The Comprehensive Root Metabolite–Rhizomicrobiota Response Patterns of <i>Rhododendron delavayi</i> (<i>R. delavayi</i>) to Waterlogging Stress and Post–Waterlogging Recovery
Waterlogging is a critical abiotic stressor that significantly impacts plant growth. Plants under waterlogging stress release metabolic signals that recruit rhizosphere microorganisms and enhance stress resistance. However, the mechanisms through which the non-adaptive species <i>R. delavayi&l...
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2025-07-01
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| author | Jing Tang Qingqing Huang Qian Wang Fei Shan Shaolong Wu Ximin Zhang Ming Tang Yin Yi |
| author_facet | Jing Tang Qingqing Huang Qian Wang Fei Shan Shaolong Wu Ximin Zhang Ming Tang Yin Yi |
| author_sort | Jing Tang |
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| description | Waterlogging is a critical abiotic stressor that significantly impacts plant growth. Plants under waterlogging stress release metabolic signals that recruit rhizosphere microorganisms and enhance stress resistance. However, the mechanisms through which the non-adaptive species <i>R. delavayi</i> responds to waterlogging stress via the synergistic interaction between root metabolites and rhizosphere microbiota remain poorly elucidated. Here, we employed pot experiments to characterize the responses of the root metabolite–microbiota complex in <i>R. delavayi</i> during waterlogging stress and subsequent recovery. Our results revealed that waterlogging altered the root morphology, the root metabolite profile, rhizosphere microbial diversity and network complexity, and these effects persisted during recovery. A significant correlation between root metabolites and the rhizosphere microbial community structure during waterlogging stress and recovery. Importantly, some differentially accumulated metabolites had significant effects on the assembly of rhizosphere microbes. Most of the core microbes in the rhizosphere microbial community under waterlogging and post–waterlogging recovery treatment were likely beneficial bacteria. Based on these findings, we propose a model for how root metabolites and rhizosphere microbes interact to help <i>R. delavayi</i> cope with waterlogging and recover. Based on these findings, we propose a possible response pattern of root metabolites and rhizosphere microbiota complex in <i>R. delavayi</i> under waterlogging stress and recovery. This work provides new insights into the synergistic mechanisms enhancing plant waterlogging tolerance and highlights the potential of harnessing rhizosphere microbiota to improve resilience in rhododendrons. |
| format | Article |
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| institution | Kabale University |
| issn | 2311-7524 |
| language | English |
| publishDate | 2025-07-01 |
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| series | Horticulturae |
| spelling | doaj-art-d178b8fc22f3429fb6c4d4317064c6242025-08-20T03:58:26ZengMDPI AGHorticulturae2311-75242025-07-0111777010.3390/horticulturae11070770The Comprehensive Root Metabolite–Rhizomicrobiota Response Patterns of <i>Rhododendron delavayi</i> (<i>R. delavayi</i>) to Waterlogging Stress and Post–Waterlogging RecoveryJing Tang0Qingqing Huang1Qian Wang2Fei Shan3Shaolong Wu4Ximin Zhang5Ming Tang6Yin Yi7Key Laboratory of Plant Physiology and Development Regulation, School of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaKey Laboratory of Plant Physiology and Development Regulation, School of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaKey Laboratory of Plant Physiology and Development Regulation, School of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaKey Laboratory of Environment Friendly Management on Alpine Rhododendron Diseases and Pests of Institutions of Higher Learning in Guizhou Province, Guizhou Normal University, Guiyang 550025, ChinaKey Laboratory of Plant Physiology and Development Regulation, School of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaKey Laboratory of Plant Physiology and Development Regulation, School of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaKey Laboratory of Plant Physiology and Development Regulation, School of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaKey Laboratory of Plant Physiology and Development Regulation, School of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaWaterlogging is a critical abiotic stressor that significantly impacts plant growth. Plants under waterlogging stress release metabolic signals that recruit rhizosphere microorganisms and enhance stress resistance. However, the mechanisms through which the non-adaptive species <i>R. delavayi</i> responds to waterlogging stress via the synergistic interaction between root metabolites and rhizosphere microbiota remain poorly elucidated. Here, we employed pot experiments to characterize the responses of the root metabolite–microbiota complex in <i>R. delavayi</i> during waterlogging stress and subsequent recovery. Our results revealed that waterlogging altered the root morphology, the root metabolite profile, rhizosphere microbial diversity and network complexity, and these effects persisted during recovery. A significant correlation between root metabolites and the rhizosphere microbial community structure during waterlogging stress and recovery. Importantly, some differentially accumulated metabolites had significant effects on the assembly of rhizosphere microbes. Most of the core microbes in the rhizosphere microbial community under waterlogging and post–waterlogging recovery treatment were likely beneficial bacteria. Based on these findings, we propose a model for how root metabolites and rhizosphere microbes interact to help <i>R. delavayi</i> cope with waterlogging and recover. Based on these findings, we propose a possible response pattern of root metabolites and rhizosphere microbiota complex in <i>R. delavayi</i> under waterlogging stress and recovery. This work provides new insights into the synergistic mechanisms enhancing plant waterlogging tolerance and highlights the potential of harnessing rhizosphere microbiota to improve resilience in rhododendrons.https://www.mdpi.com/2311-7524/11/7/770rhododendronwaterlogging stressroot–rhizomicrobiota complexbeneficial bacteriamulti–omics |
| spellingShingle | Jing Tang Qingqing Huang Qian Wang Fei Shan Shaolong Wu Ximin Zhang Ming Tang Yin Yi The Comprehensive Root Metabolite–Rhizomicrobiota Response Patterns of <i>Rhododendron delavayi</i> (<i>R. delavayi</i>) to Waterlogging Stress and Post–Waterlogging Recovery Horticulturae rhododendron waterlogging stress root–rhizomicrobiota complex beneficial bacteria multi–omics |
| title | The Comprehensive Root Metabolite–Rhizomicrobiota Response Patterns of <i>Rhododendron delavayi</i> (<i>R. delavayi</i>) to Waterlogging Stress and Post–Waterlogging Recovery |
| title_full | The Comprehensive Root Metabolite–Rhizomicrobiota Response Patterns of <i>Rhododendron delavayi</i> (<i>R. delavayi</i>) to Waterlogging Stress and Post–Waterlogging Recovery |
| title_fullStr | The Comprehensive Root Metabolite–Rhizomicrobiota Response Patterns of <i>Rhododendron delavayi</i> (<i>R. delavayi</i>) to Waterlogging Stress and Post–Waterlogging Recovery |
| title_full_unstemmed | The Comprehensive Root Metabolite–Rhizomicrobiota Response Patterns of <i>Rhododendron delavayi</i> (<i>R. delavayi</i>) to Waterlogging Stress and Post–Waterlogging Recovery |
| title_short | The Comprehensive Root Metabolite–Rhizomicrobiota Response Patterns of <i>Rhododendron delavayi</i> (<i>R. delavayi</i>) to Waterlogging Stress and Post–Waterlogging Recovery |
| title_sort | comprehensive root metabolite rhizomicrobiota response patterns of i rhododendron delavayi i i r delavayi i to waterlogging stress and post waterlogging recovery |
| topic | rhododendron waterlogging stress root–rhizomicrobiota complex beneficial bacteria multi–omics |
| url | https://www.mdpi.com/2311-7524/11/7/770 |
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