Genetic Analysis of <i>HSP70</i> and <i>HSF3</i> Polymorphisms and Their Associations with the Egg Production Traits of Bangladeshi Hilly Chickens
In warm environments, thermoregulation in poultry is controlled by heat shock protein 70 (HSP70), whose expression is controlled by heat shock factor 3 (HSF3). Although the association between genetic polymorphisms in these genes and thermotolerance as well as reproductive traits has been extensivel...
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MDPI AG
2024-12-01
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| author | Md Yousuf Ali Shakila Faruque Sadequllah Ahmadi Takeshi Ohkubo |
| author_facet | Md Yousuf Ali Shakila Faruque Sadequllah Ahmadi Takeshi Ohkubo |
| author_sort | Md Yousuf Ali |
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| description | In warm environments, thermoregulation in poultry is controlled by heat shock protein 70 (HSP70), whose expression is controlled by heat shock factor 3 (HSF3). Although the association between genetic polymorphisms in these genes and thermotolerance as well as reproductive traits has been extensively studied in mammals, the association has not yet been studied in poultry. This study aimed to explore the relationship between single-nucleotide polymorphisms (SNPs) in these genes and the egg production traits of Bangladeshi hilly chickens. Sequencing and allele-specific PCR (AS-PCR) were used to detect new SNPs and perform genotyping. We identified two novel SNPs (G-399A and A-68G) in the 5′-flanking regions of HSP70 that were significantly associated with egg numbers (ENs) at 161–190 days and increased egg weight (EW) at 40 weeks of age. Furthermore, three SNPs in HSP70 (A258G, C276G and C1431A) and one SNP in HSF3 (A-1388G) were associated with EN at different ages. The haplotype and combined genotypic effects of these two genes were found to be associated with age at sexual maturity (ASM), EN, EW, and body weight at ASM. The identified SNPs and their corresponding haplotypes may be useful in selective breeding to enhance the productivity of chickens in warm environments. |
| format | Article |
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| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-d12d996e1e8e4e7db3f73c7dfdc84d3c2024-12-27T14:05:28ZengMDPI AGAnimals2076-26152024-12-011424355210.3390/ani14243552Genetic Analysis of <i>HSP70</i> and <i>HSF3</i> Polymorphisms and Their Associations with the Egg Production Traits of Bangladeshi Hilly ChickensMd Yousuf Ali0Shakila Faruque1Sadequllah Ahmadi2Takeshi Ohkubo3Bangladesh Livestock Research Institute, Savar, Dhaka 1341, BangladeshBangladesh Livestock Research Institute, Savar, Dhaka 1341, BangladeshUnited Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu 183-8538, JapanUnited Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu 183-8538, JapanIn warm environments, thermoregulation in poultry is controlled by heat shock protein 70 (HSP70), whose expression is controlled by heat shock factor 3 (HSF3). Although the association between genetic polymorphisms in these genes and thermotolerance as well as reproductive traits has been extensively studied in mammals, the association has not yet been studied in poultry. This study aimed to explore the relationship between single-nucleotide polymorphisms (SNPs) in these genes and the egg production traits of Bangladeshi hilly chickens. Sequencing and allele-specific PCR (AS-PCR) were used to detect new SNPs and perform genotyping. We identified two novel SNPs (G-399A and A-68G) in the 5′-flanking regions of HSP70 that were significantly associated with egg numbers (ENs) at 161–190 days and increased egg weight (EW) at 40 weeks of age. Furthermore, three SNPs in HSP70 (A258G, C276G and C1431A) and one SNP in HSF3 (A-1388G) were associated with EN at different ages. The haplotype and combined genotypic effects of these two genes were found to be associated with age at sexual maturity (ASM), EN, EW, and body weight at ASM. The identified SNPs and their corresponding haplotypes may be useful in selective breeding to enhance the productivity of chickens in warm environments.https://www.mdpi.com/2076-2615/14/24/3552chickenegg productionHSF3HSP70SNPs |
| spellingShingle | Md Yousuf Ali Shakila Faruque Sadequllah Ahmadi Takeshi Ohkubo Genetic Analysis of <i>HSP70</i> and <i>HSF3</i> Polymorphisms and Their Associations with the Egg Production Traits of Bangladeshi Hilly Chickens Animals chicken egg production HSF3 HSP70 SNPs |
| title | Genetic Analysis of <i>HSP70</i> and <i>HSF3</i> Polymorphisms and Their Associations with the Egg Production Traits of Bangladeshi Hilly Chickens |
| title_full | Genetic Analysis of <i>HSP70</i> and <i>HSF3</i> Polymorphisms and Their Associations with the Egg Production Traits of Bangladeshi Hilly Chickens |
| title_fullStr | Genetic Analysis of <i>HSP70</i> and <i>HSF3</i> Polymorphisms and Their Associations with the Egg Production Traits of Bangladeshi Hilly Chickens |
| title_full_unstemmed | Genetic Analysis of <i>HSP70</i> and <i>HSF3</i> Polymorphisms and Their Associations with the Egg Production Traits of Bangladeshi Hilly Chickens |
| title_short | Genetic Analysis of <i>HSP70</i> and <i>HSF3</i> Polymorphisms and Their Associations with the Egg Production Traits of Bangladeshi Hilly Chickens |
| title_sort | genetic analysis of i hsp70 i and i hsf3 i polymorphisms and their associations with the egg production traits of bangladeshi hilly chickens |
| topic | chicken egg production HSF3 HSP70 SNPs |
| url | https://www.mdpi.com/2076-2615/14/24/3552 |
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