Optimizing seedling production for the conservation of the threatened Dyckia rariflora (Bromeliaceae)
Abstract Objectives In vitro seed propagation can enhance plant species growth and enable the rapid production of seedlings while preserving genetic variability. This study aimed to develop in vitro seed propagation and acclimatization protocols for Dyckia rariflora to support conservation efforts o...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-11-01
|
| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13104-024-07001-5 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846148135475216384 |
|---|---|
| author | Giselly Mota da Silva Evandro Alves Vieira Luiz Palhares Neto Leilane Barbara Gomes Markus Gastauer Silvio J. Ramos Cecílio Frois Caldeira |
| author_facet | Giselly Mota da Silva Evandro Alves Vieira Luiz Palhares Neto Leilane Barbara Gomes Markus Gastauer Silvio J. Ramos Cecílio Frois Caldeira |
| author_sort | Giselly Mota da Silva |
| collection | DOAJ |
| description | Abstract Objectives In vitro seed propagation can enhance plant species growth and enable the rapid production of seedlings while preserving genetic variability. This study aimed to develop in vitro seed propagation and acclimatization protocols for Dyckia rariflora to support conservation efforts of this bromeliad endemic to ferruginous campos rupestres. Seed germination and plant growth were tested using MS (Murashige & Skoog) culture medium with varying salt concentrations, sucrose levels, and the presence or absence of polyvinylpyrrolidone (PVP). Following these treatments, seedlings were acclimatized after removal from the controlled environment. Results Germination rates varied between 65 and 90%, unaffected by treatment. The highest germination speed index was in half MS salts without PVP, while full MS salts, sucrose, and PVP slowed germination. Half MS salts resulted in seedlings with greater height, more leaves, and longer roots. Complete MS salts were less effective. No seed oxidation was observed. After 120 days of acclimatization, survival rates exceeded 70%, with plants in half MS salts and 15 g sucrose showing the best growth. In vitro propagation of D. rariflora is viable for large-scale plant production, with half MS salt and sucrose concentrations, without PVP, recommended for better plant growth and cost reduction. |
| format | Article |
| id | doaj-art-d0e45d58b5814f849a8229513cd6b38e |
| institution | Kabale University |
| issn | 1756-0500 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj-art-d0e45d58b5814f849a8229513cd6b38e2024-12-01T12:08:16ZengBMCBMC Research Notes1756-05002024-11-011711610.1186/s13104-024-07001-5Optimizing seedling production for the conservation of the threatened Dyckia rariflora (Bromeliaceae)Giselly Mota da Silva0Evandro Alves Vieira1Luiz Palhares Neto2Leilane Barbara Gomes3Markus Gastauer4Silvio J. Ramos5Cecílio Frois Caldeira6Instituto Técnológico Vale, Boaventura da SilvaInstituto Técnológico Vale, Boaventura da SilvaUniversidade Estadual Do Sudoeste da BahiaCentro de Biodiversidade ValeInstituto Técnológico Vale, Boaventura da SilvaInstituto Técnológico Vale, Boaventura da SilvaInstituto Técnológico Vale, Boaventura da SilvaAbstract Objectives In vitro seed propagation can enhance plant species growth and enable the rapid production of seedlings while preserving genetic variability. This study aimed to develop in vitro seed propagation and acclimatization protocols for Dyckia rariflora to support conservation efforts of this bromeliad endemic to ferruginous campos rupestres. Seed germination and plant growth were tested using MS (Murashige & Skoog) culture medium with varying salt concentrations, sucrose levels, and the presence or absence of polyvinylpyrrolidone (PVP). Following these treatments, seedlings were acclimatized after removal from the controlled environment. Results Germination rates varied between 65 and 90%, unaffected by treatment. The highest germination speed index was in half MS salts without PVP, while full MS salts, sucrose, and PVP slowed germination. Half MS salts resulted in seedlings with greater height, more leaves, and longer roots. Complete MS salts were less effective. No seed oxidation was observed. After 120 days of acclimatization, survival rates exceeded 70%, with plants in half MS salts and 15 g sucrose showing the best growth. In vitro propagation of D. rariflora is viable for large-scale plant production, with half MS salt and sucrose concentrations, without PVP, recommended for better plant growth and cost reduction.https://doi.org/10.1186/s13104-024-07001-5Campos rupestresConservationGerminationAcclimatization |
| spellingShingle | Giselly Mota da Silva Evandro Alves Vieira Luiz Palhares Neto Leilane Barbara Gomes Markus Gastauer Silvio J. Ramos Cecílio Frois Caldeira Optimizing seedling production for the conservation of the threatened Dyckia rariflora (Bromeliaceae) BMC Research Notes Campos rupestres Conservation Germination Acclimatization |
| title | Optimizing seedling production for the conservation of the threatened Dyckia rariflora (Bromeliaceae) |
| title_full | Optimizing seedling production for the conservation of the threatened Dyckia rariflora (Bromeliaceae) |
| title_fullStr | Optimizing seedling production for the conservation of the threatened Dyckia rariflora (Bromeliaceae) |
| title_full_unstemmed | Optimizing seedling production for the conservation of the threatened Dyckia rariflora (Bromeliaceae) |
| title_short | Optimizing seedling production for the conservation of the threatened Dyckia rariflora (Bromeliaceae) |
| title_sort | optimizing seedling production for the conservation of the threatened dyckia rariflora bromeliaceae |
| topic | Campos rupestres Conservation Germination Acclimatization |
| url | https://doi.org/10.1186/s13104-024-07001-5 |
| work_keys_str_mv | AT gisellymotadasilva optimizingseedlingproductionfortheconservationofthethreateneddyckiarariflorabromeliaceae AT evandroalvesvieira optimizingseedlingproductionfortheconservationofthethreateneddyckiarariflorabromeliaceae AT luizpalharesneto optimizingseedlingproductionfortheconservationofthethreateneddyckiarariflorabromeliaceae AT leilanebarbaragomes optimizingseedlingproductionfortheconservationofthethreateneddyckiarariflorabromeliaceae AT markusgastauer optimizingseedlingproductionfortheconservationofthethreateneddyckiarariflorabromeliaceae AT silviojramos optimizingseedlingproductionfortheconservationofthethreateneddyckiarariflorabromeliaceae AT ceciliofroiscaldeira optimizingseedlingproductionfortheconservationofthethreateneddyckiarariflorabromeliaceae |