Multivariate optimization of Selaginellae Herba carbonization processing and its comparative analysis of antioxidant activity between crude and processed products
Objective: To optimize Selaginellae Herba Carbonisata (SH-Ca) processing using Box-Behnken Design Response Surface Methodology (BBD-RSM) combined with Analytic Hierarchy Process (AHP) and Entropy Weight Method (EWM), and comparatively evaluate its antioxidant enhancement over crude herb (SH-Cr) Meth...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-08-01
|
| Series: | Journal of Agriculture and Food Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666154325004016 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849254261247967232 |
|---|---|
| author | Wenting Zhong Xi Wang Hui Wang Feng Gao Menghua Wu Zhiguo Ma Tulin Lu Hui Cao Ying Zhang |
| author_facet | Wenting Zhong Xi Wang Hui Wang Feng Gao Menghua Wu Zhiguo Ma Tulin Lu Hui Cao Ying Zhang |
| author_sort | Wenting Zhong |
| collection | DOAJ |
| description | Objective: To optimize Selaginellae Herba Carbonisata (SH-Ca) processing using Box-Behnken Design Response Surface Methodology (BBD-RSM) combined with Analytic Hierarchy Process (AHP) and Entropy Weight Method (EWM), and comparatively evaluate its antioxidant enhancement over crude herb (SH-Cr) Methods: Processing parameters (input quantity, temperature, time) were optimized via BBD-RSM-AHP-EWM, with quality assessed by carbon adsorption capacity (CAC), total flavonoid content (TFC), chromatic aberration value (CAV), and appearance characteristics scoring (ACS). Additionally, the antioxidant capacities of SH-Cr and SH-Ca were evaluated using the DPPH radical scavenging activity, OH radical scavenging activity, ABTS-based total antioxidant capacity (T-AOC), and FRAP-based T-AOC assays. Results: AHP and EWM were applied to assign appropriate weights to these indicators, with ACS (58.27 %) being the most critical parameter, followed by TFC (24.33 %), CAV (13.54 %), and ACS (3.86 %). Model validation confirmed that the optimized conditions (an input quantity of 28 g, a temperature of 315 ± 15 °C, a processing time of 9.5 min) produced consistent results. While SH-Cr showed superior DPPH radical scavenging at high concentrations, SH-Ca exhibited more stable but generally lower scavenging activity across all tested concentrations. In contrast, SH-Ca displayed significantly enhanced OH radical scavenging activity (P < 0.05) and FRAP-based T-AOC compared to SH-Cr (P < 0.05), with its most notable advantage in ABTS-based T-AOC (P < 0.05) at high concentrations. Conclusion: The integrated BBD-RSM-AHP-EWM approach enabled optimal processing of SH-Ca, with comparative analysis revealing that SH-Ca exhibited superior efficacy against radical-induced oxidative damage whereas SH-Cr showed stronger direct radical quenching capacity. These findings provide insights for developing natural antioxidants with customized properties. |
| format | Article |
| id | doaj-art-d12e0a3a054b45568869f3b9824779e5 |
| institution | Kabale University |
| issn | 2666-1543 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Agriculture and Food Research |
| spelling | doaj-art-d12e0a3a054b45568869f3b9824779e52025-08-20T03:56:04ZengElsevierJournal of Agriculture and Food Research2666-15432025-08-012210203010.1016/j.jafr.2025.102030Multivariate optimization of Selaginellae Herba carbonization processing and its comparative analysis of antioxidant activity between crude and processed productsWenting Zhong0Xi Wang1Hui Wang2Feng Gao3Menghua Wu4Zhiguo Ma5Tulin Lu6Hui Cao7Ying Zhang8College of Pharmacy, Jinan University, Guangzhou, 511443, Guangdong, China; Research Center for Traditional Chinese Medicine of Lingnan (Southern China), Jinan University, Guangzhou, 511443, Guangdong, ChinaCollege of Pharmacy, Jinan University, Guangzhou, 