 236 g and 266 g were divided into five (5) groups of six (6) rats each. Group A rats were administered feed and water only. Group B rats were exposed to 2 puffs of insect repellent (10 ml/puff) at a concentration of 100 mg/m3, administered at 8 am by inhalation via fume distributor glass-chamber of dimensions 32.5 cm3 × 32.5 cm3 × 16.5 cm3 for 1 hour daily. Group C rats received 500mg/kg body weight per day (BWT/D) of Mimosa pudica. Groups D and E rats were exposed to 2 puffs of insect repellent (10 ml/puff) at a concentration of 100 mg/m3, administered at 8 am by inhalation via fume distributor glass-chamber of dimensions 32.5 cm3 × 32.5 cm3 × 16.5 cm3 for 1 hour daily and received 250mg/kg BWT/D (low dose) and 500mg/kg BWT/D (high dose) of Mimosa pudica The dosages of the extract were given for 30 consecutive days via an orogastric tube. The weights of the animals in each group were taken and recorded weekly and the difference between them and previous weights were noted. At the end of 30th day of exposure, the Wistar rats were weighed and euthanized under chloroform anesthesia. Blood samples were taken through cardiac puncture into plain specimen bottles for biochemical analysis. The trachea was harvested and processed for histological examination. The data obtained were analyzed using the one-way Analysis of Variance, with level of significance set at P<0.05. Histopathological examination confirmed normal tracheal architecture in the control group. The tracheal tissue of rats in Group C, which received 500mg of extract only, appeared normal, with intact mucosa, sub-epithelium, and cartilage. There were observable histological variations in the tracheal histoarchitecture of the exposed rats in Group B, D and E which include patchy mucosal ulceration, sub epithelial infiltrates of inflammatory cells, focal sub-epithelial oedema, focal mucosal erosion, focal sub-epithelial infiltrates of inflammatory cells, focal congestion and vasodilatation (evidence of tracheitis). This study revealed that exposure to insect repellent has detrimental effects on rat health, including significant weight reduction (p<0.05), biochemical alterations indicative of renal impairment and acidosis. Additionally, our results showed that Mimosa pudica did not exert a protective effect against tracheitis induced by insect repellent in Wistar rats. Given similar biological responses, these effects of insect repellent on rats can be reasonably extrapolated to humans, highlighting the importance of p r o t e c t i v e m e a s u r e s f o r i n d i v i d u a l s occupationally exposed to insect repellent. |
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| ISSN: | 2536-7153 |