Study of the Effect of Nutrient Environment on the Biological Characteristics of Fusarium solani Clinical Corneal Isolates
Purpose. The biological characteristics of Fusarium solani clinical corneal isolates growing in different nutritional conditions in vitro were studied in order to find out the key point of pathogenicity. Methods. Five kinds of media with different glucose and nitrogen concentrations were prepared as...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2021/6666303 |
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| _version_ | 1841524665870188544 |
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| author | Dalan Jing Yingyu Li Ziyuan Liu Chen Huang Pei Zhang Wei Wang |
| author_facet | Dalan Jing Yingyu Li Ziyuan Liu Chen Huang Pei Zhang Wei Wang |
| author_sort | Dalan Jing |
| collection | DOAJ |
| description | Purpose. The biological characteristics of Fusarium solani clinical corneal isolates growing in different nutritional conditions in vitro were studied in order to find out the key point of pathogenicity. Methods. Five kinds of media with different glucose and nitrogen concentrations were prepared as the liquid and solid forms. The clinical isolates were as follows: 2 Fusarium solani strains. The clinical corneal isolates and the standard strains were inoculated in the solid and liquid media. They were all incubated at 296 for 96 h and observed at defined time points. The optical density was recorded to generate the growth curves in liquid media. Morphologic changes of colonies in the solid media were determined under the light microscope. Results. The clinical isolates of Fusarium solani showed stronger reproductive capacity in the abominable nutritional condition. Besides, when the glucose concentration in the medium was consistent with the glucose concentration of aqueous in diabetic patients, the clinical isolates would show the biological features of quicker growth rate and stronger reproductive capacity. Conclusions. Nitrogen source is essential for fungus reproduction. The clinical isolates showed stronger environmental adaptability under different nutritional conditions and more sensitive to environmental changes. |
| format | Article |
| id | doaj-art-d18637bfc1414dd6848f03481a0b814e |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-d18637bfc1414dd6848f03481a0b814e2025-02-03T05:47:40ZengWileyJournal of Ophthalmology2090-004X2090-00582021-01-01202110.1155/2021/66663036666303Study of the Effect of Nutrient Environment on the Biological Characteristics of Fusarium solani Clinical Corneal IsolatesDalan Jing0Yingyu Li1Ziyuan Liu2Chen Huang3Pei Zhang4Wei Wang5Department of Ophthalmology, Peking University Third Hospital, Beijing, ChinaDepartment of Ophthalmology, Peking University Third Hospital, Beijing, ChinaDepartment of Ophthalmology, Peking University Third Hospital, Beijing, ChinaDepartment of Ophthalmology, Peking University Third Hospital, Beijing, ChinaDepartment of Ophthalmology, Peking University Third Hospital, Beijing, ChinaDepartment of Ophthalmology, Peking University Third Hospital, Beijing, ChinaPurpose. The biological characteristics of Fusarium solani clinical corneal isolates growing in different nutritional conditions in vitro were studied in order to find out the key point of pathogenicity. Methods. Five kinds of media with different glucose and nitrogen concentrations were prepared as the liquid and solid forms. The clinical isolates were as follows: 2 Fusarium solani strains. The clinical corneal isolates and the standard strains were inoculated in the solid and liquid media. They were all incubated at 296 for 96 h and observed at defined time points. The optical density was recorded to generate the growth curves in liquid media. Morphologic changes of colonies in the solid media were determined under the light microscope. Results. The clinical isolates of Fusarium solani showed stronger reproductive capacity in the abominable nutritional condition. Besides, when the glucose concentration in the medium was consistent with the glucose concentration of aqueous in diabetic patients, the clinical isolates would show the biological features of quicker growth rate and stronger reproductive capacity. Conclusions. Nitrogen source is essential for fungus reproduction. The clinical isolates showed stronger environmental adaptability under different nutritional conditions and more sensitive to environmental changes.http://dx.doi.org/10.1155/2021/6666303 |
| spellingShingle | Dalan Jing Yingyu Li Ziyuan Liu Chen Huang Pei Zhang Wei Wang Study of the Effect of Nutrient Environment on the Biological Characteristics of Fusarium solani Clinical Corneal Isolates Journal of Ophthalmology |
| title | Study of the Effect of Nutrient Environment on the Biological Characteristics of Fusarium solani Clinical Corneal Isolates |
| title_full | Study of the Effect of Nutrient Environment on the Biological Characteristics of Fusarium solani Clinical Corneal Isolates |
| title_fullStr | Study of the Effect of Nutrient Environment on the Biological Characteristics of Fusarium solani Clinical Corneal Isolates |
| title_full_unstemmed | Study of the Effect of Nutrient Environment on the Biological Characteristics of Fusarium solani Clinical Corneal Isolates |
| title_short | Study of the Effect of Nutrient Environment on the Biological Characteristics of Fusarium solani Clinical Corneal Isolates |
| title_sort | study of the effect of nutrient environment on the biological characteristics of fusarium solani clinical corneal isolates |
| url | http://dx.doi.org/10.1155/2021/6666303 |
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