511443, Guangdong, China; Research Center for Traditional Chinese Medicine of Lingnan (Southern China), Jinan University, Guangzhou, 511443, Guangdong, ChinaCollege of Pharmacy, Jinan University, Guangzhou, 511443, Guangdong, China; Research Center for Traditional Chinese Medicine of Lingnan (Southern China), Jinan University, Guangzhou, 511443, Guangdong, ChinaCollege of Pharmacy, Jinan University, Guangzhou, 511443, Guangdong, China; Research Center for Traditional Chinese Medicine of Lingnan (Southern China), Jinan University, Guangzhou, 511443, Guangdong, ChinaResearch Center for Traditional Chinese Medicine of Lingnan (Southern China), Jinan University, Guangzhou, 511443, Guangdong, China; National Engineering Research Center for Modernization of Traditional Chinese Medicine Lingnan Resources Branch, Guangzhou, 511443, Guangdong, China; Guangdong Key Lab of Traditional Chinese Medicine Information Technology, Guangzhou, 511443, Guangdong, ChinaResearch Center for Traditional Chinese Medicine of Lingnan (Southern China), Jinan University, Guangzhou, 511443, Guangdong, China; National Engineering Research Center for Modernization of Traditional Chinese Medicine Lingnan Resources Branch, Guangzhou, 511443, Guangdong, China; Guangdong Key Lab of Traditional Chinese Medicine Information Technology, Guangzhou, 511443, Guangdong, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, ChinaResearch Center for Traditional Chinese Medicine of Lingnan (Southern China), Jinan University, Guangzhou, 511443, Guangdong, China; National Engineering Research Center for Modernization of Traditional Chinese Medicine Lingnan Resources Branch, Guangzhou, 511443, Guangdong, China; Guangdong Key Lab of Traditional Chinese Medicine Information Technology, Guangzhou, 511443, Guangdong, China; Corresponding author. College of Pharmacy, Jinan University, Guangzhou, 511443, Guangdong, China.Research Center for Traditional Chinese Medicine of Lingnan (Southern China), Jinan University, Guangzhou, 511443, Guangdong, China; National Engineering Research Center for Modernization of Traditional Chinese Medicine Lingnan Resources Branch, Guangzhou, 511443, Guangdong, China; Guangdong Key Lab of Traditional Chinese Medicine Information Technology, Guangzhou, 511443, Guangdong, China; Corresponding author. College of Pharmacy, Jinan University, Guangzhou, 511443, Guangdong, China.Objective: To optimize Selaginellae Herba Carbonisata (SH-Ca) processing using Box-Behnken Design Response Surface Methodology (BBD-RSM) combined with Analytic Hierarchy Process (AHP) and Entropy Weight Method (EWM), and comparatively evaluate its antioxidant enhancement over crude herb (SH-Cr) Methods: Processing parameters (input quantity, temperature, time) were optimized via BBD-RSM-AHP-EWM, with quality assessed by carbon adsorption capacity (CAC), total flavonoid content (TFC), chromatic aberration value (CAV), and appearance characteristics scoring (ACS). Additionally, the antioxidant capacities of SH-Cr and SH-Ca were evaluated using the DPPH radical scavenging activity, OH radical scavenging activity, ABTS-based total antioxidant capacity (T-AOC), and FRAP-based T-AOC assays. Results: AHP and EWM were applied to assign appropriate weights to these indicators, with ACS (58.27 %) being the most critical parameter, followed by TFC (24.33 %), CAV (13.54 %), and ACS (3.86 %). Model validation confirmed that the optimized conditions (an input quantity of 28 g, a temperature of 315 ± 15 °C, a processing time of 9.5 min) produced consistent results. While SH-Cr showed superior DPPH radical scavenging at high concentrations, SH-Ca exhibited more stable but generally lower scavenging activity across all tested concentrations. In contrast, SH-Ca displayed significantly enhanced OH radical scavenging activity (P < 0.05) and