Metabolic Fingerprinting of Blood and Urine of Dairy Cows Affected by Bovine Leukemia Virus: A Mass Spectrometry Approach
Objectives: This study investigated metabolic changes associated with bovine leukemia virus (BLV) infection in dairy cows, focusing on pre-parturition alterations. Methods: Metabolite identification in serum and urine samples was performed using a targeted metabolomics method, employing the TMIC Pri...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/14/11/624 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846153035491835904 |
|---|---|
| author | Dawid Tobolski Grzegorz Zwierzchowski Roman Wójcik Klevis Haxhiaj David S. Wishart Burim N. Ametaj |
| author_facet | Dawid Tobolski Grzegorz Zwierzchowski Roman Wójcik Klevis Haxhiaj David S. Wishart Burim N. Ametaj |
| author_sort | Dawid Tobolski |
| collection | DOAJ |
| description | Objectives: This study investigated metabolic changes associated with bovine leukemia virus (BLV) infection in dairy cows, focusing on pre-parturition alterations. Methods: Metabolite identification in serum and urine samples was performed using a targeted metabolomics method, employing the TMIC Prime kit in combination with flow injection analysis and liquid chromatography–tandem mass spectrometry. Results: Of 145 cows examined, 42 (28.9%) were BLV-seropositive. Around 38% of infected cows showed high somatic cell counts indicative of subclinical mastitis, with 15 experiencing additional health issues such as ketosis, milk fever, and lameness. Despite these conditions, no significant differences in milk yield or composition were observed between the infected and control groups. Metabolomic analysis conducted at −8 and −4 weeks prepartum revealed significant metabolic differences between BLV-infected and healthy cows. At −8 weeks, 30 serum metabolites were altered, including sphingomyelins, lysophosphatidylcholines, amino acids, and acylcarnitines, suggesting disruptions in membrane integrity, energy metabolism, and immune function indicative of early neoplastic transformations. By −4 weeks, the number of altered metabolites decreased to 17, continuing to reflect metabolic disruptions in cows with leukemia. Multivariate analysis highlighted distinct metabolic profiles between infected and control cows, identifying key discriminating metabolites such as choline, aspartic acid, phenylalanine, and arginine. Urine metabolomics revealed significant prepartum shifts in metabolites related to glucose, asymmetric dimethylarginine, and pyruvic acid, among others. Conclusions: The research confirmed metabolomics’ efficacy in defining a BLV infection metabolic profile, elucidating leukosis-associated metabolic disruptions. This approach facilitates the identification of BLV-infected cows and enhances understanding of infection pathophysiology, providing a foundation for advanced management and intervention strategies in dairy herds. The study underscores the profound impact of leukosis on metabolic processes and highlights urine metabolomics’ utility in non-invasively detecting BLV infection, offering the potential for improved herd health management. |
| format | Article |
| id | doaj-art-d119b7b1a09d44a181d56f248461033a |
| institution | Kabale University |
| issn | 2218-1989 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj-art-d119b7b1a09d44a181d56f248461033a2024-11-26T18:13:12ZengMDPI AGMetabolites2218-19892024-11-01141162410.3390/metabo14110624Metabolic Fingerprinting of Blood and Urine of Dairy Cows Affected by Bovine Leukemia Virus: A Mass Spectrometry ApproachDawid Tobolski0Grzegorz Zwierzchowski1Roman Wójcik2Klevis Haxhiaj3David S. Wishart4Burim N. Ametaj5Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences, 02-787 Warsaw, PolandDepartment of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, CanadaFaculty of Veterinary Medicine, University of Warmia and Mazury, 1a Oczapowskiego Str., 10-719 Olsztyn, PolandDepartment of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, CanadaDepartments of Biological and Computer Sciences, University of Alberta, Edmonton, AB T6G 2P5, CanadaDepartment of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, CanadaObjectives: This study investigated metabolic changes associated with bovine leukemia virus (BLV) infection in dairy