FRAP-based T-AOC compared to SH-Cr (P < 0.05), with its most notable advantage in ABTS-based T-AOC (P < 0.05) at high concentrations. Conclusion: The integrated BBD-RSM-AHP-EWM approach enabled optimal processing of SH-Ca, with comparative analysis revealing that SH-Ca exhibited superior efficacy against radical-induced oxidative damage whereas SH-Cr showed stronger direct radical quenching capacity. These findings provide insights for developing natural antioxidants with customized properties.http://www.sciencedirect.com/science/article/pii/S2666154325004016Analytical Hierarchy processAntioxidant activityProcessing conditionsResponse surface methodologySelaginellae Herba |
| spellingShingle | Wenting Zhong Xi Wang Hui Wang Feng Gao Menghua Wu Zhiguo Ma Tulin Lu Hui Cao Ying Zhang Multivariate optimization of Selaginellae Herba carbonization processing and its comparative analysis of antioxidant activity between crude and processed products Journal of Agriculture and Food Research Analytical Hierarchy process Antioxidant activity Processing conditions Response surface methodology Selaginellae Herba |
| title | Multivariate optimization of Selaginellae Herba carbonization processing and its comparative analysis of antioxidant activity between crude and processed products |
| title_full | Multivariate optimization of Selaginellae Herba carbonization processing and its comparative analysis of antioxidant activity between crude and processed products |
| title_fullStr | Multivariate optimization of Selaginellae Herba carbonization processing and its comparative analysis of antioxidant activity between crude and processed products |
| title_full_unstemmed | Multivariate optimization of Selaginellae Herba carbonization processing and its comparative analysis of antioxidant activity between crude and processed products |
| title_short | Multivariate optimization of Selaginellae Herba carbonization processing and its comparative analysis of antioxidant activity between crude and processed products |
| title_sort | multivariate optimization of selaginellae herba carbonization processing and its comparative analysis of antioxidant activity between crude and processed products |
| topic | Analytical Hierarchy process Antioxidant activity Processing conditions Response surface methodology Selaginellae Herba |
| url | http://www.sciencedirect.com/science/article/pii/S2666154325004016 |
| work_keys_str_mv | AT wentingzhong multivariateoptimizationofselaginellaeherbacarbonizationprocessinganditscomparativeanalysisofantioxidantactivitybetweencrudeandprocessedproducts AT xiwang multivariateoptimizationofselaginellaeherbacarbonizationprocessinganditscomparativeanalysisofantioxidantactivitybetweencrudeandprocessedproducts AT huiwang multivariateoptimizationofselaginellaeherbacarbonizationprocessinganditscomparativeanalysisofantioxidantactivitybetweencrudeandprocessedproducts AT fenggao multivariateoptimizationofselaginellaeherbacarbonizationprocessinganditscomparativeanalysisofantioxidantactivitybetweencrudeandprocessedproducts AT menghuawu multivariateoptimizationofselaginellaeherbacarbonizationprocessinganditscomparativeanalysisofantioxidantactivitybetweencrudeandprocessedproducts AT zhiguoma multivariateoptimizationofselaginellaeherbacarbonizationprocessinganditscomparativeanalysisofantioxidantactivitybetweencrudeandprocessedproducts AT tulinlu multivariateoptimizationofselaginellaeherbacarbonizationprocessinganditscomparativeanalysisofantioxidantactivitybetweencrudeandprocessedproducts AT huicao multivariateoptimizationofselaginellaeherbacarbonizationprocessinganditscomparativeanalysisofantioxidantactivitybetweencrudeandprocessedproducts AT yingzhang multivariateoptimizationofselaginellaeherbacarbonizationprocessinganditscomparativeanalysisofantioxidantactivitybetweencrudeandprocessedproducts |