cows, focusing on pre-parturition alterations. Methods: Metabolite identification in serum and urine samples was performed using a targeted metabolomics method, employing the TMIC Prime kit in combination with flow injection analysis and liquid chromatography–tandem mass spectrometry. Results: Of 145 cows examined, 42 (28.9%) were BLV-seropositive. Around 38% of infected cows showed high somatic cell counts indicative of subclinical mastitis, with 15 experiencing additional health issues such as ketosis, milk fever, and lameness. Despite these conditions, no significant differences in milk yield or composition were observed between the infected and control groups. Metabolomic analysis conducted at −8 and −4 weeks prepartum revealed significant metabolic differences between BLV-infected and healthy cows. At −8 weeks, 30 serum metabolites were altered, including sphingomyelins, lysophosphatidylcholines, amino acids, and acylcarnitines, suggesting disruptions in membrane integrity, energy metabolism, and immune function indicative of early neoplastic transformations. By −4 weeks, the number of altered metabolites decreased to 17, continuing to reflect metabolic disruptions in cows with leukemia. Multivariate analysis highlighted distinct metabolic profiles between infected and control cows, identifying key discriminating metabolites such as choline, aspartic acid, phenylalanine, and arginine. Urine metabolomics revealed significant prepartum shifts in metabolites related to glucose, asymmetric dimethylarginine, and pyruvic acid, among others. Conclusions: The research confirmed metabolomics’ efficacy in defining a BLV infection metabolic profile, elucidating leukosis-associated metabolic disruptions. This approach facilitates the identification of BLV-infected cows and enhances understanding of infection pathophysiology, providing a foundation for advanced management and intervention strategies in dairy herds. The study underscores the profound impact of leukosis on metabolic processes and highlights urine metabolomics’ utility in non-invasively detecting BLV infection, offering the potential for improved herd health management.https://www.mdpi.com/2218-1989/14/11/624dairy cowbovine leukemia virusmetabolomicsserumurineDI/LC-MS/M |
| spellingShingle | Dawid Tobolski Grzegorz Zwierzchowski Roman Wójcik Klevis Haxhiaj David S. Wishart Burim N. Ametaj Metabolic Fingerprinting of Blood and Urine of Dairy Cows Affected by Bovine Leukemia Virus: A Mass Spectrometry Approach Metabolites dairy cow bovine leukemia virus metabolomics serum urine DI/LC-MS/M |
| title | Metabolic Fingerprinting of Blood and Urine of Dairy Cows Affected by Bovine Leukemia Virus: A Mass Spectrometry Approach |
| title_full | Metabolic Fingerprinting of Blood and Urine of Dairy Cows Affected by Bovine Leukemia Virus: A Mass Spectrometry Approach |
| title_fullStr | Metabolic Fingerprinting of Blood and Urine of Dairy Cows Affected by Bovine Leukemia Virus: A Mass Spectrometry Approach |
| title_full_unstemmed | Metabolic Fingerprinting of Blood and Urine of Dairy Cows Affected by Bovine Leukemia Virus: A Mass Spectrometry Approach |
| title_short | Metabolic Fingerprinting of Blood and Urine of Dairy Cows Affected by Bovine Leukemia Virus: A Mass Spectrometry Approach |
| title_sort | metabolic fingerprinting of blood and urine of dairy cows affected by bovine leukemia virus a mass spectrometry approach |
| topic | dairy cow bovine leukemia virus metabolomics serum urine DI/LC-MS/M |
| url | https://www.mdpi.com/2218-1989/14/11/624 |
| work_keys_str_mv | AT dawidtobolski metabolicfingerprintingofbloodandurineofdairycowsaffectedbybovineleukemiavirusamassspectrometryapproach AT grzegorzzwierzchowski metabolicfingerprintingofbloodandurineofdairycowsaffectedbybovineleukemiavirusamassspectrometryapproach AT romanwojcik metabolicfingerprintingofbloodandurineofdairycowsaffectedbybovineleukemiavirusamassspectrometryapproach AT klevishaxhiaj metabolicfingerprintingofbloodandurineofdairycowsaffectedbybovineleukemiavirusamassspectrometryapproach AT davidswishart metabolicfingerprintingofbloodandurineofdairycowsaffectedbybovineleukemiavirusamassspectrometryapproach AT burimnametaj metabolicfingerprintingofbloodandurineofdairycowsaffectedbybovineleukemiavirusamassspectrometryapproach |